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. 1978 Oct;14(4):605–608. doi: 10.1128/aac.14.4.605

Iontophoretic Application of Adenine Arabinoside Monophosphate to Herpes Simplex Virus Type 1-Infected Hairless Mouse Skin

No-Hee Park †, Louis P Gangarosa 1,2, Byoung-Se Kwon 3, James M Hili 3
PMCID: PMC352514  PMID: 82423

Abstract

Several antiviral agents were applied topically or by iontophoresis to hairless mouse skin inoculated with herpes simplex virus type 1 (HSV-1), and the chemotherapeutic effectiveness was evaluated. Topical application of iododeoxyuridine, arabinoside A, and adenine arabinoside monophosphate (ara-AMP) moderately decreased the average lesion score, number of mice with paralysis, and number of mice dying in HSV-1-infected animals. Also, the mean survival time was moderately prolonged by topical application of those antiviral agents. When ara-AMP was applied by cathodal (−) iontophoresis to the HSV-1-infected skin, the average lesion score, number of mice with paralysis, and number of mice dying were greatly decreased. Furthermore, the mean survival time of mice was highly increased by cathodal (−) iontophoresis of ara-AMP. The therapeutic efficacy of ara-AMP iontophoresis was much superior to the topical application of iododeoxyuridine, arabinoside A, and ara-AMP. These data suggest that ara-AMP iontophoresis would be the method of choice for the management of HSV-1 skin lesions in hairless mice.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cohen S. S., Plunkett W. The utilization of nucleotides by animal cells. Ann N Y Acad Sci. 1975 Aug 8;255:269–286. doi: 10.1111/j.1749-6632.1975.tb29235.x. [DOI] [PubMed] [Google Scholar]
  2. Comeau M., Brummett R., Vernon J. Local anesthesia of the ear by iontophoresis. Arch Otolaryngol. 1973 Aug;98(2):114–120. doi: 10.1001/archotol.1973.00780020120012. [DOI] [PubMed] [Google Scholar]
  3. Constantine V. S., Francis R. D., Mason B. H. Experimental zoster-like herpes simplex in hairless mice. J Invest Dermatol. 1971 Mar;56(3):193–199. doi: 10.1111/1523-1747.ep12260802. [DOI] [PubMed] [Google Scholar]
  4. Drach J. C., Sandberg J. N., Shipman C., Jr Antiproliferative effects of 9-beta-d-arabinofuranosyladenine in a mammalian cell line devoid of adenosine deaminase activity. J Dent Res. 1977 Mar;56(3):275–288. doi: 10.1177/00220345770560031301. [DOI] [PubMed] [Google Scholar]
  5. Echols D. F., Norris C. H., Tabb H. G. Anesthesia of the ear by iontophoresis of lidocaine. Arch Otolaryngol. 1975 Jul;101(7):418–421. doi: 10.1001/archotol.1975.00780360018004. [DOI] [PubMed] [Google Scholar]
  6. Epley J. M. Modified technique of iontophoretic anesthesia for myringotomy in children. Arch Otolaryngol. 1977 Jun;103(6):358–360. doi: 10.1001/archotol.1977.00780230080013. [DOI] [PubMed] [Google Scholar]
  7. GIBSON L. E., COOKE R. E. A test for concentration of electrolytes in sweat in cystic fibrosis of the pancreas utilizing pilocarpine by iontophoresis. Pediatrics. 1959 Mar;23(3):545–549. [PubMed] [Google Scholar]
  8. Gangarosa L. P., Park N. H., Hill J. M. Iontophoretic assistance of 5-lodo-2'-deoxyuridine penetration into neonatal mouse skin and effects on DNA synthesis. Proc Soc Exp Biol Med. 1977 Mar;154(3):439–443. doi: 10.3181/00379727-154-39689. [DOI] [PubMed] [Google Scholar]
  9. Gangarosa L. P., Park N. H. Practical considerations in iontophoresis of fluoride for desensitizing dentin. J Prosthet Dent. 1978 Feb;39(2):173–178. doi: 10.1016/s0022-3913(78)80017-1. [DOI] [PubMed] [Google Scholar]
  10. Gangarosa L. P., Sr Iontophoresis for surface local anesthesia. J Am Dent Assoc. 1974 Jan;88(1):125–128. doi: 10.14219/jada.archive.1974.0038. [DOI] [PubMed] [Google Scholar]
  11. Hill J. M., Gangarosa L. P., Park N. H. Iontophoretic application of antiviral chemotherapeutic agents. Ann N Y Acad Sci. 1977 Mar 4;284:604–612. doi: 10.1111/j.1749-6632.1977.tb21994.x. [DOI] [PubMed] [Google Scholar]
  12. Kaufman H. E., Varnell E. D. Effect of 9-beta-D-arabinofuranosyladenine 5'-monophosphate and 9-beta-D-arabinofuranosylhypoxanthine 5'-monophosphate on experimental herpes simplex keratitis. Antimicrob Agents Chemother. 1976 Dec;10(6):885–888. doi: 10.1128/aac.10.6.885. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kurtz S. M., Fitzgerald J. E., Schardein J. L. Comparative animal toxicology of vidarabine and its 5'-monophosphate. Ann N Y Acad Sci. 1977 Mar 4;284:6–8. doi: 10.1111/j.1749-6632.1977.tb21931.x. [DOI] [PubMed] [Google Scholar]
  14. Lieberman M., Schafer T. W., Came P. E. Chemotherapy of cutaneous herpesvirus infection of hairless mice. J Invest Dermatol. 1973 Apr;60(4):203–206. doi: 10.1111/1523-1747.ep12724476. [DOI] [PubMed] [Google Scholar]
  15. Murthy K. S., Talim S. T., Singh I. A comparative evaluation of topical application and iontophoresis of sodium fluoride for desensitization of hypersensitive dentin. Oral Surg Oral Med Oral Pathol. 1973 Sep;36(3):448–458. doi: 10.1016/0030-4220(73)90227-2. [DOI] [PubMed] [Google Scholar]
  16. Nahmias A. J., Roizman B. Infection with herpes-simplex viruses 1 and 2. II. N Engl J Med. 1973 Oct 4;289(14):719–725. doi: 10.1056/NEJM197310042891404. [DOI] [PubMed] [Google Scholar]
  17. Park N. H., Gangarosa L. P., Hill J. M. Iontophoretic application of ara-AMP (9-beta-D-arabinofuranosyladenine-5'-monophosphate) into adult mouse skin. Proc Soc Exp Biol Med. 1977 Nov;156(2):326–329. doi: 10.3181/00379727-156-39929. [DOI] [PubMed] [Google Scholar]
  18. Plunkett W., Lapi L., Ortiz P. J., Cohen S. S. Penetration of mouse fibroblasts by the 5'-phosphate of 9-beta-D-arabinofuranosyladenine and incorporation of the nucleotide into DNA. Proc Natl Acad Sci U S A. 1974 Jan;71(1):73–77. doi: 10.1073/pnas.71.1.73. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Prusoff W. H., Ward D. C. Nucleoside analogs with antiviral activity. Biochem Pharmacol. 1976 Jun 1;25(11):1233–1239. doi: 10.1016/0006-2952(76)90083-6. [DOI] [PubMed] [Google Scholar]
  20. Schaeffer M. L., Bixler D., Yu P. L. The effectiveness of iontophoresis in reducing cervical hypersensitivity. J Periodontol. 1971 Nov;42(11):695–700. doi: 10.1902/jop.1971.42.11.695. [DOI] [PubMed] [Google Scholar]
  21. Sloan B. J., Kielty J. K., Miller F. A. Effect of a novel adenosine deaminase inhibitor (co-vidarabine, co-V) upon the antiviral activity in vitro and in vivo of vidarabine (Vira-Atm) for DNA virus replication. Ann N Y Acad Sci. 1977 Mar 4;284:60–80. doi: 10.1111/j.1749-6632.1977.tb21937.x. [DOI] [PubMed] [Google Scholar]
  22. Spruance S. L., Overall J. C., Jr, Kern E. R., Krueger G. G., Pliam V., Miller W. The natural history of recurrent herpes simplex labialis: implications for antiviral therapy. N Engl J Med. 1977 Jul 14;297(2):69–75. doi: 10.1056/NEJM197707142970201. [DOI] [PubMed] [Google Scholar]
  23. Whitley R. J., Soong S. J., Dolin R., Galasso G. J., Ch'ien L. T., Alford C. A. Adenine arabinoside therapy of biopsy-proved herpes simplex encephalitis. National Institute of Allergy and Infectious Diseases collaborative antiviral study. N Engl J Med. 1977 Aug 11;297(6):289–294. doi: 10.1056/NEJM197708112970601. [DOI] [PubMed] [Google Scholar]

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