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Whole-genome sequencing (WGS) and mapping of yc20. (A) Summary of yc20 WGS. (B) The yc20 mutation was mapped to the toca-1 region. Each peak represents a cluster of G-to-A or C-to-T mutations identified by WGS characteristic of EMS mutagenesis. Since yc20 was backcrossed after mutagenesis, it is expected that the remaining EMS-induced mutations would be linked to yc20 on chromosome X.
