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. 1979 Mar;29(3):1118–1130. doi: 10.1128/jvi.29.3.1118-1130.1979

Unusual Baculovirus of the Parasitoid Wasp Apanteles melanoscelus: Isolation and Preliminary Characterization

Peter J Krell 1, Donald B Stoltz 1
PMCID: PMC353272  PMID: 16789176

Abstract

A baculovirus present in the female reproductive tract of the parasitoid wasp Apanteles melanoscelus has been isolated and partially characterized. Viral DNA is double stranded, circular, and of highly variable molecular weight ranging from 2 × 106 to 25 × 106; the DNA is of homogeneous density at ρ = 1.694 g/ml. Acrylamide gels resolve 18 polypeptide bands in the case of purified virions; four to five of these appear in a semipurified nucleocapsid preparation. The electrophoretic profiles obtained are compared with those of two other baculoviruses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Arif B. M., Brown K. W. Purification and properties of a nuclear polyhedrosis virus from Choristoneura fumiferana. Can J Microbiol. 1975 Aug;21(8):1224–1231. doi: 10.1139/m75-183. [DOI] [PubMed] [Google Scholar]
  2. Bell C. D., Orlob G. B. Serological studies on virions and polyhedron protein of a nuclear polyhedrosis virus of the cabbage looper, Trichoplusia ni. Virology. 1977 May 1;78(1):162–172. doi: 10.1016/0042-6822(77)90088-5. [DOI] [PubMed] [Google Scholar]
  3. Bosslet K., Sauer G. Biological properties and physical map of the genome of a new papovavirus, HD virus. J Virol. 1978 Feb;25(2):596–607. doi: 10.1128/jvi.25.2.596-607.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Brown D. A., Bud H. M., Kelly D. C. Biophysical properties of the structural components of a granulosis virus isolated from the cabbage white butterfly (Pieris brassicae). Virology. 1977 Sep;81(2):317–327. doi: 10.1016/0042-6822(77)90148-9. [DOI] [PubMed] [Google Scholar]
  5. Burlingham B. T., Brown D. T., Doerfler W. Incomplete particles of adenovirus. I. Characteristics of the DNA associated with incomplete adenovirions of types 2 and 12. Virology. 1974 Aug;60(2):419–430. doi: 10.1016/0042-6822(74)90336-5. [DOI] [PubMed] [Google Scholar]
  6. Carroll D., O'Neill F. J. Genome maps of simian virus 40 defectives propagated in human glioblastoma cells. Virology. 1978 Jun 1;87(1):120–129. doi: 10.1016/0042-6822(78)90164-2. [DOI] [PubMed] [Google Scholar]
  7. Cohen J. C., Perdue M. L., Randall C. C., O'Callaghan D. J. Herpesvirus transcription: altered regulation induced by FUdR. Virology. 1977 Feb;76(2):621–633. doi: 10.1016/0042-6822(77)90244-6. [DOI] [PubMed] [Google Scholar]
  8. Goff S. P., Berg P. Structure and formation of circular dimers of simian virus 40 DNA. J Virol. 1977 Oct;24(1):295–302. doi: 10.1128/jvi.24.1.295-302.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harrap K. A., Payne C. C., Robertson J. S. The properties of three baculoviruses from closely related hosts. Virology. 1977 Jun 1;79(1):14–31. doi: 10.1016/0042-6822(77)90330-0. [DOI] [PubMed] [Google Scholar]
  10. Helling R. B., Goodman H. M., Boyer H. W. Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis. J Virol. 1974 Nov;14(5):1235–1244. doi: 10.1128/jvi.14.5.1235-1244.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Huang A. S. Defective interfering viruses. Annu Rev Microbiol. 1973;27:101–117. doi: 10.1146/annurev.mi.27.100173.000533. [DOI] [PubMed] [Google Scholar]
  12. Jaspars E. M. Plant viruses with a multipartite genome. Adv Virus Res. 1974;19:37–149. doi: 10.1016/s0065-3527(08)60659-4. [DOI] [PubMed] [Google Scholar]
