Abstract
The release of gentamicin adenine mononucleotide transferase during single, cold, osmotic-shock treatment of Escherichia coli K-12 W677/HJR66 is not always maximal. The quantity of gentamicin adenine mononucleotide transferase in osmotic extracts could be significantly increased when a double-shock procedure was performed.
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Selected References
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- Benveniste R., Davies J. R-factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics. FEBS Lett. 1971 May 20;14(5):293–296. doi: 10.1016/0014-5793(71)80282-x. [DOI] [PubMed] [Google Scholar]
- Forrey A. W., Blair A., O'Neill M., Cutler R. E., Christopher T. G. Enzymatic assay for gentamicin. N Engl J Med. 1973 Jan 11;288(2):108–108. doi: 10.1056/nejm197301112880223. [DOI] [PubMed] [Google Scholar]
- Goldman P. R., Northrop D. B. Preparation of stable gentamicin adenylyl transferase of high specific activity. Biochem Biophys Res Commun. 1975 Oct 27;66(4):1408–1413. doi: 10.1016/0006-291x(75)90516-1. [DOI] [PubMed] [Google Scholar]
- Holmes R. K., Sanford J. P. Enzymatic assay for gentamicin and related aminoglycoside antibiotics. J Infect Dis. 1974 May;129(5):519–527. doi: 10.1093/infdis/129.5.519. [DOI] [PubMed] [Google Scholar]
- LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
- Nossal N. G., Heppel L. A. The release of enzymes by osmotic shock from Escherichia coli in exponential phase. J Biol Chem. 1966 Jul 10;241(13):3055–3062. [PubMed] [Google Scholar]
- Smith A. L., Smith D. H. Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin. J Infect Dis. 1974 Apr;129(4):391–401. doi: 10.1093/infdis/129.4.391. [DOI] [PubMed] [Google Scholar]
- Smith D. H., Van Otto B., Smith A. L. A rapid chemical assay for gentamicin. N Engl J Med. 1972 Mar 16;286(11):583–586. doi: 10.1056/NEJM197203162861106. [DOI] [PubMed] [Google Scholar]
- Tilton R. C., Murphy J. R., Mallett E. Assay for gentamicin. N Engl J Med. 1972 Nov 23;287(21):1100–1100. doi: 10.1056/nejm197211232872122. [DOI] [PubMed] [Google Scholar]