Abstract
A fluorescent erythromycin derivative was used to determine the binding of erythromycin derivatives to ribosomes. The assay is rapid, sensitive, and convenient. Because measurements are made in solution, they represent equilibrium conditions, unlike filter binding assays which perturb the equilibrium. Results correlate well with measurements made by other techniques.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Harris R., Pestka S. Studies on the formation of transfer ribonucleic acid-ribosome complexes. XXIV. Effects of antibiotics on binding of aminoacyl-oligonucleotides to ribosomes. J Biol Chem. 1973 Feb 25;248(4):1168–1174. [PubMed] [Google Scholar]
- Langlois R., Cantor C. R., Vince R., Pestka S. Interaction between the erythromycin and chloramphenicol binding sites on the Escherichica coli ribosome. Biochemistry. 1977 May 31;16(11):2349–2356. doi: 10.1021/bi00630a007. [DOI] [PubMed] [Google Scholar]
- Langlois R., Lee C. C., Cantor C. R., Vince R., Pestka S. The distance between two functionally significant regions of the 50 S Escherichia coli ribosome: the erythromycin binding site and proteins L7/L12. J Mol Biol. 1976 Sep 15;106(2):297–313. doi: 10.1016/0022-2836(76)90087-5. [DOI] [PubMed] [Google Scholar]
- LeMahieu R. A., Carson M., Kierstead R. W., Pestka S. Aromatic esters of 5-O-desosaminylerythronolide A oxime. J Med Chem. 1975 Aug;18(8):849–851. doi: 10.1021/jm00242a020. [DOI] [PubMed] [Google Scholar]
- Omura S., Nakagawa A., Sakakibara H., Okekawa O., Brandsch R. Structure-activity relationships among the O-acyl derivatives of leucomycin. Correlation of minimal inhibitory concentrations with binding to Escherichia coli ribosomes. J Med Chem. 1977 May;20(5):732–736. doi: 10.1021/jm00215a025. [DOI] [PubMed] [Google Scholar]
- Pestka S., LeMahieu R. A. Inhibition of [14C]chloramphenicol binding to Escherichia coli ribosomes by erythromycin derivatives. Antimicrob Agents Chemother. 1974 Jul;6(1):39–45. doi: 10.1128/aac.6.1.39. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pestka S., Lemahieu R. A. Effect of erythromycin analogues on binding of [14C]erythromycin to Escherichia coli ribosomes. Antimicrob Agents Chemother. 1974 Oct;6(4):479–488. doi: 10.1128/aac.6.4.479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pestka S., Nakagawa A., Omura S. Effect of leucomycins and analogues on binding [14C ]erythromycin to Escherichia coli ribosomes. Antimicrob Agents Chemother. 1974 Nov;6(5):606–612. doi: 10.1128/aac.6.5.606. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Pestka S. Studies on the formation of transfer ribonucleic acid-ribosome complexes. 3. The formation of peptide bonds by ribosomes in the absence of supernatant enzymes. J Biol Chem. 1968 May 25;243(10):2810–2820. [PubMed] [Google Scholar]
- Vince R., Weiss D., Pestka S. Binding of N-substituted erythromycyclamines to ribosomes. Antimicrob Agents Chemother. 1976 Jan;9(1):131–136. doi: 10.1128/aac.9.1.131. [DOI] [PMC free article] [PubMed] [Google Scholar]