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. 1978 Jan;13(1):104–114. doi: 10.1128/aac.13.1.104

Actinomycin Biosynthesis by Protoplasts Derived from Streptomyces parvulus

Michael J M Hitchcock 1, Edward Katz 1
PMCID: PMC352192  PMID: 75712

Abstract

Conditions are described for the formation of protoplasts from Streptomyces parvulus that are able to synthesize actinomycin D de novo. Antibiotic synthesis by protoplasts, in contrast to that by mycelium, was sensitive to inhibition by actinomycin D and to a decrease in sucrose concentration. On the other hand, synthesis by mycelium was much more sensitive to inhibition by amino acid analogs (d-valine, cis-3-methylproline, and α-methyl-dl-tryptophan). In addition, the uptake of amino acids (l-methionine, sarcosine, and l- and d-valine) by protoplasts was significantly lower than that by mycelium. The advantages and limitations of using protoplasts for studying in vivo actinomycin synthesis are discussed.

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Selected References

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