Skip to main content

Some NLM-NCBI services and products are experiencing heavy traffic, which may affect performance and availability. We apologize for the inconvenience and appreciate your patience. For assistance, please contact our Help Desk at info@ncbi.nlm.nih.gov.

Journal of Virology logoLink to Journal of Virology
. 1979 Oct;32(1):140–146. doi: 10.1128/jvi.32.1.140-146.1979

Mutant of herpes simplex virus type 2 with temperature-sensitive lesions affecting virion thermostability and DNase activity: identification of the lethal mutation and physical mapping of the nuc-lesion.

P Chartrand, M C Timbury, J Hay, H Moss
PMCID: PMC353536  PMID: 232166

Abstract

We had previously shown that a temperature-sensitive (ts) mutant of herpes simplex virus type 2 strain HG52, ts13, induced a heat-labile DNase activity in infected cells (B. Francke, H. Moss, M. C. Timbury, and J. Hay, J. Virol. 26:209-213, 1978). Earlier work indicated that the mutant also possessed temperature-sensitive infectivity (I. W. Halliburton and M. C. Timbury, J. Gen. Virol. 30:207-221, 1976). In this study temperature-stable revertants of ts13 have been isolated; examination of them revealed that ts13 is a double mutant, with genetically distinct temperature-sensitive lesions affecting nuclease activity and particle stability. The lethal mutation, in the cell system studied, is the latter. Revertants, which all maintain the nuclease lesion, grew well at a high temperature. Physical mapping of the nuclease lesion placed it between 0.12 and 0.21 (fractional length) on the virus genome, quite distant from the lethal mutation at 0.64 to 0.70.

Full text

PDF
140

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cortini R., Wilkie N. M. Physical maps for HSV type 2 DNA with five restriction endonucleases. J Gen Virol. 1978 May;39(2):259–280. doi: 10.1099/0022-1317-39-2-259. [DOI] [PubMed] [Google Scholar]
  2. Graham F. L., van der Eb A. J. A new technique for the assay of infectivity of human adenovirus 5 DNA. Virology. 1973 Apr;52(2):456–467. doi: 10.1016/0042-6822(73)90341-3. [DOI] [PubMed] [Google Scholar]
  3. Halliburton I. W., Timbury M. C. Temperature-sensitive mutants of herpes simplex virus type 2: description of three new complementation groups and studies on the inhibition of host cell DNA synthesis. J Gen Virol. 1976 Feb;30(2):207–221. doi: 10.1099/0022-1317-30-2-207. [DOI] [PubMed] [Google Scholar]
  4. Hoffmann P. J., Cheng Y. C. The deoxyribonuclease induced after infection of KB cells by herpes simplex virus type 1 or type 2. I. Purification and characterization of the enzyme. J Biol Chem. 1978 May 25;253(10):3557–3562. [PubMed] [Google Scholar]
  5. Jamieson A. T., Gentry G. A., Subak-Sharpe J. H. Induction of both thymidine and deoxycytidine kinase activity by herpes viruses. J Gen Virol. 1974 Sep;24(3):465–480. doi: 10.1099/0022-1317-24-3-465. [DOI] [PubMed] [Google Scholar]
  6. Morrison J. M., Keir H. M. A new DNA-exonuclease in cells infected with herpes virus: partial purification and properties of the enzyme. J Gen Virol. 1968 Dec;3(3):337–347. doi: 10.1099/0022-1317-3-3-337. [DOI] [PubMed] [Google Scholar]
  7. RUSSELL W. C. A sensitive and precise plaque assay for herpes virus. Nature. 1962 Sep 8;195:1028–1029. doi: 10.1038/1951028a0. [DOI] [PubMed] [Google Scholar]
  8. Stow N. D., Subak-Sharpe J. H., Wilkie N. M. Physical mapping of herpes simplex virus type 1 mutations by marker rescue. J Virol. 1978 Oct;28(1):182–192. doi: 10.1128/jvi.28.1.182-192.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Stow N. D., Wilkie N. M. An improved technique for obtaining enhanced infectivity with herpes simplex virus type 1 DNA. J Gen Virol. 1976 Dec;33(3):447–458. doi: 10.1099/0022-1317-33-3-447. [DOI] [PubMed] [Google Scholar]
  10. Wilkie N. M. The synthesis and substructure of herpesvirus DNA: the distribution of alkali-labile single strand interruptions in HSV-1 DNA. J Gen Virol. 1973 Dec;21(3):453–467. doi: 10.1099/0022-1317-21-3-453. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES