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. 1979 Jul;16(1):77–80. doi: 10.1128/aac.16.1.77

In Vitro Model Simulating the Form of Exposure of Bacteria to Antimicrobial Drugs Encountered in Infection

Mohammad J S Al-Asadi 1, David Greenwood 1, Francis O'Grady 1
PMCID: PMC352792  PMID: 383014

Abstract

A new model, which is designed to investigate the in vitro activity of antibiotics as a function of different concentration-time curves, is described. The antibiotic is allowed to diffuse through a membrane into a bacterial culture until a peak level is reached; the antibiotic is then removed by flow-assisted back diffusion. With this arrangement it is possible to expose bacteria to a changing concentration of drug while maintaining a constant volume of bacterial culture. Preliminary studies were carried out to investigate the response of a strain of Escherichia coli to gentamicin. The results indicate that bacteria surviving exposure to concentrations of gentamicin similar to those obtained during therapy may exhibit an increase in resistance to the antibiotic.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bodey G. P., Middleman E., Umsawadi T., Rodriguez V. Infections in cancer patients. Results with gentamicin sulfate therapy. Cancer. 1972 Jun;29(6):1697–1701. doi: 10.1002/1097-0142(197206)29:6<1697::aid-cncr2820290638>3.0.co;2-k. [DOI] [PubMed] [Google Scholar]
  2. Grasso S., Meinardi G., de Carneri I., Tamassia V. New in vitro model to study the effect of antibiotic concentration and rate of elimination on antibacterial activity. Antimicrob Agents Chemother. 1978 Apr;13(4):570–576. doi: 10.1128/aac.13.4.570. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Greenwood D., O'Grady F. An in vitro model of the urinary bladder. J Antimicrob Chemother. 1978 Mar;4(2):113–120. doi: 10.1093/jac/4.2.113. [DOI] [PubMed] [Google Scholar]
  4. Mackintosh I. P., O'Grady F., Greenwood D., Watson B. W., Crichton T. C., Piper R., Ferrer A. A twelve channel bacterial growth monitoring system. Biomed Eng. 1973 Dec;8(12):514–passim. [PubMed] [Google Scholar]
  5. Mawer S. L., Greenwood D. Specific and non-specific resistance to aminoglycosides in Escherichia coli. J Clin Pathol. 1978 Jan;31(1):12–15. doi: 10.1136/jcp.31.1.12. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Nishida M., Murakawa T., Kamimura T., Okada N. Bactericidal activity of cephalosporins in an in vitro model simulating serum levels. Antimicrob Agents Chemother. 1978 Jul;14(1):6–12. doi: 10.1128/aac.14.1.6. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. O'Grady F. Animal models in the assessment of antimicrobial agents. J Antimicrob Chemother. 1976 Mar;2(1):1–3. doi: 10.1093/jac/2.1.1-a. [DOI] [PubMed] [Google Scholar]
  8. Seal D. V., Strangeways J. E. Aminoglycoside resistance due to mutation. Lancet. 1977 Apr 16;1(8016):856–857. doi: 10.1016/s0140-6736(77)92805-7. [DOI] [PubMed] [Google Scholar]

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