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. 1978 Sep;14(3):439–443. doi: 10.1128/aac.14.3.439

High-Performance Liquid Chromatographic Assay of Chloramphenicol in Serum

Jeffrey R Koup †, Barbara Brodsky 1, Alan Lau 2, Thomas R Beam Jr 3
PMCID: PMC352477  PMID: 708020

Abstract

A new method for the analysis of serum chloramphenicol by reversed-phase, high-performance liquid chromatography (HPLC) is described. The method involves a preliminary extraction of 0.1 ml of serum with ethyl acetate containing an internal standard, chromatography with a reversed-phase C18 microparticulate column with an acetonitrile-acetate buffer mobile phase, and detection by measuring UV absorbance at 270 nm. Assay performance was compared with an existing microbiological assay. The HPLC method demonstrated both increased precision and increased sensitivity. The specificity of the HPLC method was also evaluated. The new method presents an alternative approach to the analysis of clinical specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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