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. 2012 Sep 28;13(16):2346–2351. doi: 10.1002/cbic.201200475

Table 3.

Kinetic constants for C=C bond reduction and isomerization of regioisomeric substrates 1 a and 2 a in the presence of OYE2 (average values of triplicate measurements)[a]

Substrate Reduction Isomerization
KM [mm] kcat [s−1] kcat/KM [s−1 mm−1] KM [mm] kcat [s−1] kcat/KM [s−1 mm−1]
Inline graphic 0.76±0.09 0.015±0.0005 1.97×10−8 1.62±0.28 0.10±0.01 6.18×10−8
Inline graphic 1.12±0.06 0.0056±0.0003 0.50×10−8 n.r. n.r. n.r.
[a]

Reaction conditions: substrate (0.1–4 mm), TrisHCl buffer (50 mm, pH 7.5), NADH (15 mm), 2 h, 30 °C. n.r. no reaction.