Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1979 Nov;32(2):420–428. doi: 10.1128/jvi.32.2.420-428.1979

Persistent vesicular stomatitis virus infection mediates base substitutions in viral RNA termini.

B L Semler, J J Holland
PMCID: PMC353573  PMID: 228067

Abstract

We have sequenced (via a product RNA) the 3' RNA terminus of a defective interfering particle that was generated from the standard virus isolated from a culture of BHK-21 cells persistently infected with vesicular stomatitis virus for over 5 years. By hybridization and RNA sequencing, seven mutations were identified in the 46 nucleotides at the terminus of this defective-interfering-particle RNA. It is likely that these mutations are a reflection of altered protein-nucleic acid interactions that the virus has evolved to maintain its persistently infected carrier state in vitro.

Full text

PDF
420

Images in this article

422Image
on p.422
424Image
on p.424
425Image
on p.425

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ball L. A., White C. N. Order of transcription of genes of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1976 Feb;73(2):442–446. doi: 10.1073/pnas.73.2.442. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltimore D., Huang A. S., Stampfer M. Ribonucleic acid synthesis of vesicular stomatitis virus, II. An RNA polymerase in the virion. Proc Natl Acad Sci U S A. 1970 Jun;66(2):572–576. doi: 10.1073/pnas.66.2.572. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Banerjee A. D., Abraham G., Colonno R. J. Vesicular stomatitis virus: mode of transcription. J Gen Virol. 1977 Jan;34(1):1–8. doi: 10.1099/0022-1317-34-1-1. [DOI] [PubMed] [Google Scholar]
  4. Colonno R. J., Banerjee A. K. A unique RNA species involved in initiation of vesicular stomatitis virus RNA transcription in vitro. Cell. 1976 Jun;8(2):197–204. doi: 10.1016/0092-8674(76)90003-9. [DOI] [PubMed] [Google Scholar]
  5. Colonno R. J., Banerjee A. K. Complete nucleotide sequence of the leader RNA synthesized in vitro by vesicular stomatitis virus. Cell. 1978 Sep;15(1):93–101. doi: 10.1016/0092-8674(78)90085-5. [DOI] [PubMed] [Google Scholar]
  6. Doyle M., Holland J. J. Prophylaxis and immunization in mice by use of virus-free defective T particles to protect against intracerebral infection by vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1973 Jul;70(7):2105–2108. doi: 10.1073/pnas.70.7.2105. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Emerson S. U., Dierks P. M., Parsons J. T. In vitro synthesis of a unique RNA species by a T particle of vesicular stomatitis virus. J Virol. 1977 Sep;23(3):708–716. doi: 10.1128/jvi.23.3.708-716.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Holland J. J., Grabau E. A., Jones C. L., Semler B. L. Evolution of multiple genome mutations during long-term persistent infection by vesicular stomatitis virus. Cell. 1979 Mar;16(3):495–504. doi: 10.1016/0092-8674(79)90024-2. [DOI] [PubMed] [Google Scholar]
  9. Holland J. J., Villarreal L. P. Persistent noncytocidal vesicular stomatitis virus infections mediated by defective T particles that suppress virion transcriptase. Proc Natl Acad Sci U S A. 1974 Aug;71(8):2956–2960. doi: 10.1073/pnas.71.8.2956. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Holland J. J., Villarreal L. P., Welsh R. M., Oldstone M. B., Kohne D., Lazzarini R., Scolnick E. Long-term persistent vesicular stomatitis virus and rabies virus infection of cells in vitro. J Gen Virol. 1976 Nov;33(2):193–211. doi: 10.1099/0022-1317-33-2-193. [DOI] [PubMed] [Google Scholar]
  11. Huang A. S. Defective interfering viruses. Annu Rev Microbiol. 1973;27:101–117. doi: 10.1146/annurev.mi.27.100173.000533. [DOI] [PubMed] [Google Scholar]
  12. Keene J. D., Schubert M., Lazzarini R. A., Rosenberg M. Nucleotide sequence homology at the 3' termini of RNA from vesicular stomatitis virus and its defective interfering particles. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3225–3229. doi: 10.1073/pnas.75.7.3225. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kolakofsky D. Isolation and characterization of Sendai virus DI-RNAs. Cell. 1976 Aug;8(4):547–555. doi: 10.1016/0092-8674(76)90223-3. [DOI] [PubMed] [Google Scholar]
  14. Lazzarini R. A., Weber G. H., Johnson L. D., Stamminger G. M. Covalently linked message and anti-message (genomic) RNA from a defective vesicular stomatitis virus particle. J Mol Biol. 1975 Sep 25;97(3):289–307. doi: 10.1016/s0022-2836(75)80042-8. [DOI] [PubMed] [Google Scholar]
  15. Leppert M., Kort L., Kolakofsky D. Further characterization of Sendai virus DI-RNAs: a model for their generation. Cell. 1977 Oct;12(2):539–552. doi: 10.1016/0092-8674(77)90130-1. [DOI] [PubMed] [Google Scholar]
  16. Maxam A. M., Gilbert W. A new method for sequencing DNA. Proc Natl Acad Sci U S A. 1977 Feb;74(2):560–564. doi: 10.1073/pnas.74.2.560. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Perrault J. Cross-linked double-stranded RNA from a defective vesicular stomatitis virus particle. Virology. 1976 Apr;70(2):360–371. doi: 10.1016/0042-6822(76)90278-6. [DOI] [PubMed] [Google Scholar]
  18. Perrault J., Kingsbury D. T. Inhibitor of vesicular stomatitis virus transcriptase in purified virions. Nature. 1974 Mar 1;248(5443):45–47. doi: 10.1038/248045a0. [DOI] [PubMed] [Google Scholar]
  19. Perrault J., Leavitt R. W. Inverted complementary terminal sequences in single-stranded RNAs and snap-back RNAs from vesicular stomatitis defective interfering particles. J Gen Virol. 1978 Jan;38(1):35–50. doi: 10.1099/0022-1317-38-1-35. [DOI] [PubMed] [Google Scholar]
  20. Reichmann M. E., Schnitzlein W. M. Defective interfering particles of rhabdoviruses. Curr Top Microbiol Immunol. 1979;86:123–168. doi: 10.1007/978-3-642-67341-2_4. [DOI] [PubMed] [Google Scholar]
  21. Reichmann M. E., Villarreal L. P., Kohne D., Lesnaw J., Holland J. J. RNA polymerase activity and poly(A) synthesizing activity in defective T particles of vesicular stomatitis virus. Virology. 1974 Mar;58(1):240–249. doi: 10.1016/0042-6822(74)90158-5. [DOI] [PubMed] [Google Scholar]
  22. Richardson C. C. Phosphorylation of nucleic acid by an enzyme from T4 bacteriophage-infected Escherichia coli. Proc Natl Acad Sci U S A. 1965 Jul;54(1):158–165. doi: 10.1073/pnas.54.1.158. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Rima R. K., Martin S. J. Persistent infection of tissue culture cells by RNA viruses. Med Microbiol Immunol. 1976 Jun 1;162(2):89–119. doi: 10.1007/BF02121320. [DOI] [PubMed] [Google Scholar]
  24. Schubert M., Keene J. D., Lazzarini R. A., Emerson S. U. The complete sequence of a unique RNA species synthesized by a DI particle of VSV. Cell. 1978 Sep;15(1):103–112. doi: 10.1016/0092-8674(78)90086-7. [DOI] [PubMed] [Google Scholar]
  25. Semler B. L., Perrault J., Abelson J., Holland J. J. Sequence of a RNA templated by the 3'-OH RNA terminus of defective interfering particles of vesicular stomatitis virus. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4704–4708. doi: 10.1073/pnas.75.10.4704. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Simoncsits A., Brownlee G. G., Brown R. S., Rubin J. R., Guilley H. New rapid gel sequencing method for RNA. Nature. 1977 Oct 27;269(5631):833–836. doi: 10.1038/269833a0. [DOI] [PubMed] [Google Scholar]
  27. Stampfer M., Baltimore D., Huang A. S. Absence of interference during high-multiplicity infection by clonally purified vesicular stomatitis virus. J Virol. 1971 Mar;7(3):409–411. doi: 10.1128/jvi.7.3.409-411.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Villarreal L. P., Holland J. J. RNA synthesis in BHK 21 cells persistently infected with vesicular stomatitis virus and rabies virus. J Gen Virol. 1976 Nov;33(2):213–224. doi: 10.1099/0022-1317-33-2-213. [DOI] [PubMed] [Google Scholar]
  29. WALKER D. L. THE VIRAL CARRIER STATE IN ANIMAL CELL CULTURES. Prog Med Virol. 1964;6:111–148. [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES