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. 1978 Mar;13(3):394–398. doi: 10.1128/aac.13.3.394

Totally synthetic medium for susceptibility testing.

R M Lawrence, P D Hoeprich
PMCID: PMC352251  PMID: 122521

Abstract

The results of disk diffusion and plate dilution susceptibility testing of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis on media gelled with agar-agar or with a synthetic hydrogel were compared. Synthetic hydrogel can be combined with a totally defined synthetic amino acid medium to yield a reproducible, totally defined, synthetic solid medium without the antagonistic or booster effects of some components of agar. Such a medium could be used as a reference medium for susceptibility testing.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barry A. L., Effinger L. J. Performances of Mueller-Hinton agars prepared by three manufacturers. Am J Clin Pathol. 1974 Jul;62(1):113–117. doi: 10.1093/ajcp/62.1.113. [DOI] [PubMed] [Google Scholar]
  2. Bovallius A., Zacharias B. Variations in the metal content of some commercial media and their effect on microbial growth. Appl Microbiol. 1971 Sep;22(3):260–262. doi: 10.1128/am.22.3.260-262.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brenner V. C., Sherris J. C. Influence of different media and bloods on results of diffusion antibiotic susceptibility tests. Antimicrob Agents Chemother. 1972 Feb;1(2):116–122. doi: 10.1128/aac.1.2.116. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Ericsson H. M., Sherris J. C. Antibiotic sensitivity testing. Report of an international collaborative study. Acta Pathol Microbiol Scand B Microbiol Immunol. 1971;217(Suppl):1+–1+. [PubMed] [Google Scholar]
  5. Garrod L. P., Waterworth P. M. Effect of medium composition on the apparent sensitivity of Pseudomonas aeruginosa to gentamicin. J Clin Pathol. 1969 Sep;22(5):534–538. doi: 10.1136/jcp.22.5.534. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hanus F. J., Sands J. G., Bennett E. O. Antibiotic activity in the presence of agar. Appl Microbiol. 1967 Jan;15(1):31–34. doi: 10.1128/am.15.1.31-34.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kunin C. M., Edmondson W. P. Inhibitor of antibiotics in bacteriologic agar. Proc Soc Exp Biol Med. 1968 Oct;129(1):118–122. doi: 10.3181/00379727-129-33264. [DOI] [PubMed] [Google Scholar]
  8. Ley H. L., Jr, Mueller J. H. On the Isolation from Agar of an Inhibitor for Neisseria gonorrhoeae. J Bacteriol. 1946 Oct;52(4):453–460. doi: 10.1128/jb.52.4.453-460.1946. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reller L. B., Schoenknecht F. D., Kenny M. A., Sherris J. C. Antibiotic susceptibility testing of Pseudomonas aeruginosa: selection of a control strain and criteria for magnesium and calcium content in media. J Infect Dis. 1974 Nov;130(5):454–463. doi: 10.1093/infdis/130.5.454. [DOI] [PubMed] [Google Scholar]
  10. WEINBERG E. D. The mutual effects of antimicrobial compounds and metallic cations. Bacteriol Rev. 1957 Mar;21(1):46–68. doi: 10.1128/br.21.1.46-68.1957. [DOI] [PMC free article] [PubMed] [Google Scholar]

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