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. 1979 Feb;15(2):263–268. doi: 10.1128/aac.15.2.263

Factors Contributing to Resistance to Beta-Lactam Antibiotics in Bacteroides fragilis

Barbro Olsson 1,2, Kathrine Dornbusch 1,2, Carl Erik Nord 1,2
PMCID: PMC352644  PMID: 426517

Abstract

Fifteen strains of Bacteroides fragilis, five highly resistant, five moderately resistant, and five susceptible to benzylpenicillin and cephaloridine, were tested for β-lactamase production. In the highly resistant and the moderately resistant groups of strains, a correlation between formation of β-lactamase and minimal inhibitory concentrations was demonstrated. In the presence of the β-lactamase inhibitors clavulanic acid and CP-45,899 at concentrations of 1 μg/ml, the susceptibility to cephaloridine increased fourfold or more in the β-lactamase-producing strains. Crypticity measurements (β-lactamase activity broken/intact cells) with cephaloridine as substrate could indicate a diffusion barrier in the cell wall.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boman H. G., Nordström K., Normark S. Penicillin resistance in Escherichia coli K12: synergism between penicillinases and a barrier in the outer part of the envelope. Ann N Y Acad Sci. 1974 May 10;235(0):569–586. doi: 10.1111/j.1749-6632.1974.tb43291.x. [DOI] [PubMed] [Google Scholar]
  2. Darland G., Birnbaum J. Cefoxitin resistance to beta-lactamase: a major factor for susceptibility of bacteroides fragilis to the antibiotic. Antimicrob Agents Chemother. 1977 Apr;11(4):725–734. doi: 10.1128/aac.11.4.725. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Dornbusch K., Nord C. E., Olsson B. Antibiotic susceptibility testing of anaerobic bacteria by the standardized disc diffusion method with special reference to bacteroides fragilis. Scand J Infect Dis. 1975;7(1):59–66. doi: 10.3109/inf.1975.7.issue-1.11. [DOI] [PubMed] [Google Scholar]
  4. Nord C. E., Möllby R., Smyth C., Wadström T. Formation of phospholipase C and theta-haemolysin in pre-reduced media in batch anc continuous culture of Clostridium perfringens type A. J Gen Microbiol. 1974 Sep;84(1):117–127. doi: 10.1099/00221287-84-1-117. [DOI] [PubMed] [Google Scholar]
  5. Nord C. E., Olsson B., Dornbusch K. beta-lactamases in bacteroides. Scand J Infect Dis Suppl. 1978;(13):27–32. [PubMed] [Google Scholar]
  6. O'Callaghan C. H., Morris A., Kirby S. M., Shingler A. H. Novel method for detection of beta-lactamases by using a chromogenic cephalosporin substrate. Antimicrob Agents Chemother. 1972 Apr;1(4):283–288. doi: 10.1128/aac.1.4.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Olsson B., Dornbusch K., Nord C. E. Susceptibility to beta-lactam antibiotics and production of beta-lactamase in Bacteroides fragilis. Med Microbiol Immunol. 1977 Oct 7;163(3):183–194. doi: 10.1007/BF02126677. [DOI] [PubMed] [Google Scholar]
  8. Olsson B., Nord C. E., Wadström T. Formation of beta-lactamase in Bacteroides fragilis: cell-bound and extracellular activity. Antimicrob Agents Chemother. 1976 May;9(5):727–735. doi: 10.1128/aac.9.5.727. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Reading C., Cole M. Clavulanic acid: a beta-lactamase-inhiting beta-lactam from Streptomyces clavuligerus. Antimicrob Agents Chemother. 1977 May;11(5):852–857. doi: 10.1128/aac.11.5.852. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Richmond M. H., Clark D. C., Wotton S. Indirect method for assessing the penetration of beta-lactamase-nonsusceptible penicillins and cephalosporins in Escherichia coli strains. Antimicrob Agents Chemother. 1976 Aug;10(2):215–218. doi: 10.1128/aac.10.2.215. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Richmond M. H., Wotton S. Comparative study of seven cephalosporins: susceptibility to beta-lactamases and ability to penetrate the surface layers of Escherichia coli. Antimicrob Agents Chemother. 1976 Aug;10(2):219–222. doi: 10.1128/aac.10.2.219. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Sykes R. B., Matthew M. The beta-lactamases of gram-negative bacteria and their role in resistance to beta-lactam antibiotics. J Antimicrob Chemother. 1976 Jun;2(2):115–157. doi: 10.1093/jac/2.2.115. [DOI] [PubMed] [Google Scholar]
  13. Weinrich A. E., Del bene V. E. Beta-lactamase activity in anaerobic bacteria. Antimicrob Agents Chemother. 1976 Jul;10(1):106–111. doi: 10.1128/aac.10.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Wise R. Clavulanic acid and susceptibility of Bacteroides fragilis to penicillin. Lancet. 1977 Jul 16;2(8029):145–145. doi: 10.1016/s0140-6736(77)90161-1. [DOI] [PubMed] [Google Scholar]
  15. Wüst J., Wilkins T. D. Effect of clavulanic Acid on anaerobic bacteria resistant to Beta-lactam antibiotics. Antimicrob Agents Chemother. 1978 Jan;13(1):130–133. doi: 10.1128/aac.13.1.130. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Zimmermann W., Rosselet A. Function of the outer membrane of Escherichia coli as a permeability barrier to beta-lactam antibiotics. Antimicrob Agents Chemother. 1977 Sep;12(3):368–372. doi: 10.1128/aac.12.3.368. [DOI] [PMC free article] [PubMed] [Google Scholar]

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