  13. KUNITZ M. Crystalline desoxyribonuclease; isolation and general properties; spectrophotometric method for the measurement of desoxyribonuclease activity. J Gen Physiol. 1950 Mar;33(4):349–362. doi: 10.1085/jgp.33.4.349. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Lane L. C. The bromoviruses. Adv Virus Res. 1974;19:151–220. doi: 10.1016/s0065-3527(08)60660-0. [DOI] [PubMed] [Google Scholar]
  15. Lazowska J., Slonimski P. P. Electron microscopy of analysis of circular repetitive mitochondrial DNA molecules from genetically characterized rho- mutants of Saccharomyces cerevisiae. Mol Gen Genet. 1976 Jul 5;146(1):61–78. doi: 10.1007/BF00267984. [DOI] [PubMed] [Google Scholar]
  16. Lister R. M. Tobacco rattle, NETU, viruses in relation to functional heterogeneity in plant viruses. Fed Proc. 1969 Nov-Dec;28(6):1875–1889. [PubMed] [Google Scholar]
  17. Payne C. C., Compson D., de looze S. M. Properties of the nucleocapsids of a virus isolated from Oryctes rhinoceros. Virology. 1977 Mar;77(1):269–280. doi: 10.1016/0042-6822(77)90424-x. [DOI] [PubMed] [Google Scholar]
  18. Pettersson U., Mulder C., Deluis H., Sharp P. A. Cleavage of adenovirus type 2 DNA into six unique fragments by endonuclease R-RI. Proc Natl Acad Sci U S A. 1973 Jan;70(1):200–204. doi: 10.1073/pnas.70.1.200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Roberts T. M., Koths K. E. The blue-green alga agmenellum quadruplicatum contains covalently closed DNA circles. Cell. 1976 Dec;9(4 Pt 1):551–557. doi: 10.1016/0092-8674(76)90037-4. [DOI] [PubMed] [Google Scholar]
  20. SCHILDKRAUT C. L., MARMUR J., DOTY P. Determination of the base composition of deoxyribonucleic acid from its buoyant density in CsCl. J Mol Biol. 1962 Jun;4:430–443. doi: 10.1016/s0022-2836(62)80100-4. [DOI] [PubMed] [Google Scholar]
  21. Sanger F., Air G. M., Barrell B. G., Brown N. L., Coulson A. R., Fiddes C. A., Hutchison C. A., Slocombe P. M., Smith M. Nucleotide sequence of bacteriophage phi X174 DNA. Nature. 1977 Feb 24;265(5596):687–695. doi: 10.1038/265687a0. [DOI] [PubMed] [Google Scholar]
  22. Sinsheimer R. L. Recombinant DNA. Annu Rev Biochem. 1977;46:415–438. doi: 10.1146/annurev.bi.46.070177.002215. [DOI] [PubMed] [Google Scholar]
  23. Stoltz D. B., Vinson S. B., MacKinnon E. A. Baculovirus-like particles in the reproductive tracts of female parasitoid wasps. Can J Microbiol. 1976 Jul;22(7):1013–1023. doi: 10.1139/m76-148. [DOI] [PubMed] [Google Scholar]
  24. Summers M. D., Anderson D. L. Characterization of nuclear polyhedrosis virus DNAs. J Virol. 1973 Dec;12(6):1336–1346. doi: 10.1128/jvi.12.6.1336-1346.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Summers M. D. Electron microscopic observations on granulosis virus entry, uncoating and replication processes during infection of the midgut cells of Trichoplusia ni. J Ultrastruct Res. 1971 Jun;35(5):606–625. doi: 10.1016/s0022-5320(71)80014-x. [DOI] [PubMed] [Google Scholar]
  26. Summers M. D., Smith G. E. Baculovirus structural polypeptides. Virology. 1978 Feb;84(2):390–402. doi: 10.1016/0042-6822(78)90257-x. [DOI] [PubMed] [Google Scholar]
  27. Wheeler F. C., Benzinger R. H., Bujard H. Double-length, circular, single-stranded DNA from filamentous phage. J Virol. 1974 Sep;14(3):620–630. doi: 10.1128/jvi.14.3.620-630.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yagi Y., Clewell D. B. Plasmid-determined tetracycline resistance in Streptococcus faecalis: tandemly repeated resistance determinants in amplified forms of pAMalpha1 DNA. J Mol Biol. 1976 Apr 15;102(3):583–600. doi: 10.1016/0022-2836(76)90336-3. [DOI] [PubMed] [Google Scholar]

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