Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1979 May;30(2):543–550. doi: 10.1128/jvi.30.2.543-550.1979

Transduction of bacteriophage lambda by bacteriophage T1.

H Drexler, J R Christensen
PMCID: PMC353358  PMID: 381686

Abstract

When bacteriophage T1 was grown on bacteriophage lambda-lysogenic cells, phenotypically mixed particles were formed which had the serum sensitivity, host range, and density of T1 but which gave rise to lambda phage. T1 packaged lambda genomes more efficiently both when the length of the prophage was less than that of wild-type lambda and when the host cell was polylysogenic. Expression of the red genes of lambda or the recE system of Escherichia coli during T1 growth enhanced pickup of lambda by T1, whereas packaging was reduced in recB cells. If donors were singly lysogenic, the expression of transduced lambda genomes as a PFU required lambda-specified excisive recombination, whereas lambda genomes transduced from polylysogens required only lambda- or E. coli-specified general recombination to give a productive infection.

Full text

PDF
543

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bachmann B. J., Low K. B., Taylor A. L. Recalibrated linkage map of Escherichia coli K-12. Bacteriol Rev. 1976 Mar;40(1):116–167. doi: 10.1128/br.40.1.116-167.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Barbour S. D., Nagaishi H., Templin A., Clark A. J. Biochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations. Proc Natl Acad Sci U S A. 1970 Sep;67(1):128–135. doi: 10.1073/pnas.67.1.128. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bendig M. M., Drexler H. Transduction of bacteriophage Mu by bacteriophage T1. J Virol. 1977 Jun;22(3):640–645. doi: 10.1128/jvi.22.3.640-645.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. CAMPBELL A. Sensitive mutants of bacteriophage lambda. Virology. 1961 May;14:22–32. doi: 10.1016/0042-6822(61)90128-3. [DOI] [PubMed] [Google Scholar]
  5. Clark A. J. Progress toward a metabolic interpretation of genetic recombination of Escherichia coli and bacteriophage lambda. Genetics. 1974 Sep;78(1):259–271. doi: 10.1093/genetics/78.1.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Davis R. W., Parkinson J. S. Deletion mutants of bacteriophage lambda. 3. Physical structure of att-phi. J Mol Biol. 1971 Mar 14;56(2):403–423. doi: 10.1016/0022-2836(71)90473-6. [DOI] [PubMed] [Google Scholar]
  7. Drexler H. Specialized transduction of the biotin region of Escherichia coli by phage T1. Mol Gen Genet. 1977 Mar 28;152(1):59–63. doi: 10.1007/BF00264940. [DOI] [PubMed] [Google Scholar]
  8. Drexler H. Transduction by bacteriophage T1. Proc Natl Acad Sci U S A. 1970 Aug;66(4):1083–1088. doi: 10.1073/pnas.66.4.1083. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Eisenstark A. Genetic recombination in bacteria. Annu Rev Genet. 1977;11:369–396. doi: 10.1146/annurev.ge.11.120177.002101. [DOI] [PubMed] [Google Scholar]
  10. Figurski D. H., Christensen J. R. Functional characterization of the genes of bacteriophage T1. Virology. 1974 Jun;59(2):397–407. doi: 10.1016/0042-6822(74)90453-x. [DOI] [PubMed] [Google Scholar]
  11. Geiman J. M., Christensen J. R., Drexler H. Interactions between the vegetative states of phages lambda and T1. J Virol. 1974 Dec;14(6):1430–1434. doi: 10.1128/jvi.14.6.1430-1434.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gill G. S., MacHattie L. A. Limited permutations of the nucleotide sequence in bacteriophage T1 DNA. J Mol Biol. 1976 Jun 25;104(2):505–515. doi: 10.1016/0022-2836(76)90284-9. [DOI] [PubMed] [Google Scholar]
  13. Gillen J. R., Karu A. E., Nagaishi H., Clark A. J. Characterization of the deoxyribonuclease determined by lambda reverse as exonuclease VIII of Escherichia coli. J Mol Biol. 1977 Jun 15;113(1):27–41. doi: 10.1016/0022-2836(77)90039-0. [DOI] [PubMed] [Google Scholar]
  14. Gottesman M. E., Yarmolinsky M. B. Integration-negative mutants of bacteriophage lambda. J Mol Biol. 1968 Feb 14;31(3):487–505. doi: 10.1016/0022-2836(68)90423-3. [DOI] [PubMed] [Google Scholar]
  15. Guarneros G., Echols H. New mutants of bacteriophage lambda with a specific defect in excision from the host chromosome. J Mol Biol. 1970 Feb 14;47(3):565–574. doi: 10.1016/0022-2836(70)90323-2. [DOI] [PubMed] [Google Scholar]
  16. JACOB F., WOLLMAN E. L. Sur les processus de conjugaison et de recombinaison chez Escherichia coli. I. L'induction par conjugaison ou induction zygotique. Ann Inst Pasteur (Paris) 1956 Oct;91(4):486–510. [PubMed] [Google Scholar]
  17. Kushner S. R., Nagaishi H., Templin A., Clark A. J. Genetic recombination in Escherichia coli: the role of exonuclease I. Proc Natl Acad Sci U S A. 1971 Apr;68(4):824–827. doi: 10.1073/pnas.68.4.824. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Kylberg K. J., Bendig M. M., Drexler H. Characterization of transduction by bacteriophage T1: time of production and density of transducing particles. J Virol. 1975 Oct;16(4):854–858. doi: 10.1128/jvi.16.4.854-858.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Michalke W. Erhöhte Rekombinationshäufigkeit an den Enden des T1-Chromosoms. Mol Gen Genet. 1967;99(1):12–33. doi: 10.1007/BF00306454. [DOI] [PubMed] [Google Scholar]
  20. Parkinson J. S. Deletion mutants of bacteriophage lambda. II. Genetic properties of att-defective mutants. J Mol Biol. 1971 Mar 14;56(2):385–401. doi: 10.1016/0022-2836(71)90472-4. [DOI] [PubMed] [Google Scholar]
  21. SUSSMAN R., JACOB F. [On a thermosensitive repression system in the Escherichia coli lambda bacteriophage]. C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517–1519. [PubMed] [Google Scholar]
  22. Shimada K., Weisberg R. A., Gottesman M. E. Prophage lambda at unusual chromosomal locations. I. Location of the secondary attachment sites and the properties of the lysogens. J Mol Biol. 1972 Feb 14;63(3):483–503. doi: 10.1016/0022-2836(72)90443-3. [DOI] [PubMed] [Google Scholar]
  23. Signer E. R., Weil J. Recombination in bacteriophage lambda. I. Mutants deficient in general recombination. J Mol Biol. 1968 Jul 14;34(2):261–271. doi: 10.1016/0022-2836(68)90251-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES