Table 3.
Substrate | Reduction | Isomerization | ||||
---|---|---|---|---|---|---|
KM [mm] | kcat [s−1] | kcat/KM [s−1 mm−1] | KM [mm] | kcat [s−1] | kcat/KM [s−1 mm−1] | |
0.76±0.09 | 0.015±0.0005 | 1.97×10−8 | 1.62±0.28 | 0.10±0.01 | 6.18×10−8 | |
1.12±0.06 | 0.0056±0.0003 | 0.50×10−8 | n.r. | n.r. | n.r. |
Reaction conditions: substrate (0.1–4 mm), Tris⋅HCl buffer (50 mm, pH 7.5), NADH (15 mm), 2 h, 30 °C. n.r. no reaction.