VOLUME 287 (2012) PAGES 8782–8791
PAGE 8783:
The method used to stain with the primary anti-AQP2 antibody should be corrected as follows. In immunocytochemistry staining studies, the tissue was not incubated with the primary anti-AQP2 antibody (tagged with ATTO-550) along with the anti-TRPV4 primary antibody as stated but was added after the anti-TRPV4 primary antibody overnight preincubation period. After this overnight preincubation, anti-AQP2 was added along with the secondary anti-rabbit antibody (Cy2-labeled) for 3 h (at room temperature) and then washed and prepared as described. This approach abolished any labeling of the anti-AQP2 antibody (generated in rabbit) by the secondary anti-rabbit antibody (Cy2-labeled), as verified in separate studies, so that only anti-TRPV4 labeling by the secondary antibody was apparent.