Abstract
Viral DNA was isolated from human and murine cytomegalovirus by equilibrium centrifugation in cesium chloride gradients. The size of the DNA was measured relative to T4 DNA by velocity sedimentation in neutral glycerol gradients, and fractions were assayed for infectious DNA. Infectious murine cytomegalovirus DNA sedimented as a single peak with an estimated molecular weight of 136 X 10(6). Infectious human cytomegalovirus DNA was detected in two peaks with molecular weights of 130 X 10(6) and 150 X 10(6).
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Freifelder D. Molecular weights of coliphages and coliphage DNA. IV. Molecular weights of DNA from bacteriophages T4, T5 and T7 and the general problem of determination of M. J Mol Biol. 1970 Dec 28;54(3):567–577. doi: 10.1016/0022-2836(70)90127-0. [DOI] [PubMed] [Google Scholar]
- Geelen J. L., Walig C., Wertheim P., van der Noordaa J. Human cytomegalovirus DNA. I. Molecular weight and infectivity. J Virol. 1978 Jun;26(3):813–816. doi: 10.1128/jvi.26.3.813-816.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Graham F. L., Veldhuisen G., Wilkie N. M. Infectious herpesvirus DNA. Nat New Biol. 1973 Oct 31;245(148):265–266. doi: 10.1038/newbio245265a0. [DOI] [PubMed] [Google Scholar]
- Huang E. S., Chen S. T., Pagano J. S. Human cytomegalovirus. I. Purification and characterization of viral DNA. J Virol. 1973 Dec;12(6):1473–1481. doi: 10.1128/jvi.12.6.1473-1481.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Huang E. S., Kilpatrick B. A., Huang Y. T., Pagano J. S. Detection of human cytomegalovirus and analysis of strain variation. Yale J Biol Med. 1976 Mar;49(1):29–43. [PMC free article] [PubMed] [Google Scholar]
- Huang E. S., Pagano J. S. Human cytomegalovirus. II. Lack of relatedness to DNA of herpes simples I and II, Epstein-Barr virus, and nonhuman strains of cytomegalovirus. J Virol. 1974 Mar;13(3):642–645. doi: 10.1128/jvi.13.3.642-645.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jean J. H., Yoshimura N., Furukawa T., Plotkin S. A. Intracellular forms of the parental human cytomegalovirus genome at early stages of the infective process. Virology. 1978 May 1;86(1):281–286. doi: 10.1016/0042-6822(78)90029-6. [DOI] [PubMed] [Google Scholar]
- Kieff E. D., Bachenheimer S. L., Roizman B. Size, composition, and structure of the deoxyribonucleic acid of herpes simplex virus subtypes 1 and 2. J Virol. 1971 Aug;8(2):125–132. doi: 10.1128/jvi.8.2.125-132.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kilpatrick B. A., Huang E. S. Human cytomegalovirus genome: partial denaturation map and organization of genome sequences. J Virol. 1977 Oct;24(1):261–276. doi: 10.1128/jvi.24.1.261-276.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lindahl T., Adams A., Bjursell G., Bornkamm G. W., Kaschka-Dierich C., Jehn U. Covalently closed circular duplex DNA of Epstein-Barr virus in a human lymphoid cell line. J Mol Biol. 1976 Apr 15;102(3):511–530. doi: 10.1016/0022-2836(76)90331-4. [DOI] [PubMed] [Google Scholar]
- Mosmann T. R., Hudson J. B. Some properties of the genome of murine cytomegalovirus (MCV). Virology. 1973 Jul;54(1):135–149. doi: 10.1016/0042-6822(73)90123-2. [DOI] [PubMed] [Google Scholar]
- Mosmann T. R., Hudson J. B. Structural and functional heterogeneity of the murine cytomegalovirus genome. Virology. 1974 Nov;62(1):175–183. doi: 10.1016/0042-6822(74)90313-4. [DOI] [PubMed] [Google Scholar]
- Sarov I., Friedman A. Electron microscopy of human cytomegalovirus DNA. Arch Virol. 1976;50(4):343–347. doi: 10.1007/BF01317960. [DOI] [PubMed] [Google Scholar]
- Stow N. D., Wilkie N. M. An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. J Gen Virol. 1976 Dec;33(3):447–458. doi: 10.1099/0022-1317-33-3-447. [DOI] [PubMed] [Google Scholar]