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. 1979 Nov;32(2):688–691. doi: 10.1128/jvi.32.2.688-691.1979

Cell-free synthesis of measles virus proteins.

J Sprague, L J Eron, A S Seifried, J B Milstien
PMCID: PMC353603  PMID: 501804

Abstract

Polyadenylated mRNA extracted from cytoplasm of measles virus-infected Vero cells was translated in a cell-free system. Three of the polypeptides obtained corresponded to nucleocapsid protein, phosphoprotein, and membrane protein of measles virions. A fourth polypeptide, present in measles virus-infected cells, could be generated by addition of Vero cytoplasmic extract and was identified as a cleavage product of the nucleocapsid protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bussell R. H., Waters D. J., Seals M. K. Measles, canine distemper and respiratory syncytial virions and nucleocapsids. A comparative study of their structure, polypeptide and nucleic acid composition. Med Microbiol Immunol. 1974;160(2-3):105–124. doi: 10.1007/BF02121718. [DOI] [PubMed] [Google Scholar]
  2. Content J. Cell-free translation of influenza virus mRNA. J Virol. 1976 May;18(2):604–618. doi: 10.1128/jvi.18.2.604-618.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Eron L., Callahan R., Westphal H. Cell-free synthesis of adenovirus coat proteins. J Biol Chem. 1974 Oct 10;249(19):6331–6338. [PubMed] [Google Scholar]
  4. Etkind P. R., Krug R. M. Purification of influenza viral complementary RNA: its genetic content and activity in wheat germ cell-free extracts. J Virol. 1975 Dec;16(6):1464–1475. doi: 10.1128/jvi.16.6.1464-1475.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Hall W. W., Kiessling W., ter Meulen V. Membrane proteins of subacute sclerosing panencephalitis and measles viruses. Nature. 1978 Mar 30;272(5652):460–462. doi: 10.1038/272460a0. [DOI] [PubMed] [Google Scholar]
  6. Hall W. W., ter Meulen V. RNA homology between subacute sclerosing panencephalitis and measles viruses. Nature. 1976 Dec 2;264(5585):474–477. doi: 10.1038/264474a0. [DOI] [PubMed] [Google Scholar]
  7. Horta-Barbosa L., Fuccillo D. A., Hamilton R., Traub R., Ley A., Sever J. L. Some characteristics of SSPE measles virus. Proc Soc Exp Biol Med. 1970 May;134(1):17–21. doi: 10.3181/00379727-134-34718. [DOI] [PubMed] [Google Scholar]
  8. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  9. Lamb R. A., Choppin P. W. Determination by peptide mapping of the unique polypeptides in Sendai virions and infected cells. Virology. 1978 Feb;84(2):469–478. doi: 10.1016/0042-6822(78)90263-5. [DOI] [PubMed] [Google Scholar]
  10. Mountcastle W. E., Compans R. W., Caliguiri L. A., Choppin P. W. Nucleocapsid protein subunits of simian virus 5, Newcastle disease virus, and Sendai virus. J Virol. 1970 Nov;6(5):677–684. doi: 10.1128/jvi.6.5.677-684.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. O'Farrell P. Z., Goodman H. M., O'Farrell P. H. High resolution two-dimensional electrophoresis of basic as well as acidic proteins. Cell. 1977 Dec;12(4):1133–1141. doi: 10.1016/0092-8674(77)90176-3. [DOI] [PubMed] [Google Scholar]
  12. Richey M. B., Palese P. In vitro translation of influenza virus messenger RNAs. Virology. 1976 Jul 15;72(2):410–420. doi: 10.1016/0042-6822(76)90170-7. [DOI] [PubMed] [Google Scholar]
  13. Roberts B. E., Paterson B. M. Efficient translation of tobacco mosaic virus RNA and rabbit globin 9S RNA in a cell-free system from commercial wheat germ. Proc Natl Acad Sci U S A. 1973 Aug;70(8):2330–2334. doi: 10.1073/pnas.70.8.2330. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rozenblatt S., Gorecki M., Shure H., Prives C. L. Characterization of measles virus-specific proteins synthesized in vivo and in vitro from acutely and persistently infected cells. J Virol. 1979 Mar;29(3):1099–1016. doi: 10.1128/jvi.29.3.1099-1106.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Schluederberg A. Measles virus RNA. Biochem Biophys Res Commun. 1971 Mar 19;42(6):1012–1015. doi: 10.1016/0006-291x(71)90004-0. [DOI] [PubMed] [Google Scholar]
  16. Seifried A. S., Albrecht P., Milstien J. B. Characterization of an RNA-dependent RNA polymerase activity associated with measles virus. J Virol. 1978 Mar;25(3):781–787. doi: 10.1128/jvi.25.3.781-787.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Stallcup K. C., Wechsler S. L., Fields B. N. Purification of measles virus and characterization of subviral components. J Virol. 1979 Apr;30(1):166–176. doi: 10.1128/jvi.30.1.166-176.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Waters D. J., Bussell R. H. Polypeptide composition of measles and canine distemper viruses. Virology. 1973 Oct;55(2):554–557. doi: 10.1016/0042-6822(73)90202-x. [DOI] [PubMed] [Google Scholar]

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