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. 1978 Sep;14(3):488–492. doi: 10.1128/aac.14.3.488

Purification and the Ultrastructure of a Bacteriocin Produced from Shigella sonnei Strain 100052

Kazunobu Amako 1, Motoyuki Matsuguchi 2, Kenji Takeya 2, Emiko Tatsuta 1, Yasuyuki Takagi 3
PMCID: PMC352487  PMID: 360986

Abstract

A bacteriocin produced by strain 100052 of Shigella sonnei (shigellacin 52) was purified about 80-fold from a mitomycin C-induced culture supernatant. The purified preparation gave a single band on sodium dodecyl sulfate-gel electrophoresis with an approximate molecular weight of 10,000. The negatively stained electron micrograph of the purified bacteriocin preparation revealed the existence of three different particles. They were a small cylindrical tube (4.2 by 6.0 nm), a ring-shaped particle and a filamentous structure. The molecular weight calculated from the shape of the small tube was approximately 50,000. The latter two particles appeared to be constructed from subunits which were morphologically similar to the cylindrical tube. These results suggested that the morphological subunit of shigellacin 52 consisted of five chemical subunits with molecular weights of 10,000 each, and it assembled into two types of polymers, a ring-shaped particle and a filamentous structure, which seemed to be the main components of the purified shigellacin 52 preparation.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ABBOTT J. D., GRAHAM J. M. Colicine typing of Shigella sonnei. Mon Bull Minist Health Public Health Lab Serv. 1961 Mar;20:51–58. [PubMed] [Google Scholar]
  2. Amako K., Matsuguchi M., Takeya K. Particles found in the mitomycin-induced culture fluid of a strain of Shigella sonnei and their relation to the bacteriocin activity. Jpn J Microbiol. 1975 Dec;19(6):456–458. doi: 10.1111/j.1348-0421.1975.tb00964.x. [DOI] [PubMed] [Google Scholar]
  3. Amako K., Tokiwa H., Takeya K. A bacteriocin induced from a strain of Shigella sonnei. Jpn J Microbiol. 1970 Nov;14(6):505–507. [PubMed] [Google Scholar]
  4. Bradley D. E. Ultrastructure of bacteriophage and bacteriocins. Bacteriol Rev. 1967 Dec;31(4):230–314. doi: 10.1128/br.31.4.230-314.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. ENDO H., AYABE K., AMAKO K., TAKEYA K. INDUCIBLE PHAGE OF ESCHERICHIA COLI 15. Virology. 1965 Mar;25:469–471. doi: 10.1016/0042-6822(65)90067-x. [DOI] [PubMed] [Google Scholar]
  6. Ishii S. I., Nishi Y., Egami F. The fine structure of a pyocin. J Mol Biol. 1965 Sep;13(2):428–431. doi: 10.1016/s0022-2836(65)80107-3. [DOI] [PubMed] [Google Scholar]
  7. ORNSTEIN L. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY. Ann N Y Acad Sci. 1964 Dec 28;121:321–349. doi: 10.1111/j.1749-6632.1964.tb14207.x. [DOI] [PubMed] [Google Scholar]
  8. Takeya K., Minamishima Y., Ohnishi Y., Amako K. Rod-shaped pyocin 28. J Gen Virol. 1969 Mar;4(2):145–149. doi: 10.1099/0022-1317-4-2-145. [DOI] [PubMed] [Google Scholar]
  9. Weber K., Osborn M. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis. J Biol Chem. 1969 Aug 25;244(16):4406–4412. [PubMed] [Google Scholar]
  10. Wrigley N. G. The lattice spacing of crystalline catalase as an internal standard of length in electron microscopy. J Ultrastruct Res. 1968 Sep;24(5):454–464. doi: 10.1016/s0022-5320(68)80048-6. [DOI] [PubMed] [Google Scholar]

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