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. 2012 Dec 13;138(1):34–46. doi: 10.1111/imm.12010

Figure 1.

Figure 1

Processing and presentation of Mycobacterium tuberculosis (Mtb) antigens by monocyte-derived macrophages (MDMs) pre-treated with interferon-γ (IFN-γ) or interleukin-10 (IL-10). The MDMs were pre-treated with or without 50 U/ml IFN-γ or with 5 ng/ml IL-10 for 24 hr, washed and pulsed with heat-killed (a and c) or live (b and d) Mtb for 60 min at a multiplicity of infection of 30 and fixed after a 30, 60 or 120min incubation at 37°. Polyclonal autologous Mtb-specific CD4+ T cells were used to detect Mtb peptide–MHC-II complexes, and the supernatants were assessed for IFN-γ by ELISA. (a and b) Processing by IFN-γ-treated MDMs; (c and d) processing by IL-10-treated MDMs. Data are representative of four independent experiments.