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Journal of Virology logoLink to Journal of Virology
. 1979 Nov;32(2):676–678. doi: 10.1128/jvi.32.2.676-678.1979

Production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2.

M Zweig, C J Heilman Jr, H Rabin, R F Hopkins 3rd, R H Neubauer, B Hampar
PMCID: PMC353600  PMID: 91687

Abstract

We prepared mouse hybrid cell lines which produced antibodies against herpes simplex virus type 1 and 2 nucleocapsids. Cell lines 1D4 and 3E1, respectively, secreted immunoglobulin G1 herpes simplex virus type 1 and immunoglobulin G1 herpes simplex virus type 2 antibodies which immunoprecipitated proteins designated p40 and p45 from homologous nucleocapsid preparations but precipitated no proteins from heterologous preparations. In contrast, guinea pig antisera prepared against either herpes simplex virus type 1 or 2 p40 precipitated p40 and p45 from both homologous and heterologous preparations. These findings suggest that p40 and p45 possess similar antigenic determinants and that the monoclonal antibodies that were tested reacted preferentially with the homologous determinants.

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Selected References

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