Abstract
The RNA of infectious bursal disease virus was reexamined in a detailed analysis. It could be established that its genome consists of two segments of double-stranded RNA. The RNA is RNase resistant and has a sedimentation coefficient of 14S and a buoyant density of 1.62 g/ml. The purine/pyrimidine ratio is nearly 1; the guanine plus cytosine content is 55.3%; the Tm is 95.5 degrees C. The molecular weights of the two double-stranded segments were determined to be 2.2 x 10(6) and 2.5 x 10(6).
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- Boedtker H. Dependence of the sedimentation coefficient on molecular weight of RNA after reaction with formaldehyde. J Mol Biol. 1968 Jul 14;35(1):61–70. doi: 10.1016/s0022-2836(68)80036-1. [DOI] [PubMed] [Google Scholar]
- Boedtker H. The reaction of ribonucleic acid with formaldehyde. I. Optical absorbance studies. Biochemistry. 1967 Sep;6(9):2718–2727. doi: 10.1021/bi00861a011. [DOI] [PubMed] [Google Scholar]
- Cohen J., Poinsard A., Scherrer R. Physico-chemical and morphological features of infectious pancreatic necrosis virus. J Gen Virol. 1973 Dec;21(3):485–498. doi: 10.1099/0022-1317-21-3-485. [DOI] [PubMed] [Google Scholar]
- Dobos P. Size and structure of the genome of infectious pancreatic necrosis virus. Nucleic Acids Res. 1976 Aug;3(8):1903–1924. doi: 10.1093/nar/3.8.1903. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doty P., McGill B. B., Rice S. A. THE PROPERTIES OF SONIC FRAGMENTS OF DEOXYRIBOSE NUCLEIC ACID. Proc Natl Acad Sci U S A. 1958 May;44(5):432–438. doi: 10.1073/pnas.44.5.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
- Loening U. E. The fractionation of high-molecular-weight ribonucleic acid by polyacrylamide-gel electrophoresis. Biochem J. 1967 Jan;102(1):251–257. doi: 10.1042/bj1020251. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Macdonald R. D., Yamamoto T. The structure of infectious pancreatic necrosis virus RNA. J Gen Virol. 1977 Feb;34(2):235–247. doi: 10.1099/0022-1317-34-2-235. [DOI] [PubMed] [Google Scholar]
- Müller H., Gissmann L. Mastomys natalensis papilloma virus (MnPV), the causative agent of epithelial proliferations: characterization of the virus particle. J Gen Virol. 1978 Nov;41(2):315–323. doi: 10.1099/0022-1317-41-2-315. [DOI] [PubMed] [Google Scholar]
- Nick H., Cursiefen D., Becht H. Structural and growth characteristics of infectious bursal disease virus. J Virol. 1976 Apr;18(1):227–234. doi: 10.1128/jvi.18.1.227-234.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nick H., Cursiefen D., Becht H. Structural and growth characteristics of two avian reoviruses. Arch Virol. 1975;48(3):261–269. doi: 10.1007/BF01317969. [DOI] [PubMed] [Google Scholar]
- Scholtissek C. Detection of an unstable RNA in chick fibroblasts after reduction of the UTP pool by glucosamine. Eur J Biochem. 1971 Dec;24(2):358–365. doi: 10.1111/j.1432-1033.1971.tb19694.x. [DOI] [PubMed] [Google Scholar]
- Scholtissek C., Harms E., Rohde W., Orlich M., Rott R. Correlation between RNA fragments of fowl plague virus and their corresponding gene functions. Virology. 1976 Oct 15;74(2):332–344. doi: 10.1016/0042-6822(76)90340-8. [DOI] [PubMed] [Google Scholar]
- Shatkin A. J. Inactivity of purified reovirus RNA as a template for E. coli polymerases in vitro. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1721–1728. doi: 10.1073/pnas.54.6.1721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Studier F. W. Analysis of bacteriophage T7 early RNAs and proteins on slab gels. J Mol Biol. 1973 Sep 15;79(2):237–248. doi: 10.1016/0022-2836(73)90003-x. [DOI] [PubMed] [Google Scholar]
- Watanabe Y., Graham A. F. Structural units of reovirus ribonucleic acid and their possible functional significance. J Virol. 1967 Aug;1(4):665–677. doi: 10.1128/jvi.1.4.665-677.1967. [DOI] [PMC free article] [PubMed] [Google Scholar]