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. 1979 Mar;15(3):436–439. doi: 10.1128/aac.15.3.436

Purification of a Streptococcal Bacteriocin (Viridin B) and Its Separation from Alpha-Hemolysin

Lynn D Apelgren 1, Adnan S Dajani 1
PMCID: PMC352678  PMID: 464570

Abstract

Viridin B, a bacteriocin produced by Streptococcus mitis, copurified with the alpha-hemolysin after ammonium sulfate precipitation and gel filtration on Sephadex G-200. The bacteriocin and hemolysin were separated in some instances by ion-exchange chromatography on diethylaminoethyl-Sephadex A-50, but the two substances were shown to be distinct after polyacrylamide gel electrophoresis. Attempts at recovery of nonhemolytic or nonbacteriocinogenic mutants were unsuccessful after exposure to mutagenic agents. The molecular weight of viridin B was determined to be approximately 87,000.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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