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. 1979 Jan;29(1):385–389. doi: 10.1128/jvi.29.1.385-389.1979

Subcellular distribution of newly synthesized virus-specific polypeptides in Moloney murine leukemia virus infected cells.

G Shanmugam
PMCID: PMC353137  PMID: 430597

Abstract

Immune precipitation analysis of pulse-labeled proteins present in subcellular fractions of mouse embryo cells infected with Moloney murine leukemia virus showed the presence of anti-gp70 serum-precipitable viral envelope gene products mainly in the microsomal fractions of these cells. In contrast, anti-p30 serum-specific gag (group specific antigen) gene products were found to be distributed in similar amounts in both the microsomal and postmicrosomal supernatant fractions of pulse-labeled cells.

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Selected References

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  1. Baglioni C., Bleiberg I., Zauderer M. Assembly of membrane-bound polyribosomes. Nat New Biol. 1971 Jul 7;232(27):8–12. doi: 10.1038/newbio232008a0. [DOI] [PubMed] [Google Scholar]
  2. Fan H., Baltimore D. RNA metabolism of murine leukemia virus: detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA. J Mol Biol. 1973 Oct 15;80(1):93–117. doi: 10.1016/0022-2836(73)90235-0. [DOI] [PubMed] [Google Scholar]
  3. Gielkens A. L., Salden M. H., Bloemendal H. Virus-specific messenger RNA on free and membrane-bound polyribosomes from cells infected with Rauscher leukemia virus. Proc Natl Acad Sci U S A. 1974 Apr;71(4):1093–1097. doi: 10.1073/pnas.71.4.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gielkens A. L., Van Zaane D., Bloemers H. P., Bloemendal H. Synthesis of Rauscher murine leukemia virus-specific polypeptides in vitro. Proc Natl Acad Sci U S A. 1976 Feb;73(2):356–360. doi: 10.1073/pnas.73.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Harter M. L., Shanmugam G., Wold W. S., Green M. Detection of adenovirus type 2-induced early polypeptides using cycloheximide pretreatment to enhance viral protein synthesis. J Virol. 1976 Jul;19(1):232–242. doi: 10.1128/jvi.19.1.232-242.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hayman M. Synthesis and processing of avian sarcoma virus glycoproteins. Virology. 1978 Apr;85(2):475–486. doi: 10.1016/0042-6822(78)90454-3. [DOI] [PubMed] [Google Scholar]
  7. Kerr I. M., Olshevsky U., Lodish H. F., Baltimore D. Translation of murine leukemia virus RNA in cell-free systems from animal cells. J Virol. 1976 May;18(2):627–635. doi: 10.1128/jvi.18.2.627-635.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kimmel C. B. On the RNA in cultured myeloma cells producing immunoglobulin. Biochim Biophys Acta. 1969 Jun 17;182(2):361–374. doi: 10.1016/0005-2787(69)90187-7. [DOI] [PubMed] [Google Scholar]
  9. Leis J. P., McGinnis J., Green R. W. Rous sarcoma virus p19 binds to specific double-stranded regions of viral RNA: effect of p19 on cleavage of viral RNA by RNase III. Virology. 1978 Jan;84(1):87–98. doi: 10.1016/0042-6822(78)90220-9. [DOI] [PubMed] [Google Scholar]
  10. Lueders K. K. In vitro synthesis of A-particle structual protein by membrane-bound polyribosomes. J Virol. 1976 Jun;18(3):1088–1094. doi: 10.1128/jvi.18.3.1088-1094.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Moelling K., Hayami M. Analysis of precursors to the envelope glycoproteins of avian RNA tumor viruses in chicken and quail cells. J Virol. 1977 Jun;22(3):598–607. doi: 10.1128/jvi.22.3.598-607.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pawson T., Harvey R., Smith A. E. The size of Rous sarcoma virus mRNAs active in cell-free translation. Nature. 1977 Aug 4;268(5619):416–420. doi: 10.1038/268416a0. [DOI] [PubMed] [Google Scholar]
  13. Pinter A., Fleissner E. The presence of disulfide-linked gp70-p15(E) complexes in AKR murine leukemia virus. Virology. 1977 Dec;83(2):417–422. doi: 10.1016/0042-6822(77)90187-8. [DOI] [PubMed] [Google Scholar]
  14. Shanmugam G., Bhaduri S., Green M. The virus-specific RNA species in free and membrane-bound polyribosomes of transformed cells replicating murine sarcoma-leukemia viruses. Biochem Biophys Res Commun. 1974 Feb 4;56(3):697–702. doi: 10.1016/0006-291x(74)90661-5. [DOI] [PubMed] [Google Scholar]
  15. Shanmugam G. Endonucleolytic cleavage of murine leukemia virus 35S RNA by microsome-associated nuclease. Biochem Biophys Res Commun. 1976 Jun 7;70(3):818–823. doi: 10.1016/0006-291x(76)90665-3. [DOI] [PubMed] [Google Scholar]
  16. Shanmugam G. Rapid metabolism of moloney leukemia virus precursor polypeptides in virus infected Swiss mouse embryo cells. Biochem Biophys Res Commun. 1977 Sep 23;78(2):517–524. doi: 10.1016/0006-291x(77)90209-1. [DOI] [PubMed] [Google Scholar]
  17. Shanmugam G., Vecchio G., Attardi D., Green M. Immunological studies on viral polypeptide synthesis in cells replicating murine sarcoma-leukemia virus. J Virol. 1972 Sep;10(3):447–455. doi: 10.1128/jvi.10.3.447-455.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Stacey D. W., Allfrey V. G., Hanafusa H. Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1614–1618. doi: 10.1073/pnas.74.4.1614. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Tsuchida N., Robin M. S., Green M. Viral RNA subunits in cells transformed by RNA tumor viruses. Science. 1972 Jun 30;176(4042):1418–1420. doi: 10.1126/science.176.4042.1418. [DOI] [PubMed] [Google Scholar]
  20. Van Zaane D., Dekker-Michielsen J. A., Bloemers H. P. Virus-specific precursor polypeptides in cells infected with Rauscher leukemia virus: synthesis, identification, and processing. Virology. 1976 Nov;75(1):113–129. doi: 10.1016/0042-6822(76)90011-8. [DOI] [PubMed] [Google Scholar]
  21. Vassart G. Specific synthesis of thyroglobulin on membrane bound thyroid ribosomes. FEBS Lett. 1972 Apr 15;22(1):53–56. doi: 10.1016/0014-5793(72)80217-5. [DOI] [PubMed] [Google Scholar]
  22. Vecchio G., Tsuchida N., Shanmugam G., Green M. Virus-specific messenger RNA and nascent polypeptides in polyribosomes of cells replicating murine sarcoma-leukemia viruses. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2064–2068. doi: 10.1073/pnas.70.7.2064. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Vogt V. M., Eisenman R., Diggelmann H. Generation of avian myeloblastosis virus structural proteins by proteolytic cleavage of a precursor polypeptide. J Mol Biol. 1975 Aug 15;96(3):471–493. doi: 10.1016/0022-2836(75)90174-6. [DOI] [PubMed] [Google Scholar]
  24. Wirth D. F., Katz F., Small B., Lodish H. F. How a single Sindbis virus mRNA directs the synthesis of one soluble protein and two integral membrane glycoproteins. Cell. 1977 Feb;10(2):253–263. doi: 10.1016/0092-8674(77)90219-7. [DOI] [PubMed] [Google Scholar]
  25. Zaane D. V., Gielkens A. L., Hesselink W. G., Bloemers H. P. Identification of Rauscher murine leukemia virus-specific mRNAs for the synthesis of gag- and env-gene products. Proc Natl Acad Sci U S A. 1977 May;74(5):1855–1859. doi: 10.1073/pnas.74.5.1855. [DOI] [PMC free article] [PubMed] [Google Scholar]

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