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. 1979 Jun;30(3):946–950. doi: 10.1128/jvi.30.3.946-950.1979

Nucleotide sequence of the self-priming 3' terminus of the single-stranded DNA extracted from the parvovirus Kilham rat virus.

L A Salzman, P Fabisch
PMCID: PMC353409  PMID: 225552

Abstract

The parvovirus genome is a linear, single-stranded DNA molecule with double-stranded hairpin termini. The 3' terminus can serve in vitro as a self-primer for the synthesis of a double-stranded viral DNA intermediate. We have sequenced the nucleotides in the 3' terminus and propose a model for the secondary structure of the terminus and the in vitro origin of replication for the complementary viral DNA strand.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bastia D. The nucleotide sequence surrounding the origin of DNA replication of Col E1. Nucleic Acids Res. 1977 Sep;4(9):3123–3142. doi: 10.1093/nar/4.9.3123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bourguignon G. J., Tattersall P. J., Ward D. C. DNA of minute virus of mice: self-priming, nonpermuted, single-stranded genome with a 5'-terminal hairpin duplex. J Virol. 1976 Oct;20(1):290–306. doi: 10.1128/jvi.20.1.290-306.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Englund P. T. The 3'-terminal nucleotide sequences of T7 DNA. J Mol Biol. 1972 May 14;66(2):209–224. doi: 10.1016/0022-2836(72)90474-3. [DOI] [PubMed] [Google Scholar]
  4. Fife K. H., Berns K. I., Murray K. Structure and nucleotide sequence of the terminal regions of adeno-associated virus DNA. Virology. 1977 May 15;78(2):475–477. doi: 10.1016/0042-6822(77)90124-6. [DOI] [PubMed] [Google Scholar]
  5. Gray C. P., Sommer R., Polke C., Beck E., Schaller H. Structure of the orgin of DNA replication of bacteriophage fd. Proc Natl Acad Sci U S A. 1978 Jan;75(1):50–53. doi: 10.1073/pnas.75.1.50. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gunther M., May P. Isolation and structural characterization of monomeric and dimeric forms of replicative intermediates of Kilham rat virus DNA. J Virol. 1976 Oct;20(1):86–95. doi: 10.1128/jvi.20.1.86-95.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hinton D. M., Baez J. A., Gumport R. I. T4 RNA Ligase joins 2'-deoxyribonucleoside 3',5'-bisphosphates to oligodeoxyribonucleotides. Biochemistry. 1978 Nov 28;17(24):5091–5097. doi: 10.1021/bi00617a004. [DOI] [PubMed] [Google Scholar]
  8. Lavelle G., Li A. T. Isolation of intracellular replicative forms and progeny single strands of DNA from parvovirus KRV in sucrose gradients containing guanidine hydrochloride. Virology. 1977 Jan;76(1):464–467. doi: 10.1016/0042-6822(77)90324-5. [DOI] [PubMed] [Google Scholar]
  9. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nossal N. G. DNA synthesis on a double-stranded DNA template by the T4 bacteriophage DNA polymerase and the T4 gene 32 DNA unwinding protein. J Biol Chem. 1974 Sep 10;249(17):5668–5676. [PubMed] [Google Scholar]
  11. Reddy V. B., Thimmappaya B., Dhar R., Subramanian K. N., Zain B. S., Pan J., Ghosh P. K., Celma M. L., Weissman S. M. The genome of simian virus 40. Science. 1978 May 5;200(4341):494–502. doi: 10.1126/science.205947. [DOI] [PubMed] [Google Scholar]
  12. Rhode S. L., 3rd Replication process of the parvovirus H-1. II. Isolation and characterization of H-1 replicative form DNA. J Virol. 1974 Feb;13(2):400–410. doi: 10.1128/jvi.13.2.400-410.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Rhode S. L., 3rd Replication process of the parvovirus H-1. VI. Characterization of a replication terminus of H-1 replicative-form DNA. J Virol. 1977 Feb;21(2):694–712. doi: 10.1128/jvi.21.2.694-712.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Salzman L. A., Fabisch P., Parr R., Garon C., Wali T. In vitro synthesis of double-stranded DNA from the Kilham rat virus single-stranded DNA genome. J Virol. 1978 Sep;27(3):784–790. doi: 10.1128/jvi.27.3.784-790.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Salzman L. A., Jori L. A. Characterization of the Kilham rat virus. J Virol. 1970 Feb;5(2):114–122. doi: 10.1128/jvi.5.2.114-122.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Salzman L. A., White W. In vivo conversion of the single-stranded DNA of the kilham rat virus to a double-stranded form. J Virol. 1973 Feb;11(2):299–305. doi: 10.1128/jvi.11.2.299-305.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

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