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. 1979 Feb;29(2):540–545. doi: 10.1128/jvi.29.2.540-545.1979

Order of assembly of the lower collar and the tail proteins of Bacillus subtilis bacteriophage phi 29.

A Camacho, F Jiménez, E Viñuela, M Salas
PMCID: PMC353188  PMID: 107325

Abstract

Extracts obtained after restrictive infection of Bacillus subtilis with mutants in cistron 11 of bacteriophage phi 29 are complemented in vitro by extract donors of the lower collar protein (p11). Purified 11- heads, containing the major capsid protein (p8), the fiber protein (p8.5), the upper collar protein (p10), and the virus DNA, can be also complemented in vitro to produce infective virus. This result suggests that 11- heads are intermediates in phage phi 29 morphogenesis. The order of assembly of the lower collar protein p11 and the tail protein p9 was determined in vitro in two complementation steps. The results obtained indicate that the lower collar protein is assembled before the tail protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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