Figure 3.

Ethanol-induced second-order conditioning in infant rats derived from pregnant rats that were given 2.0 g/kg ethanol or vehicle (water) intragastrically (PE and PV groups, respectively) on gestational days 17 to 20 or were untreated (UT group), with the percent time spent on the rough CS₂ texture (sandpaper) during the test as the dependent variable. During conditioning on PD14, the animals were given ethanol (2.0 or 3.0 g/kg, i.g.) and stimulated 30–45 min post-administration with intraoral pulses of water (CS₁; paired group) or experienced both stimuli separated by 120 min (unpaired groups). The next day, the animals experienced CS₁–CS₂ pairings and were then tested for CS₂ preference. The upper panel depicts the percent time spent on sandpaper as a function of prenatal treatment and treatment during conditioning. The lower panel depicts these data disaggregated by ethanol dose given at conditioning. To facilitate data visualization, the data were collapsed across sex. The latter factor did not affect tactile preference scores or significantly interact with the remaining factors. Asterisks in the upper panel (*) indicate significant differences between a paired group and its corresponding unpaired control (p < 0.05). Vertical bars indicate the SEM.