Abstract
A method for the rapid, quantitative determination of 5-fluorocytosine (5-FC) in serum by high-performance liquid chromatography (HPLC) has been developed. After initially ultrafiltrating the serum, a portion was injected onto a cation exchange column. 5-FC was separated by using an ammonium-phosphate buffer as the mobile phase and detected by ultraviolet absorption at 254 nm. Quantitation of 5-FC was based on the linear relationship between peak area in the chromatograms and known concentrations of 5-FC in a set of serum standards (prepared by adding from 10 to 200 micrograms of 5-FC to 1-ml aliquots of pooled human serum). This method was compared with the standard microbiological method for 5-FC. Advantages of the HPLC method include: Determination of 5-FC levels within 30 min; lack of interference from other antimicrobial drugs, particularly amphotericin B; more accurate determination of true 5-FC level, particularly at concentrations of less than 25 micrograms/ml or greater than 100 micrograms/ml; and ease with which the assay may be automated for routine use.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bennet J. E. Flucytosine. Ann Intern Med. 1977 Mar;86(3):319–321. doi: 10.7326/0003-4819-86-3-319. [DOI] [PubMed] [Google Scholar]
- Bennett J. E. Chemotherapy of systemic mycoses (first of two parts). N Engl J Med. 1974 Jan 3;290(1):30–32. doi: 10.1056/NEJM197401032900107. [DOI] [PubMed] [Google Scholar]
- Blair A. D., Forrey A. W., Meijsen B. T., Cutler R. E. Assay of flucytosine and furosemide by high-pressure liquid chromatography. J Pharm Sci. 1975 Aug;64(8):1334–1339. doi: 10.1002/jps.2600640816. [DOI] [PubMed] [Google Scholar]
- Block E. R., Bennett J. E. Pharmacological studies with 5-fluorocytosine. Antimicrob Agents Chemother. 1972 Jun;1(6):476–482. doi: 10.1128/aac.1.6.476. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Harding S. A., Johnson G. F., Solomon H. M. Gas-chromatographic determination of 5-fluorocytosine in human serum. Clin Chem. 1976 Jun;22(6):772–776. [PubMed] [Google Scholar]
- Kaspar R. L., Drutz D. J. Rapid, simple bioassay for 5-fluorocytosine in the presence of amphotericin B. Antimicrob Agents Chemother. 1975 Apr;7(4):462–465. doi: 10.1128/aac.7.4.462. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Koechlin B. A., Rubio F., Palmer S., Gabriel T., Duschinsky R. The metabolism of 5-fluorocytosine-2-14-C and of cytosine-14-C in the rat and the disposition of 5-fluorocytosine-2-14-C in man. Biochem Pharmacol. 1966 Apr;15(4):435–446. doi: 10.1016/0006-2952(66)90254-1. [DOI] [PubMed] [Google Scholar]
- Nilsson-Ehle I., Yoshikawa T. T., Edwards J. E., Schotz M. C., Guze L. B. Quantitation of amphotericin B with use of high-pressure liquid chromatography. J Infect Dis. 1977 Mar;135(3):414–422. doi: 10.1093/infdis/135.3.414. [DOI] [PubMed] [Google Scholar]