Skip to main content
PMC home page
Journal of Virology logoLink to Journal of Virology
. 1979 Sep;31(3):845–848. doi: 10.1128/jvi.31.3.845-848.1979

Establishment of a lymphoblastoid cell line and isolation of an Epstein-Barr-related virus of gorilla origin.

R H Neubauer, H Rabin, B C Strnad, M Nonoyama, W A Nelson-Rees
PMCID: PMC353513  PMID: 92573

Abstract

A B-lymphoid cell line was established from a normal gorilla. The cells contained Epstein-Barr virus-related antigens, and herpesvirus particles were demonstrated by electron microscopy. DNA-DNA reassociation kinétics revealed 30 to 40% hybridization to Epstein-Barr virus with 50 genomes per cell. Examination of the viral nuclear antigen with gorilla sera showed this to be a unique isolate termed Herpesvirus gorilla. H. gorilla transformed gibbon B-lymphocytes in vitro.

Full text

PDF

Images in this article

846Image
on p.846

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Deinhardt F., Falk L., Wolfe L. G., Schudel A., Nonoyama M., Lai P., Lapin B., Yakovleva L. Susceptibility of marmosets to Epstein-Barr virus-like baboon herpesviruses. Primates Med. 1978;10:163–170. [PubMed] [Google Scholar]
  2. Gahmberg C. G., Hakomori S. I. External labeling of cell surface galactose and galactosamine in glycolipid and glycoprotein of human erythrocytes. J Biol Chem. 1973 Jun 25;248(12):4311–4317. [PubMed] [Google Scholar]
  3. Gerber P., Pritchett R. F., Kieff E. D. Antigens and DNA of a chimpanzee agent related to Epstein-Barr virus. J Virol. 1976 Sep;19(3):1090–1099. doi: 10.1128/jvi.19.3.1090-1099.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gilden R. V. Interrelationships among RNA tumor viruses and host cells. Adv Cancer Res. 1975;22:157–202. doi: 10.1016/s0065-230x(08)60177-3. [DOI] [PubMed] [Google Scholar]
  5. Miller D. A., Firschein I. L., Dev V. G., Tantravahi R., Miller O. J. The gorilla karyotype: chromosome lengths and polymorphisms. Cytogenet Cell Genet. 1974;13(6):536–550. doi: 10.1159/000130305. [DOI] [PubMed] [Google Scholar]
  6. Neubauer R. H., Rabin H., Brown A. M. Selective stimulation and differentiation of early antigens in lymphoblastoid cell lines producing Epstein-Barr-like viruses. J Gen Virol. 1978 Nov;41(2):295–301. doi: 10.1099/0022-1317-41-2-295. [DOI] [PubMed] [Google Scholar]
  7. Neubauer R. H., Rabin H., Strnad B. C., Lapin B. A., Yakovleva L. A., Indzie E. Antibody responses to Herpesvirus papio antigens in baboons with lymphoma. Int J Cancer. 1979 Feb;23(2):186–192. doi: 10.1002/ijc.2910230208. [DOI] [PubMed] [Google Scholar]
  8. Ohno S., Luka J., Falk L., Klein G. Detection of a nuclear, EBNA-type antigen in apparently EBNA-negative Herpesvirus papio (HVP)-transformed lymphoid lines by the acid-fixed nuclear binding technique. Int J Cancer. 1977 Dec 15;20(6):941–946. doi: 10.1002/ijc.2910200618. [DOI] [PubMed] [Google Scholar]
  9. Ohno S., Luka J., Lindahl T., Klein G. Identification of a purified complement-fixing antigen as the Epstein-Barr-virus determined nuclear antigen (EBNA) by its binding to metaphase chromosomes. Proc Natl Acad Sci U S A. 1977 Apr;74(4):1605–1609. doi: 10.1073/pnas.74.4.1605. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Rabin H., Benton C. V., Tainsky M. A., Rice N. R., Gilden R. V. Isolation and characterization of an endogenous type C virus of rhesus monkeys. Science. 1979 May 25;204(4395):841–842. doi: 10.1126/science.87013. [DOI] [PubMed] [Google Scholar]
  11. Rabin H., Neubauer R. H., Gonda M. A., Nelson-Rees W. A., Charman H. P., Valerio M. G. Spontaneous esophageal carcinoma and epithelial cell line of an adult rhesus monkey. Cancer Res. 1978 Oct;38(10):3310–3314. [PubMed] [Google Scholar]
  12. Rabin H., Neubauer R. H., Hopkins R. F., 3rd, Nonoyama M. Further characterization of a herpesvirus-positive orang-utan cell line and comparative aspects of in vitro transformation with lymphotropic old world primate herpesviruses. Int J Cancer. 1978 Jun 15;21(6):762–767. doi: 10.1002/ijc.2910210614. [DOI] [PubMed] [Google Scholar]
  13. Rasheed S., Rongey R. W., Bruszweski J., Nelson-Rees W. A., Rabin H., Neubauer R. H., Esra G., Gardner M. B. Establishment of a cell line with associated Epstein-Barr-like virus from a leukemic orangutan. Science. 1977 Oct 28;198(4315):407–409. doi: 10.1126/science.198878. [DOI] [PubMed] [Google Scholar]
  14. Reedman B. M., Klein G., Pope J. H., Walters M. K., Hilgers J., Singh S., Johansson B. Epstein-Barr virus-associated complement-fixing and nuclear antigens in Burkitt lymphoma biopsies. Int J Cancer. 1974 Jun 15;13(6):755–763. doi: 10.1002/ijc.2910130604. [DOI] [PubMed] [Google Scholar]
  15. Strnad B. C., Neubauer R. H., Rabin H. Biochemical identification of primate lymphoid cell-surface glycoproteins. Int J Cancer. 1979 Jan 15;23(1):76–81. doi: 10.1002/ijc.2910230114. [DOI] [PubMed] [Google Scholar]
  16. Tanaka A., Miyagi M., Yajima Y., Nonoyama M. Improved production of Epstein-Barr virus DNA for nucleic acid hybridization studies. Virology. 1976 Oct 1;74(1):81–85. doi: 10.1016/0042-6822(76)90130-6. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES