Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1979 Apr;30(1):1–13. doi: 10.1128/jvi.30.1.1-13.1979

Analysis of coliphage lambda mutations that affect Q gene activity: puq, byp, and nin5.

N Sternberg, L Enquist
PMCID: PMC353291  PMID: 158097

Abstract

We describe in this paper the isolation and characterization of a class of mutations, designated puq, that allow phage lambda to grow better under conditions that limit the synthesis of the phage Q gene product. These mutations were located between phage genes P and Q, a region of the lambda chromosome containing two gene N-independent mutations, nin5 and byp, that we also show to be puq mutations. Whereas the puq-3 and puq-16 mutations probably map under the nin5 deletion, the byp mutation maps between this deletion and the Q lambda-Q phi 80 crossover point. These mutations likely act by increasing the synthesis of the Q gene product. We demonstrate that the clear-plaque phenotype and reduced lysogenization frequency of byp mutants depend on increased Q gene activity. The significance of these results in understanding how transcription proceeds through the P-Q region of the lambda genome is discussed.

Full text

PDF
1

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adhya S., Gottesman M. Control of transcription termination. Annu Rev Biochem. 1978;47:967–996. doi: 10.1146/annurev.bi.47.070178.004535. [DOI] [PubMed] [Google Scholar]
  2. Appleyard R K. Segregation of New Lysogenic Types during Growth of a Doubly Lysogenic Strain Derived from Escherichia Coli K12. Genetics. 1954 Jul;39(4):440–452. doi: 10.1093/genetics/39.4.440. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Butler B., Echols H. Regulation of bacteriophage lambda development by gene N: properties of a mutation that bypasses N control of late protein synthesis. Virology. 1970 Feb;40(2):212–222. doi: 10.1016/0042-6822(70)90396-x. [DOI] [PubMed] [Google Scholar]
  4. Campbell A. The steric effect in lysogenization by bacteriophage lambda. II. Chromosomal attachment of the b2 mutant. Virology. 1965 Nov;27(3):340–345. doi: 10.1016/0042-6822(65)90113-3. [DOI] [PubMed] [Google Scholar]
  5. Dambly C., Couturier M. A minor Q-independent pathway for the expression of the late genes in bacteriophage lambda. Mol Gen Genet. 1971;113(3):244–250. doi: 10.1007/BF00339545. [DOI] [PubMed] [Google Scholar]
  6. Dambly C., Delstanche M., Gathoye A. M. qin101: Promoter mutation which allows the constitutive expression of the late genes. J Virol. 1979 Apr;30(1):14–20. doi: 10.1128/jvi.30.1.14-20.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Dennert G., Henning U. Tyrosine-incorporating amber suppressors in Escherichia coli K12. J Mol Biol. 1968 Apr 14;33(1):327–329. doi: 10.1016/0022-2836(68)90300-8. [DOI] [PubMed] [Google Scholar]
  8. Gorini L. Informational suppression. Annu Rev Genet. 1970;4:107–134. doi: 10.1146/annurev.ge.04.120170.000543. [DOI] [PubMed] [Google Scholar]
  9. Herskowitz I. Control of gene expression in bacteriophage lambda. Annu Rev Genet. 1973;7:289–324. doi: 10.1146/annurev.ge.07.120173.001445. [DOI] [PubMed] [Google Scholar]
  10. Hopkins N. Bypassing a positive regulator: isolation of a lambda mutant that does not require N product to grow. Virology. 1970 Feb;40(2):223–229. doi: 10.1016/0042-6822(70)90397-1. [DOI] [PubMed] [Google Scholar]
  11. Kourilsky P., Marcaud L., Sheldrick P., Luzzati D., Gros F. Studies of the messenger RNA of bacteriophage lambda, I. Various species synthesized early after induction of the prophage. Proc Natl Acad Sci U S A. 1968 Nov;61(3):1013–1020. doi: 10.1073/pnas.61.3.1013. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Kumar S., Bovre K., Guha A., Hradecna Z., Maher V. R., Szybalski W. Orientation and control of transcription in E. coli phage lambda. Nature. 1969 Mar 1;221(5183):823–825. doi: 10.1038/221823a0. [DOI] [PubMed] [Google Scholar]
  13. Packman S., Sly W. S. Constitutive lambda DNA replication by lambda-C17, a regulatory mutant related to virulence. Virology. 1968 Apr;34(4):778–789. doi: 10.1016/0042-6822(68)90099-8. [DOI] [PubMed] [Google Scholar]
  14. Parkinson J. S., Huskey R. J. Deletion mutants of bacteriophage lambda. I. Isolation and initial characterization. J Mol Biol. 1971 Mar 14;56(2):369–384. doi: 10.1016/0022-2836(71)90471-2. [DOI] [PubMed] [Google Scholar]
  15. Roberts J. W. Promoter mutation in vitro. Nature. 1969 Aug 2;223(5205):480–482. doi: 10.1038/223480a0. [DOI] [PubMed] [Google Scholar]
  16. Roberts J. W. Termination factor for RNA synthesis. Nature. 1969 Dec 20;224(5225):1168–1174. doi: 10.1038/2241168a0. [DOI] [PubMed] [Google Scholar]
  17. Roberts J. W. Transcription termination and late control in phage lambda. Proc Natl Acad Sci U S A. 1975 Sep;72(9):3300–3304. doi: 10.1073/pnas.72.9.3300. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Schrenk W. J., Weisberg R. A. A simple method for making new transducing lines of coliphage lambda. Mol Gen Genet. 1975;137(2):101–107. doi: 10.1007/BF00341676. [DOI] [PubMed] [Google Scholar]
  19. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  20. Shizuya H., Brown D., Campbell A. DNA polymerase I-dependent mutants of coliphage lambda. J Virol. 1974 May;13(5):947–952. doi: 10.1128/jvi.13.5.947-952.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Silva L. H., Jacob F. Etude genétique d'une mutation modifiant la sensibilité à l'immunité chez le bacteriophage lambda. Ann Inst Pasteur (Paris) 1968 Aug;115(2):145–158. [PubMed] [Google Scholar]
  22. Sternberg N. A class of rifR RNA polymerase mutations that interferes with the expression of coliphage lambda late gene. Virology. 1976 Aug;73(1):139–154. doi: 10.1016/0042-6822(76)90068-4. [DOI] [PubMed] [Google Scholar]
  23. Sternberg N. A genetic analysis of bacteriophage lambda head assembly. Virology. 1976 Jun;71(2):568–582. doi: 10.1016/0042-6822(76)90382-2. [DOI] [PubMed] [Google Scholar]
  24. Sternberg N., Tiemeier D., Enquist L. In vitro packaging of a lambda Dam vector containing EcoRI DNA fragments of Escherichia coli and phage P1. Gene. 1977 May;1(3-4):255–280. doi: 10.1016/0378-1119(77)90049-x. [DOI] [PubMed] [Google Scholar]
  25. Sternberg N., Weisberg R. Packaging of prophage and host DNA by coliphage lambda. Nature. 1975 Jul 10;256(5513):97–103. doi: 10.1038/256097a0. [DOI] [PubMed] [Google Scholar]
  26. Szpirer J., Brachet P. Relations physiologiques entre les phages tempérés lambda et phi80. Mol Gen Genet. 1970;108(1):78–92. doi: 10.1007/BF00343187. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES