Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1979 Mar;29(3):1006–1013. doi: 10.1128/jvi.29.3.1006-1013.1979

RNA-directed DNA polymerase from particles released by normal goose cells.

G Bauer, H M Temin
PMCID: PMC353261  PMID: 87517

Abstract

Cells from a goose embryo were shown to release particle-associated RNA-directed DNA polymerase and RNase H activities that required the presence of Nonidet P-40 for detection. The particles were not infectious and did not have endogenous DNA synthesis. The goose particle DNA polymerase was related to the DNA polymerase of spleen necrosis virus with respect to size and was inhibited by immunoglobulin G to spleen necrosis virus DNA polymerase. However, goose cells producing DNA polymerase-containing particles did not contain reticuloendotheliosis virus-related nucleotide sequences in their DNA.

Full text

PDF
1006

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bauer G., Friis R. R., Mattersberger H., Hofschneider P. H. Controlled release of particle-associated RNA-dependent DNA polymerase by primary chick embryo cell cultures. Exp Cell Res. 1978 Dec;117(2):383–392. doi: 10.1016/0014-4827(78)90151-9. [DOI] [PubMed] [Google Scholar]
  3. Bauer G., Hofschneider P. H. An RNA-dependent DNA polymerase, different from the known viral reverse transcriptases, in the chicken system. Proc Natl Acad Sci U S A. 1976 Sep;73(9):3025–3029. doi: 10.1073/pnas.73.9.3025. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bauer G., Jilek G., Hofschneider P. H. Purification and further characterization of an RNA-dependent DNA polymerase from the allantoic fluid of leukosis-virus-free chicken eggs. Eur J Biochem. 1977 Oct 3;79(2):345–354. doi: 10.1111/j.1432-1033.1977.tb11815.x. [DOI] [PubMed] [Google Scholar]
  5. Kang C. Y., Temin H. M. Endogenous RNA-directed DNA polymerase activity in uninfected chicken embryos. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1550–1554. doi: 10.1073/pnas.69.6.1550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kang C. Y., Temin H. M. Reticuloendotheliosis virus nucleic acid sequences in cellular DNA. J Virol. 1974 Nov;14(5):1179–1188. doi: 10.1128/jvi.14.5.1179-1188.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Marcus S. L., Modak M. J., Cavalieri L. F. Purification of avian myeloblastosis virus DNA polymerase by affinity chromatography on polycytidylate-agarose. J Virol. 1974 Oct;14(4):853–859. doi: 10.1128/jvi.14.4.853-859.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mizutani A., Temin H. M. Purification and properties of spleen necrosis virus DNA polymerase. J Virol. 1975 Oct;16(4):797–806. doi: 10.1128/jvi.16.4.797-806.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Mölling K., Bolognesi D. P., Bauer H., Büsen W., Plassmann H. W., Hausen P. Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids. Nat New Biol. 1971 Dec 22;234(51):240–243. doi: 10.1038/newbio234240a0. [DOI] [PubMed] [Google Scholar]
  12. Schaffner W., Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal Biochem. 1973 Dec;56(2):502–514. doi: 10.1016/0003-2697(73)90217-0. [DOI] [PubMed] [Google Scholar]
  13. Schmidt J., Pragnell I. B., Weimann B. J. DNA polymerases from intracisternal A-type particles on the mouse plasmacytoma MPC 11. Eur J Biochem. 1977 Mar 1;73(2):493–497. doi: 10.1111/j.1432-1033.1977.tb11342.x. [DOI] [PubMed] [Google Scholar]
  14. Taylor J. M., Illmensee R., Summers J. Efficeint transcription of RNA into DNA by avian sarcoma virus polymerase. Biochim Biophys Acta. 1976 Sep 6;442(3):324–330. doi: 10.1016/0005-2787(76)90307-5. [DOI] [PubMed] [Google Scholar]
  15. Watson K. E., Nowinski R. C., Yaniv A., Spiegelman S. Serological analysis of the deoxyribonucleic acid polymerase of avian oncornaviruses. I. Preparation and characterization of monospecific antiserum with purified deoxyribonucleic acid polymerase. J Virol. 1972 Nov;10(5):951–958. doi: 10.1128/jvi.10.5.951-958.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Yang S. S., Wivel N. A. Characterization of an endogenous RNA-dependent DNA polymerase associated with murine intracisternal A particles. J Virol. 1974 Mar;13(3):712–720. doi: 10.1128/jvi.13.3.712-720.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES