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. 1979 Oct;32(1):339–344. doi: 10.1128/jvi.32.1.339-344.1979

Mappings of adenovirus type 7 cytoplasmic RNA species synthesized early in lytically infected cells and synthesized in transformed cells.

K Yoshida, K Sekikawa, K Fujinaga
PMCID: PMC353559  PMID: 541826

Abstract

Early virus-specific RNA synthesized in KB cells infected with adenovirus type 7 and virus-specific RNA synthesized in rat embryo cells (71JY1-2) transformed by the adenovirus type 7 HindIII-I.J fragment (left-hand 8.1% of the viral genome) have been mapped on the viral genome. About 25% of the viral genome, four discrete regions, two on each strand of the viral genome, are expressed as "early" mRNA. Almost similar regions in the left-hand 8.1% of the viral genome are transcribed both in KB cells at early times after infection and in 71JY1-2 cells.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Berk A. J., Sharp P. A. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids. Cell. 1977 Nov;12(3):721–732. doi: 10.1016/0092-8674(77)90272-0. [DOI] [PubMed] [Google Scholar]
  2. Berk A. J., Sharp P. A. Structure of the adenovirus 2 early mRNAs. Cell. 1978 Jul;14(3):695–711. doi: 10.1016/0092-8674(78)90252-0. [DOI] [PubMed] [Google Scholar]
  3. Chow L. T., Roberts J. M., Lewis J. B., Broker T. R. A map of cytoplasmic RNA transcripts from lytic adenovirus type 2, determined by electron microscopy of RNA:DNA hybrids. Cell. 1977 Aug;11(4):819–836. doi: 10.1016/0092-8674(77)90294-x. [DOI] [PubMed] [Google Scholar]
  4. Flint J. The topography and transcription of the adenovirus genome. Cell. 1977 Feb;10(2):153–166. doi: 10.1016/0092-8674(77)90211-2. [DOI] [PubMed] [Google Scholar]
  5. Flint S. J., Berget S. M., Sharp P. A. Adenovirus transcription. III. Mapping of viral RNA sequences in cells productively infected by adenovirus type 5. Virology. 1976 Jul 15;72(2):443–455. doi: 10.1016/0042-6822(76)90173-2. [DOI] [PubMed] [Google Scholar]
  6. Flint S. J., Gallimore P. H., Sharp P. A. Comparison of viral RNA sequences in adenovirus 2-transformed and lytically infected cells. J Mol Biol. 1975 Jul 25;96(1):47–68. doi: 10.1016/0022-2836(75)90181-3. [DOI] [PubMed] [Google Scholar]
  7. GREEN M., PINA M. BIOCHEMICAL STUDIES ON ADENOVIRUS MULTIPLICATION, VI. PROPERTIES OF HIGHLY PURIFIED TUMORIGENIC HUMAN ADENOVIRUSES AND THEIR DNA. Proc Natl Acad Sci U S A. 1964 Jun;51:1251–1259. doi: 10.1073/pnas.51.6.1251. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. GREEN M., PINA M. Biochemical studies on adenovirus multiplication. IV. Isolation, purification, and chemical analysis of adenovirus. Virology. 1963 May;20:199–207. doi: 10.1016/0042-6822(63)90157-0. [DOI] [PubMed] [Google Scholar]
  9. Hayward G. S. Gel electrophoretic separation of the complementary strands of bacteriophage DNA. Virology. 1972 Jul;49(1):342–344. doi: 10.1016/s0042-6822(72)80042-4. [DOI] [PubMed] [Google Scholar]
  10. Ortin J., Scheidtmann K. H., Greenberg R., Westphal M., Doerfler W. Transcription of the genome of adenovirus type 12. III. Maps of stable RNA from productively infected human cells and abortively infected and transformed hamster cells. J Virol. 1976 Nov;20(2):355–372. doi: 10.1128/jvi.20.2.355-372.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Pettersson U., Tibbetts C., Philipson L. Hybridization maps of early and late messenger RNA sequences on the adenovirus type 2 genome. J Mol Biol. 1976 Mar 15;101(4):479–501. doi: 10.1016/0022-2836(76)90241-2. [DOI] [PubMed] [Google Scholar]
  12. Sekikawa K., Fujinaga K. Cleavage maps of human adenovirus type 7 DNA by restriction endonuclease HindIII and EcoRI. Virology. 1977 Oct 15;82(2):509–512. doi: 10.1016/0042-6822(77)90022-8. [DOI] [PubMed] [Google Scholar]
  13. Sekikawa K., Shiroki K., Shimojo H., Ojima S., Fujinaga K. Transformation of a rat cell line by an adenovirus 7 DNA fragment. Virology. 1978 Jul 1;88(1):1–7. doi: 10.1016/0042-6822(78)90103-4. [DOI] [PubMed] [Google Scholar]
  14. Sharp P. A., Gallimore P. H., Flint S. J. Mapping of adenovirus 2 RNA sequences in lytically infected cells and transformed cell lines. Cold Spring Harb Symp Quant Biol. 1975;39(Pt 1):457–474. doi: 10.1101/sqb.1974.039.01.058. [DOI] [PubMed] [Google Scholar]
  15. Smiley J. R., Mak S. Adenovirus type 12-specific RNA sequences during productive infection of KB cells. J Virol. 1976 Jul;19(1):36–42. doi: 10.1128/jvi.19.1.36-42.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sussenbach J. S., Ellens D. J., Jansz H. S. Studies on the mechanism of replication of adenovirus DNA. II. The nature of single-stranded DNA in replicative intermediates. J Virol. 1973 Nov;12(5):1131–1138. doi: 10.1128/jvi.12.5.1131-1138.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Tibbetts C. Physical organization of subgroup B human adenovirus genomes. J Virol. 1977 Nov;24(2):564–579. doi: 10.1128/jvi.24.2.564-579.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Tsuchida N., Robin M. S., Green M. Viral RNA subunits in cells transformed by RNA tumor viruses. Science. 1972 Jun 30;176(4042):1418–1420. doi: 10.1126/science.176.4042.1418. [DOI] [PubMed] [Google Scholar]
  19. Wall R., Philipson L., Darnell J. E. Processing of adenovirus specific nuclear RNA during virus replication. Virology. 1972 Oct;50(1):27–34. doi: 10.1016/0042-6822(72)90342-x. [DOI] [PubMed] [Google Scholar]
  20. Zieve G., Penman S. Small RNA species of the HeLa cell: metabolism and subcellular localization. Cell. 1976 May;8(1):19–31. doi: 10.1016/0092-8674(76)90181-1. [DOI] [PubMed] [Google Scholar]

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