Abstract
The technique of competition hybridization in DNA excess was used to determine the intracellular distribution of RNA genomes of Rauscher murine leukemia virus. An examination of subcellular RNA fractions revealed that 59% of intracellular viral RNA genomes were associated with the nuclear-enriched fraction, 41% with the cytoplasmic fraction, and 18% with the polysomal-enriched fraction. Also, an analysis of total cellular RNA disclosed that 20% of intracellular viral RNA genomes were polyadenylated and 80% were not polyadenylated.
Full text
PDF






Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Colcher D., Drohan W., Schlom Mason-Pfizer virus RNA genome: relationship to the RNA of morphologically similar isolates and other oncornaviruses. J Virol. 1976 Mar;17(3):705–712. doi: 10.1128/jvi.17.3.705-712.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Conely A. J., Velicer L. F. Analysis of cytoplasmic RNA and polyribosmomes from feline leukemia virus-infected cells. J Virol. 1978 Mar;25(3):750–763. doi: 10.1128/jvi.25.3.750-763.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis A. R., Nayak D. P. Expression of endogenous retroviral genes in leukemic guinea pig cells. J Virol. 1977 Aug;23(2):263–271. doi: 10.1128/jvi.23.2.263-271.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Davis A. R., Nayak D. P., Lofgren J. Induction of endogenous guinea pig retrovirus by 5-bromodeoxyuridine: amplification of virus-specific RNA. J Virol. 1978 Jun;26(3):603–614. doi: 10.1128/jvi.26.3.603-614.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- East J. L., Knesek J. E., Chan J. C., Dmochowski L. Quantitative nucleotide sequence relationships of mammalian RNA tumor viruses. J Virol. 1975 Jun;15(6):1396–1408. doi: 10.1128/jvi.15.6.1396-1408.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fan H., Baltimore D. RNA metabolism of murine leukemia virus: detection of virus-specific RNA sequences in infected and uninfected cells and identification of virus-specific messenger RNA. J Mol Biol. 1973 Oct 15;80(1):93–117. doi: 10.1016/0022-2836(73)90235-0. [DOI] [PubMed] [Google Scholar]
- Fan H., Besmer P. RNA metabolism of murine leukemia virus II. Endogenous virus-specific RNA in the uninfected BALB/c cell line JLS-V9. J Virol. 1975 Apr;15(4):836–842. doi: 10.1128/jvi.15.4.836-842.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fidanián H. M., Drohán W. N., Baluda M. A. RNA of simian sarcoma-associated virus type 1 produced in human tumor cells. J Virol. 1975 Mar;15(3):449–457. doi: 10.1128/jvi.15.3.449-457.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Getz M. J., Altenburg L. C., Saunders G. F. The use of RNA labeled in vitro with iodine-125 in molecular hybridization experiments. Biochim Biophys Acta. 1972 Dec 22;287(3):485–494. doi: 10.1016/0005-2787(72)90293-6. [DOI] [PubMed] [Google Scholar]
- Gielkens A. L., Van Zaane D., Bloemers H. P., Bloemendal H. Synthesis of Rauscher murine leukemia virus-specific polypeptides in vitro. Proc Natl Acad Sci U S A. 1976 Feb;73(2):356–360. doi: 10.1073/pnas.73.2.356. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Haseltine W. A., Baltimore D. Size of murine RNA tumor virus-specific nuclear RNA molecules. J Virol. 1976 Aug;19(2):331–337. doi: 10.1128/jvi.19.2.331-337.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayward W. S., Hanafusa H. Detection of avian tumor virus RNA in uninfected chicken embryo cells. J Virol. 1973 Feb;11(2):157–167. doi: 10.1128/jvi.11.2.157-167.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hayward W. S. Size and genetic content of viral RNAs in avian oncovirus-infected cells. J Virol. 1977 Oct;24(1):47–63. doi: 10.1128/jvi.24.1.47-63.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Krzyzek R. A., Lau A. F., Vogt P. K., Faras A. J. Quantitation and localization of Rous sarcoma virus-specific RNA in transformed and revertant field vole cells. J Virol. 1978 Feb;25(2):518–526. doi: 10.1128/jvi.25.2.518-526.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Leong J. A., Garapin A. C., Jackson N., Fanshier L., Levinson W., Bishop J. M. Virus-specific ribonucleic acid in cells producing rous sarcoma virus: detection and characterization. J Virol. 1972 Jun;9(6):891–902. doi: 10.1128/jvi.9.6.891-902.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michalides R., Schlom J., Dahlberg J., Perk K. Biochemical properties of the bromodeoxyuridine-induced guinea pig virus. J Virol. 1975 Oct;16(4):1039–1050. doi: 10.1128/jvi.16.4.1039-1050.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Michalides R., Schlom J. Relationship in nucleic acid sequences between mouse mammary tumor virus variants. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4635–4639. doi: 10.1073/pnas.72.11.4635. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neiman P. E., Wright S. E., McMillin C., MacDonnell D. Nucleotide sequence relationships of avian RNA tumor viruses: measurement of the deletion in a transformation-defective mutant of Rous sarcoma virus. J Virol. 1974 Apr;13(4):837–846. doi: 10.1128/jvi.13.4.837-846.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Okabe H., Gilden R. V., Hatanaka M. RD 114 virus-specific sequences in feline cellular RNA: detection and characterization. J Virol. 1973 Nov;12(5):984–994. doi: 10.1128/jvi.12.5.984-994.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Parsons J. T., Lewis P., Dierks P. Purification of virus-specific RNA from chicken cells infected with avian sarcoma virus: identification of genome-length and subgenome-leghth viral RNAs. J Virol. 1978 Jul;27(1):227–238. doi: 10.1128/jvi.27.1.227-238.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Ringold G. M., Blair P. B., Bishop J. M., Varmus H. E. Nucleotide sequence homologies among mouse mammary tumor viruses. Virology. 1976 Apr;70(2):550–553. doi: 10.1016/0042-6822(76)90297-x. [DOI] [PubMed] [Google Scholar]
- Schincariol A. L., Joklik W. K. Early synthesis of virus-specific RNA and DNA in cells rapidly transformed with Rous sarcoma virus. Virology. 1973 Dec;56(2):532–548. doi: 10.1016/0042-6822(73)90056-1. [DOI] [PubMed] [Google Scholar]
- Scolnick E. M., Benveniste R., Parks W. P. Purification by oligo(dT)-cellulose of viral-specific RNA from sarcoma virus-transformed mammalian nonproducer cells. J Virol. 1973 Apr;11(4):600–602. doi: 10.1128/jvi.11.4.600-602.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Singer R. H., Penman S. Messenger RNA in HeLa cells: kinetics of formation and decay. J Mol Biol. 1973 Aug 5;78(2):321–334. doi: 10.1016/0022-2836(73)90119-8. [DOI] [PubMed] [Google Scholar]
- Syrewicz J. J., Naso R. B., Wang C. S., Arlinghaus R. B. Purification of large amounts of murine ribonucleic acid tumor viruses produced in roller bottle cultures. Appl Microbiol. 1972 Sep;24(3):488–494. doi: 10.1128/am.24.3.488-494.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Tsuchida N., Green M. Intracellular and virion 35 S RNA species of murine sarcoma and leukemia viruses. Virology. 1974 May;59(1):258–265. doi: 10.1016/0042-6822(74)90221-9. [DOI] [PubMed] [Google Scholar]
- Weiss S. R., Varmus H. E., Bishop J. M. The size and genetic composition of virus-specific RNAs in the cytoplasm of cells producing avian sarcoma-leukosis viruses. Cell. 1977 Dec;12(4):983–992. doi: 10.1016/0092-8674(77)90163-5. [DOI] [PubMed] [Google Scholar]