Table 1. Kinetic parameters for the reduction of quinone propionic acid derivatives by hNQO1.

Values reported are the mean ± one standard deviation for three independent determinations. [NADH] = 1.00 × 10⁻⁴ M in all cases. The van der Waals volumes were calculated according to the literature.⁸⁹

Quinone	Vmax (µmol·min−1·mg−1)	Km (µM)	kcat (s−1)	kcat/Km (M−1·s−1)	E1/2 vs. SHE (V)	van der Waals volume (Å3)
	88±7	41±8	45±4	1.1±0.2 × 106	0.095±0.001	251
	83±8	50±11	43±4	8.5±2.0 × 105	0.117±0.002	232
	42±5	447±102	22±3	4.8±1.2 × 104	0.098±0.001	258
	38±5	158±41	20.±3	1.2±0.4 × 105	0.047±0.002	249
	60±7	132±32	31±4	2.3±0.6 × 105	0.041±0.001	295
	14±1	376±87	7.2±0.5	1.9±0.5 × 104	0.128±0.001	282
	78±3	20±3	40.±2	2.0±0.3 × 106	0.144±0.001	232
	66±4	5±1	34±2	6.8±1.4 × 106	0.143±0.002	215