Table 1.
RcdA target list (SELEX-clos)
| Function | Left | S | Right | Function | No. | ||
|---|---|---|---|---|---|---|---|
| tfaX | > | > | appY | Transcription factor | 22 | ||
| Predicted transporter | ybjJ | < | > | rcdA | Transcription factor | 15 | |
| BLUE-EAL antirepressor | ycgF | < | > | ycgZ | RcsAB connector | 6 | |
| SOS cell division inhibitor | sulA | < | > | sxy | CRP coactivator | 3 | |
| pinE | > | > | mcrA | Restriction enzyme | 2 | ||
| gtrB | > | > | gtrS | Glucosyl transferase | 2 | ||
| CP4-6 conserved protein | yagK | < | < | yagL | 2 |
Genomic SELEX screening of RcdA-binding sequences was performed under the standard conditions (for details see text). RcdA-bound DNA fragments were subjected to SELEX-clos analysis. The clones independent obtained more than twice are shown (No., number of clones). All these fragments are located within spacer regions between “Right” and “Left” genes. The direction of transcription is shown by arrows.