Abstract
A clinical isolate of Haemophilus parainfluenzae resistant to chloramphenicol and tetracycline transferred both cam and tet determinants to Escherichia coli K-12 during mixed cultivation on solid media irrespective of the selection employed. The doubly resistant transconjugant was found to contain levels of the enzyme chloramphenicol acetyltransferase (CAT) comparable to those found in R plasmid-bearing chloramphenicol-resistant enteric bacteria. Purification of CAT from the transconjugant was achieved by affinity chromatography, and the electrophoretically homogeneous protein was compared with previously characterized CAT variants specified by R plasmids. Although the CAT associated with cam from H. parainfluenzae was found to be distinct from the three types described previously, its N-terminal peptide amino acid sequence was identical with that determined for a type II CAT. Attempts to demonstrate covalently closed circular deoxyribonucleic acid in the H. parainfluenzae donor and the E. coli transconjugant were unsuccessful. The cam and tet determinants were nontransmissible from E. coli but could be cotransferred following the introduction of a suitable conjugative plasmid.
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- Cavanagh P., Morris C. A., Mitchell N. J. Letter: Chloramphenicol resistance in haemophilus species. Lancet. 1975 Mar 22;1(7908):696–696. doi: 10.1016/s0140-6736(75)91810-3. [DOI] [PubMed] [Google Scholar]
- De Graaff J., Elwell L. P., Falkow S. Molecular nature of two beta-lactamase-specifying plasmids isolated from Haemophilus influenzae type b. J Bacteriol. 1976 Apr;126(1):439–446. doi: 10.1128/jb.126.1.439-446.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Elwell L. P., De Graaff J., Seibert D., Falkow S. Plasmid-linked ampicillin resistance in haempohilus influenza type b. Infect Immun. 1975 Aug;12(2):404–410. doi: 10.1128/iai.12.2.404-410.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Goldstein F. W., Boisivon A., Leclerc P., Acar J. F. Sensibilité d'Hemophilus sp. aux antibiotiques. transfert de résistance à Escherichia coli. Pathol Biol (Paris) 1977 May;25(5):323–332. [PubMed] [Google Scholar]
- Kattan S. Letter: Chloramphenicol-resistant Haemophilus. Lancet. 1976 Apr 10;1(7963):814–814. doi: 10.1016/s0140-6736(76)91663-9. [DOI] [PubMed] [Google Scholar]
- Shaw W. V. Chloramphenicol acetyltransferase from chloramphenicol-resistant bacteria. Methods Enzymol. 1975;43:737–755. doi: 10.1016/0076-6879(75)43141-x. [DOI] [PubMed] [Google Scholar]
- Van A. D., Bieth G., Bouanchaud D. H. Résistance plasmidique à la tétracycline chez Haemophilus influenzae. C R Acad Sci Hebd Seances Acad Sci D. 1975 Mar 10;280(10):1321–1323. [PubMed] [Google Scholar]
- Van A. D., Goldstein F., Acar J. F., Bouanchaud D. H. A transferable kanamycin resistance plasmid isolated from Haemophilus influenzae. Ann Microbiol (Paris) 1975 Apr;126(3):397–399. [PubMed] [Google Scholar]
- Winshell E., Shaw W. V. Kinetics of induction and purification of chloramphenicol acetyltransferase from chloramphenicol-resistant Staphylococcus aureus. J Bacteriol. 1969 Jun;98(3):1248–1257. doi: 10.1128/jb.98.3.1248-1257.1969. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Zaidenzaig Y., Shaw W. V. Affinity and hydrophobic chromatography of three variants of chloramphenicol acetyltransferases specified by R factors in Escherichia coli. FEBS Lett. 1976 Mar 1;62(3):266–271. doi: 10.1016/0014-5793(76)80072-5. [DOI] [PubMed] [Google Scholar]