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. 1979 Dec;16(6):731–735. doi: 10.1128/aac.16.6.731

In vitro antibacterial activity of cefoperazone (T-1551), a new semisynthetic cephalosporin.

N Matsubara, S Minami, T Muraoka, I Saikawa, S Mitsuhashi
PMCID: PMC352944  PMID: 316988

Abstract

Cefoperazone, a new semisynthetic cephalosporin, has a broad spectrum of antibacterial activity. It is as active as cefazolin and cefamandole against gram-positive bacteria and is more active than cefazolin and cefamandole against such gram-negative bacilli as Escherichia coli, Klebsiella pneumoniae, Proteus species, Pseudomonas aeruginosa, Citrobacter freundii, Enterobacter cloacae, and Serratia marcescens. The superiority of cefoperazone over cefazolin and cefamandole with respect to activity against P. aeruginosa by more than 200-fold was especially remarkable. As with other beta-lactam antibiotics, there was only a small spread between the minimum inhibitory concentrations and the minimum bactericidal concentrations of cefoperazone and a significant decrease in activity with an increase in inoculum size. Activity was not altered significantly by the addition of human serum to the test medium. Cefoperazone is relatively stable to hydrolysis to beta-lactamases produced by gram-negative bacteria. Relative rates of hydrolysis of cefoperazone by cephalosporinases are 7.0 to 0.01, with reference to cephaloridine hydrolysis (base, 100). Cefoperazone is also more stable than penicillin G and cephaloridine to various types of penicillinases.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Acred P., Brown D. M., Knudsen E. T., Rolinson G. N., Sutherland R. New semi-synthetic penicillin active against Pseudomonas pyocyanea. Nature. 1967 Jul 1;215(5096):25–30. doi: 10.1038/215025a0. [DOI] [PubMed] [Google Scholar]
  2. Eykyn S., Jenkins C., King A., Phillips I. Antibacterial activity of cefamandole, a new cephalosporin antibiotic, compared with that of cephaloridine, cephalothin, and cephalexin. Antimicrob Agents Chemother. 1973 Jun;3(6):657–661. doi: 10.1128/aac.3.6.657. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  4. Motley M., Shadomy S. In vitro studies with cefazolin. Antimicrob Agents Chemother. 1974 Dec;6(6):856–861. doi: 10.1128/aac.6.6.856. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Newsom S. W., Sykes R. B., Richmond M. H. Detection of a beta-lactamase markedly active against carbenicillin in a strain of Pseudomonas aeruginosa. J Bacteriol. 1970 Mar;101(3):1079–1080. doi: 10.1128/jb.101.3.1079-1080.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Reller L. B., Karney W. W., Beaty H. N., Holmes K. K., Turck M. Evaluation of cefazolin, a new cephalosporin antibiotic. Antimicrob Agents Chemother. 1973 Apr;3(4):488–497. doi: 10.1128/aac.3.4.488. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Samuni A. A direct spectrophotometric assay and determination of Michaelis constants for the beta-lactamase reaction. Anal Biochem. 1975 Jan;63(1):17–26. doi: 10.1016/0003-2697(75)90185-2. [DOI] [PubMed] [Google Scholar]
  8. Sawada Y., Yaginuma S., Tai M., Iyobe S., Mitsuhashi S. Plasmid-mediated penicillin beta-lactamases in Pseudomonas aeruginosa. Antimicrob Agents Chemother. 1976 Jan;9(1):55–60. doi: 10.1128/aac.9.1.55. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Sawai T., Mitsuhashi S., Yamagishi S. Drug resistance of enteric bacteria. XIV. Comparison of beta-lactamases in gram-negative rod bacteria resistant to alpha-aminobenzylpenicillin. Jpn J Microbiol. 1968 Dec;12(4):423–434. doi: 10.1111/j.1348-0421.1968.tb00415.x. [DOI] [PubMed] [Google Scholar]
  10. Waley S. G. A spectrophotometric assay of beta-lactamase action on penicillins. Biochem J. 1974 Jun;139(3):789–790. doi: 10.1042/bj1390789. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Wick W. E., Preston D. A. Biological properties of three 3-heterocyclic-thiomethyl cephalosporin antibiotics. Antimicrob Agents Chemother. 1972 Mar;1(3):221–234. doi: 10.1128/aac.1.3.221. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Yaginuma S., Sawai T., Ono H., Yamagishi S., Mitsuhashi S. Biochemical properties of a cephalosporin beta-lactamase from Pseudomonas aeruginosa. Jpn J Microbiol. 1973 Mar;17(2):141–149. doi: 10.1111/j.1348-0421.1973.tb00718.x. [DOI] [PubMed] [Google Scholar]
  13. Yamagishi S., O'Hara K., Sawai T., Mitsuhashi S. The purification and properties of penicillin beta-lactamases mediated by transmissible R factors in Escherichia coli. J Biochem. 1969 Jul;66(1):11–20. doi: 10.1093/oxfordjournals.jbchem.a129111. [DOI] [PubMed] [Google Scholar]

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