Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1979 Apr;30(1):38–44. doi: 10.1128/jvi.30.1.38-44.1979

Generation of defective interfering particles of Semliki Forest virus in a clone of Aedes albopictus (mosquito) cells.

K B Logan
PMCID: PMC353295  PMID: 480458

Abstract

Serial undiluted passage of Semliki Forest virus in a clone of Aedes albopictus cells resulted in a marked decrease in infectious virus yields due to the generation and accumulation of defective interfering particles. Virus from the third passage had a high particle/infectivity ratio and interfered specifically with homologous but not heterologous standard virus replication. Two RNA species of molecular weights 0.78 X 10(6) and 0.61 X 10(6) were the major RNA components of purified passage 4 virus. These RNA species were also the predominant virus RNA species detected in cells infected with passage 3 virus. Synthesis of standard virus RNA and virus-specified protein was much reduced in passage 3 virus-infected cells. Interference with standard virus replication and the synthesis of large amounts of defective interfering RNA were also observed in chicken embryo cells infected with passage 3 virus from mosquito cells.

Full text

PDF
38

Images in this article

42Image
on p.42

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Atkins G. J., Samuels J., Kennedy S. I. Isolation and preliminary characterization of temperature-sensitive mutants of Sindbis virus strain AR339. J Gen Virol. 1974 Dec;25(3):371–380. doi: 10.1099/0022-1317-25-3-371. [DOI] [PubMed] [Google Scholar]
  2. Bruton C. J., Porter A., Kennedy S. I. Defective-interfering particles of Semliki Forest virus: intracellular events during interference. J Gen Virol. 1976 Jun;31(3):397–416. doi: 10.1099/0022-1317-31-3-397. [DOI] [PubMed] [Google Scholar]
  3. Cancedda R., Schlesinger M. J. Formation of Sindbis virus capsid protein in mammalian cell-free extracts programmed with viral messenger RNA. Proc Natl Acad Sci U S A. 1974 May;71(5):1843–1847. doi: 10.1073/pnas.71.5.1843. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Clegg J. C., Brzeski H., Kennedy S. I. RNA polymerase components in Semliki Forest virus-infected cells: synthesis from large precursors. J Gen Virol. 1976 Sep;32(3):413–430. doi: 10.1099/0022-1317-32-3-413. [DOI] [PubMed] [Google Scholar]
  5. Clegg J. C., Kennedy S. I. In vitro synthesis of structural proteins of Semliki Forest virus directed by isolated 26 S RNA from infected cells. FEBS Lett. 1974 Jun 15;42(3):327–330. doi: 10.1016/0014-5793(74)80757-x. [DOI] [PubMed] [Google Scholar]
  6. Clewley J. P., Kennedy S. I. Purification and polypeptide composition of Semliki Forest virus RNA polymerase. J Gen Virol. 1976 Sep;32(3):395–411. doi: 10.1099/0022-1317-32-3-395. [DOI] [PubMed] [Google Scholar]
  7. Davey M. W., Dennett D. P., Dalgarno L. The growth of two togaviruses in cultured mosquito and vertebrate cells. J Gen Virol. 1973 Aug;20(2):225–232. doi: 10.1099/0022-1317-20-2-225. [DOI] [PubMed] [Google Scholar]
  8. EAGLE H. Amino acid metabolism in mammalian cell cultures. Science. 1959 Aug 21;130(3373):432–437. doi: 10.1126/science.130.3373.432. [DOI] [PubMed] [Google Scholar]
  9. Eaton B. T. Defective interfering particles of Semliki Forest virus generated in BHK cells do not interfere with viral RNA synthesis in Aedes albopictus cells. Virology. 1975 Dec;68(2):534–538. doi: 10.1016/0042-6822(75)90293-7. [DOI] [PubMed] [Google Scholar]
  10. Eaton B. T. Evidence for the synthesis of defection interfering particles by Aedes albopictus cells persistently infected with Sindbis virus. Virology. 1977 Apr;77(2):843–848. doi: 10.1016/0042-6822(77)90503-7. [DOI] [PubMed] [Google Scholar]
  11. Eaton B. T., Faulkner P. Altered pattern of viral RNA synthesis in cells infected with standard and defective Sindbis virus. Virology. 1973 Jan;51(1):85–93. doi: 10.1016/0042-6822(73)90368-1. [DOI] [PubMed] [Google Scholar]
  12. Grimley P. M., Berezesky I. K., Friedman R. M. Cytoplasmic structures associated with an arbovirus infection: loci of viral ribonucleic acid synthesis. J Virol. 1968 Nov;2(11):1326–1338. doi: 10.1128/jvi.2.11.1326-1338.1968. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Guild G. M., Stollar V. Defective interfering particles of Sindbis virus. III. Intracellular viral RNA species in chick embryo cell cultures. Virology. 1975 Sep;67(1):24–41. doi: 10.1016/0042-6822(75)90400-6. [DOI] [PubMed] [Google Scholar]
