Table 2.
Influence of reaction conditions on C=C isomerization and reduction of substrate 1 a
| Substrate |  |
|||
|---|---|---|---|---|
| Products |  |
 |
||
| Protein | Cofactor | Conv. [%] | Conv. [%] | |
| 1 | OYE2 | none | <1 | <1 |
| 2 | none | NADH | <1 | <1 |
| 3 | none | NAD+ | <1 | <1 |
| 4 | none | NAD+/NADH (1:1) | <1 | <1 |
| 5 | OYE2 | NAD+ | <1 | <1 |
| 6 | OYE2 | NADH | 74 | 26 |
| 7 | OYE2 | NAD+/NADH (1:1) | 78 | 21 |
| 8 | OYE2 | NADPH | 30 | 2 |
| 9 | albumin | none | <1 | <1 |
| 10 | albumin | NADH | <1 | <1 |
| 11 | albumin | NAD+ | <1 | <1 |
Reaction conditions: substrate (10 mm), protein (OYE2, concentration 100 μg mL−1), albumin (≍1 mg), cofactor (15 mm), Tris⋅HCl buffer (50 mm, pH 7.5), 24 h, 30 °C, 120 rpm.