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. Author manuscript; available in PMC: 2014 Feb 15.
Published in final edited form as: Oncogene. 2012 Sep 10;32(33):3877–3885. doi: 10.1038/onc.2012.392

Figure 1.

Figure 1

Angiogenic factors are upregulated in Smad1/5 dKO granulosa cell tumors. Real-time quantitative PCR was performed to determine expression levels of Pdgfa (A), Pdgfb (B), Vegfa (C) and Vegfb (D) in wild type mouse granulosa cells (GC), Smad1/5 dKO tumors (TU) and metastasis (MET). siRNA-mediated knockdown (+KD) of both Smad1 and Smad5 in mouse granulosa cells resulted in more than 85% reduction in Smad1 (E) and Smad5 (F) transcript levels compared to the cells transfected cells with a scrambled control siRNA (-KD). A significant increase in Pdgfa (G) expression is observed following Smad1/5 knockdown, without any change in Pdgfb (H) levels. Different letters above the bars indicate statistically different means by ANOVA and post hoc analysis (n=4; P< 0.05).