  14. Guild G. M., Stollar V. Defective interfering particles of Sindbis virus. V. Sequence relationships between SVSTD 42 S RNA and intracellular defective viral RNAs. Virology. 1977 Mar;77(1):175–188. doi: 10.1016/0042-6822(77)90416-0. [DOI] [PubMed] [Google Scholar]
  15. Igarashi A. Isolation of a Singh's Aedes albopictus cell clone sensitive to Dengue and Chikungunya viruses. J Gen Virol. 1978 Sep;40(3):531–544. doi: 10.1099/0022-1317-40-3-531. [DOI] [PubMed] [Google Scholar]
  16. Igarashi A., Koo R., Stollar V. Evolution and properties of Aedes albopictus cell cultures persistently infected with sindbis virus. Virology. 1977 Oct 1;82(1):69–83. doi: 10.1016/0042-6822(77)90033-2. [DOI] [PubMed] [Google Scholar]
  17. Igarashi A., Stollar V. Failure of defective interfering particles of Sindbis virus produced in BHK or chicken cells to affect viral replication in Aedes albopictus cells. J Virol. 1976 Aug;19(2):398–408. doi: 10.1128/jvi.19.2.398-408.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kang C. Y., Allen R. Host function-dependent induction of defective interfering particles of vesicular stomatitis virus. J Virol. 1978 Jan;25(1):202–206. doi: 10.1128/jvi.25.1.202-206.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Kennedy S. I. Sequence relationships between the genome and the intracellular RNA species of standard and defective-interfering Semliki Forest virus. J Mol Biol. 1976 Dec;108(2):491–511. doi: 10.1016/s0022-2836(76)80132-5. [DOI] [PubMed] [Google Scholar]
  20. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  21. Morser M. J., Kennedy S. I., Burke D. C. Virus-specified polypeptides in cells infected with Semliki Forest virus. J Gen Virol. 1973 Oct;21:19–29. doi: 10.1099/0022-1317-21-1-19. [DOI] [PubMed] [Google Scholar]
  22. Sarver N., Stollar V. Sindbis virus-induced cytopathic effect in clones of Aedes albopictus (Singh) cells. Virology. 1977 Jul 15;80(2):390–400. doi: 10.1016/s0042-6822(77)80014-7. [DOI] [PubMed] [Google Scholar]
  23. Schlesinger S., Schlesinger M., Burge B. W. Defective virus particles from Sindbis virus. Virology. 1972 May;48(2):615–617. doi: 10.1016/0042-6822(72)90076-1. [DOI] [PubMed] [Google Scholar]
  24. Shenk T. E., Stollar V. Defective-interfering particles of Sindbis virus. I. Isolation and some chemical and biological properties. Virology. 1973 May;53(1):162–173. doi: 10.1016/0042-6822(73)90475-3. [DOI] [PubMed] [Google Scholar]
  25. Shenk T. E., Stollar V. Defective-interfering particles of Sindbis virus. II. Homologous interference. Virology. 1973 Oct;55(2):530–534. doi: 10.1016/0042-6822(73)90197-9. [DOI] [PubMed] [Google Scholar]
  26. Simmons D. T., Strauss J. H. Translation of Sindbis virus 26 S RNA and 49 S RNA in lysates of rabbit reticulocytes. J Mol Biol. 1974 Jun 25;86(2):397–409. doi: 10.1016/0022-2836(74)90027-8. [DOI] [PubMed] [Google Scholar]
  27. Stark C., Kennedy S. I. The generation and propagation of defective-interfering particles of Semliki Forest virus in different cell types. Virology. 1978 Aug;89(1):285–299. doi: 10.1016/0042-6822(78)90060-0. [DOI] [PubMed] [Google Scholar]
  28. Stevens T. M. Arbovirus replication in mosquito cell lines (Singh) grown in monolayer or suspension culture. Proc Soc Exp Biol Med. 1970 May;134(1):356–361. doi: 10.3181/00379727-134-34793. [DOI] [PubMed] [Google Scholar]
  29. Stollar V., Shenk T. E. Homologous viral interference in Aedes albopictus cultures chronically infected with Sindbis virus. J Virol. 1973 Apr;11(4):592–595. doi: 10.1128/jvi.11.4.592-595.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stollar V., Shenk T. E., Koo R., Igarashi A., Schlesinger R. W. Observations of Aedes albopictus cell cultures persistently infected with Sindbis virus. Ann N Y Acad Sci. 1975;266:214–231. doi: 10.1111/j.1749-6632.1975.tb35103.x. [DOI] [PubMed] [Google Scholar]
  31. Weiss B., Schlesinger S. Defective interfering passages of Sindbis virus: chemical composition, biological activity, and mode of interference. J Virol. 1973 Oct;12(4):862–871. doi: 10.1128/jvi.12.4.862-871.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Wengler G., Beato M., Hackemack B. A. Translation of 26 S virus-specific RNA from Semliki Forest virus-infected cells in vitro. Virology. 1974 Sep;61(1):120–128. doi: 10.1016/0042-6822(74)90247-5. [DOI] [PubMed] [Google Scholar]
  33. Wengler G., Wengler G. Localization of the 26-S RNA sequence on the viral genome type 42-S RNA isolated from SFV-infected cells. Virology. 1976 Aug;73(1):190–199. doi: 10.1016/0042-6822(76)90073-8. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES