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. 1979 Feb;29(2):624–632. doi: 10.1128/jvi.29.2.624-632.1979

Rosettes from Friend leukemia virus envelope: preparation and physicochemical and partial biological characterization.

J Schneider, H Schwarz, G Hunsmann
PMCID: PMC353195  PMID: 85724

Abstract

Rosette-shaped particles mainly containing gp85 were isolated from Friend leukemia virus. The isolation procedure comprised lysis of the virion by Triton X-100, affinity chromatography on concanavalin A-Sepharose, and velocity sedimentation. The rosettes displayed a mean sedimentation constant of 32S and a buoyant density of 1.21 g/ml. They contained 1% Triton X-100 and about 2% phospholipid. gp85 was identified by polyacrylamide electrophoresis, staining with periodic acid-Schiff reagent, and immunoprecipitation with antisera against Friend leukemia virus gp71 and p15(E). Rosettes completely blocked the cytotoxicity of the gp71 antiserum. The ability to hemagglutinate was inhibited by antibodies to gp71.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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