Abstract
At both early (3 h) and late (18 h) times after infection of KB cells with adenovirus 2, more than 90% of parental nuclear viral genomes exist as complexes which contain terminally linked proteins. Density shift experiments employing 5-bromo-2'-deoxyuridine indicate that these parental DNA molecules remain complexed with terminal proteins after DNA replication. The persistent linkage of proteins to the termini of intranuclear viral DNA suggests that these proteins have an essential role in adenovirus replication.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Chinnadurai G., Chinnadurai S., Green M. Enhanced infectivity of adenovirus type 2 DNA and a DNA-protein complex. J Virol. 1978 Apr;26(1):195–199. doi: 10.1128/jvi.26.1.195-199.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig E. A. Analysis of early adenovirus 2 RNA using Eco R-R1 viral DNA fragments. J Virol. 1975 May;15(5):1202–1213. doi: 10.1128/jvi.15.5.1202-1213.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Craig E. A., Raskas H. J. Nuclear transcripts larger than the cytoplasmic mRNAs are specified by segments of the adenovirus genome coding for early functions. Cell. 1976 Jun;8(2):205–213. doi: 10.1016/0092-8674(76)90004-0. [DOI] [PubMed] [Google Scholar]
- Craig E. A., Raskas H. J. Two classes of cytoplasmic viral RNA synthesized early in productive infection with adenovirus 2. J Virol. 1974 Oct;14(4):751–757. doi: 10.1128/jvi.14.4.751-757.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Estes M. K. Characterization of DNA-protein complexes from simian adenovirus SA7. J Virol. 1978 Mar;25(3):917–922. doi: 10.1128/jvi.25.3.917-922.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McGrogan M., Raskas H. J. Species identification and genome mapping of cytoplasmic adenovirus type 2 RNAs synthesized late in infection. J Virol. 1977 Aug;23(2):240–249. doi: 10.1128/jvi.23.2.240-249.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mulder C., Arrand J. R., Delius H., Keller W., Pettersson U., Roberts R. J., Sharp P. A. Cleavage maps of DNA from adenovirus types 2 and 5 by restriction endonucleases EcoRI and HpaI. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):397–400. doi: 10.1101/sqb.1974.039.01.051. [DOI] [PubMed] [Google Scholar]
- Padmanabhan R., Padmanabhan R. V. Specific interaction of a protein(s) at or near the termini of adenovirus 2 DNA. Biochem Biophys Res Commun. 1977 Apr 25;75(4):955–964. doi: 10.1016/0006-291x(77)91475-9. [DOI] [PubMed] [Google Scholar]
- Rekosh D. M., Russell W. C., Bellet A. J., Robinson A. J. Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977 Jun;11(2):283–295. doi: 10.1016/0092-8674(77)90045-9. [DOI] [PubMed] [Google Scholar]
- Robinson A. J., Bellett J. D. A circular DNA-protein complex adenoviruses and its possible role in DNA replication. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):523–531. doi: 10.1101/sqb.1974.039.01.064. [DOI] [PubMed] [Google Scholar]
- Robinson A. J., Younghusband H. B., Bellett A. J. A circula DNA-protein complex from adenoviruses. Virology. 1973 Nov;56(1):54–69. doi: 10.1016/0042-6822(73)90287-0. [DOI] [PubMed] [Google Scholar]
- Sharp P. A., Moore C., Haverty J. L. The infectivity of adenovirus 5 DNA-protein complex. Virology. 1976 Dec;75(2):442–456. doi: 10.1016/0042-6822(76)90042-8. [DOI] [PubMed] [Google Scholar]
- Sharp P. A., Sugden B., Sambrook J. Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis. Biochemistry. 1973 Jul 31;12(16):3055–3063. doi: 10.1021/bi00740a018. [DOI] [PubMed] [Google Scholar]
- Vlak J. M., Rozijn T. H., Spies F. Replication of adenovirus type 5 DNA in KB cells: localization and fate of parental DNA during replication. Virology. 1976 Jul 1;72(1):99–109. doi: 10.1016/0042-6822(76)90315-9. [DOI] [PubMed] [Google Scholar]