Abstract
We analyzed the method of exhaustive hybridization of single-stranded DNA and derived a general relationship between the fraction of the probe DNA hybridized and the sizes and copy numbers of the segments of the viral genome integrated in cellular DNA. The equations employed can be used to analyze integrated DNA comprised of overlapping and nonoverlapping segments of the viral genome. Using these equations, we analyzd the adenovirus type 2 DNA content of a series of hamster cell lines transformed by adenovirus type 2 and several adenovirus type 2-simian virus 40 hybrid viruses. We found no eividence that the integrated viral DNA is comprised of overlapsping segments. However, the number of copies of the integrated segments varies between lines cloned from the same transformed isolate, and copy numbers change during in vivo passage of transformed cells.
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Selected References
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- Breeden J. H., Wewerka-Lutz Y., Schnipper L. E., Hauser J., Patch C. T., Lewis A. M., Jr, Levine A. S. Content and expression of integrated viral DNA in hamster cells transformed by nondefective adenovirus type 2- simian virus 40 hybrid viruses. J Virol. 1976 Dec;20(3):555–563. doi: 10.1128/jvi.20.3.555-563.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johansson K., Persson H., Lewis A. M., Pettersson U., Tibbetts C., Philipson L. Viral DNA sequences and gene products in hamster cells transformed by adenovirus type 2. J Virol. 1978 Sep;27(3):628–639. doi: 10.1128/jvi.27.3.628-639.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Johansson K., Pettersson U., Philipson L., Tibbetts C. Reassociation of complementary strand-specific adenovirus type 2 DNA with viral DNA sequences of transformed cells. J Virol. 1977 Jul;23(1):29–35. doi: 10.1128/jvi.23.1.29-35.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kelly T. J., Jr, Lewis A. M., Jr Use of nondefective adenovirus-simian virus 40 hybrids for mapping the simian virus 40 genome. J Virol. 1973 Sep;12(3):643–652. doi: 10.1128/jvi.12.3.643-652.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Khoury G., Howley P., Brown M., Martin M. The detection and quantitation of SV40 nucleic acid sequences using single-stranded SV40 DNA probes. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):147–152. doi: 10.1101/sqb.1974.039.01.020. [DOI] [PubMed] [Google Scholar]
- Lewis A. M., Jr, Breeden J. H., Wewerka Y. L., Schnipper L. E., Levine A. S. Studies of hamster cells transformed by adenovirus 2 and the nondefective Ad2-SV40 hybrids. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):651–656. doi: 10.1101/sqb.1974.039.01.078. [DOI] [PubMed] [Google Scholar]
- Lewis A. M., Jr, Rabson A. S., Levine A. S. Studies of nondefective adenovirus 2-simian virus 40 hybrid viruses. Transformation of hamster kidney cells by adenovirus 2 and the nondefective hybrid viruses. J Virol. 1974 Jun;13(6):1291–1301. doi: 10.1128/jvi.13.6.1291-1301.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Morrow J. F., Berg P., Kelly T. J., Jr, Lewis A. M., Jr Mapping of simian virus 40 early functions on the viral chromosome. J Virol. 1973 Sep;12(3):653–658. doi: 10.1128/jvi.12.3.653-658.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sharp P. A., Pettersson U., Sambrook J. Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. J Mol Biol. 1974 Jul 15;86(4):709–726. doi: 10.1016/0022-2836(74)90348-9. [DOI] [PubMed] [Google Scholar]