Abstract
A total of 24 strains of the Mycobacterium fortuitum complex were tested for susceptibility to antimicrobial agents by the disk diffusion and agar dilution techniques. By comparing zones of inhibition obtained with the disk diffusion technique with results of minimal inhibitory concentration determinations, it was shown that disk diffusion results could predict in vitro susceptibility to selected antimicrobial agents. All of 17 strains of M. fortuitum were susceptible to ≤1 μg of amikacin per ml. The corresponding average zone of inhibition around a 10-μg amikacin disk was 37 mm. Seven M. chelonei strains were more resistant to amikacin, with minimal inhibitory concentrations ranging from 1 to 32 μg/ml, and the corresponding average zone size was 21 mm. Susceptibility of both M. fortuitum and M. chelonei to tetracycline was variable and none of the M. chelonei strains was inhibited by polymyxin B, whereas M. fortuitum strains consistently had zones of inhibition around the polymyxin disk. It appears that identification to species of the M. fortuitum complex may be of importance with regard to antibiotic susceptibility. Separation of M. fortuitum and M. chelonei was readily accomplished in the present study by the nitrate reduction and 3-day arylsulfatase tests.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
- Booth J. E., Jacobson J. A., Kurrus T. A., Edwards T. W. Infection of prosthetic arthroplasty by Mycobacterium fortuitum. Two case reports. J Bone Joint Surg Am. 1979 Mar;61(2):300–302. [PubMed] [Google Scholar]
- Dalovisio J. R., Pankey G. A. In vitro susceptiiblity of Mycobacterium fortuitum and Mycobacterium chelonei to amikacin. J Infect Dis. 1978 Mar;137(3):318–321. doi: 10.1093/infdis/137.3.318. [DOI] [PubMed] [Google Scholar]
- Dreisin R. B., Scoggin C., Davidson P. T. The pathogenicity of Mycobacterium fortuitum and Mycobacterium chelonei in man: a report of seven cases. Tubercle. 1976 Mar;57(1):49–57. doi: 10.1016/0041-3879(76)90017-9. [DOI] [PubMed] [Google Scholar]
- Hand W. L., Sanford J. P. Mycobacterium fortuitum--a human pathogen. Ann Intern Med. 1970 Dec;73(6):971–977. doi: 10.7326/0003-4819-73-6-971. [DOI] [PubMed] [Google Scholar]
- Offer R. C., Karlson A. G., Spittell J. A., Jr Infection caused by Mycobacterium fortuitum. Mayo Clin Proc. 1971 Nov;46(11):747–750. [PubMed] [Google Scholar]
- SHOCKET E., DROSD R. E., TATE C. F., Jr GRANULOMA OF THE SKIN DUE TO MYCOBACTERIUM FORTUITUM. South Med J. 1964 Nov;57:1352–1356. doi: 10.1097/00007611-196411000-00019. [DOI] [PubMed] [Google Scholar]
- Sanders W. E., Jr, Hartwig E. C., Schneider N. J., Cacciatore R., Valdez H. Susceptibility of organisms in the Mycobacterium fortuitum complex to antituberculous and other antimicrobial agents. Antimicrob Agents Chemother. 1977 Aug;12(2):295–297. doi: 10.1128/aac.12.2.295. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wayne L. G., Doubek J. R. Diagnostic key to mycobacteria encountered in clinical laboratories. Appl Microbiol. 1968 Jun;16(6):925–931. doi: 10.1128/am.16.6.925-931.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]