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. 1979 Jul;31(1):253–256. doi: 10.1128/jvi.31.1.253-256.1979

Carbohydrates of influenza virus. III. Nature of oligosaccharide-protein linkage in viral glycoproteins.

W Keil, H D Klenk, R T Schwarz
PMCID: PMC353441  PMID: 501797

Abstract

Four different glycopeptides can be distinguished after pronase digestion of influenza A virus glycoproteins: Ia and Ib, containing N-acetylglucosamine, mannose, galactose, and fucose, and IIa and IIb, containing mannose and N-acetylglucosamine. All glycopeptides yielded N-acetylglucosaminyl-asparagine after mild acid hydrolysis. There was no evidence for O-glycosidic bonds. Thus, the carbohydrate complement is linked to the polypeptide exclusively by N-glycosidic linkages between N-acetylglucosamine and asparagine.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen R. The sequence determination of a protein in a micro scale: the sequence analysis of ribosomal protein L34 of Escherichia coli. Hoppe Seylers Z Physiol Chem. 1976 Jun;357(6):873–886. doi: 10.1515/bchm2.1976.357.1.873. [DOI] [PubMed] [Google Scholar]
  2. Chéron A., Fournet B., Spik G., Montreuil J. Structure complète de glycopeptides isolés des immunoglobulines IgG1 du colostrum de vache. Biochimie. 1976;58(8):927–942. doi: 10.1016/s0300-9084(76)80281-7. [DOI] [PubMed] [Google Scholar]
  3. Collins J. K., Knight C. A. Purification of the influenza hemagglutinin glycoprotein and characterization of its carbohydrate components. J Virol. 1978 May;26(2):457–467. doi: 10.1128/jvi.26.2.457-467.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Hughes R. C. The complex carbohydrates of mammalian cell surfaces and their biological roles. Essays Biochem. 1975;11:1–36. [PubMed] [Google Scholar]
  5. KNOX K. W., BRANDSEN J. The isolation of components from the cell wall of Lactobacillus casei. Biochem J. 1962 Oct;85:15–23. doi: 10.1042/bj0850015. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Montreuil J., Monsigny M., Buchet M. T. Etudes sur les glycoportéides. Action des alcalis sur les O-séryl et O-thréonyl-beta-N-acétyl-d-glucosaminides. Mécanisme de la "beta-élimination". C R Acad Sci Hebd Seances Acad Sci D. 1967 Apr 17;264(16):2068–2071. [PubMed] [Google Scholar]
  7. Moyer S. A., Tsang J. M., Atkinson P. H., Summers D. F. Oligosaccharide moieties of the glycoprotein of vesicular stomatitis virus. J Virol. 1976 Apr;18(1):167–175. doi: 10.1128/jvi.18.1.167-175.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Nakamura K., Compans R. W. Glycopeptide components of influenza viral glycoproteins. Virology. 1978 May 15;86(2):432–442. doi: 10.1016/0042-6822(78)90083-1. [DOI] [PubMed] [Google Scholar]
  9. Schwarz R. T., Fournet B., Montreuil J., Rott R., Klenk H. D. The carbohydrates of influenza virus. II. Gas chromatographic analysis of glycopeptides derived from viral glycoproteins and mucopolysaccharides. Arch Virol. 1978;56(3):251–255. doi: 10.1007/BF01317854. [DOI] [PubMed] [Google Scholar]
  10. Schwarz R. T., Schmidt M. F., Anwer U., Klenk H. D. Carbohydrates of influenza virus. I. Glycopeptides derived from viral glycoproteins after labeling with radioactive sugars. J Virol. 1977 Aug;23(2):217–226. doi: 10.1128/jvi.23.2.217-226.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Seiler N., Wiechmann J. Zum Nachweis von Aminosäuren im 10-10-Mol-Massetab. Trennung von 1-Dimethylamino-naphthalin-5-sulfonyl-aminosäuren auf Dünnschichtchromatogrammen. Experientia. 1964 Oct 15;20(10):559–560. doi: 10.1007/BF02150289. [DOI] [PubMed] [Google Scholar]

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