Abstract
By electron microscopic studies, the adsorption and release of nonlytic, cytocidal mycoplasma virus MVL3, which infects ACholeplasma laidlawii cells, have been examined. The MVL3 virion has a polyhedral head, collar, short tail, and tail fibers and contains linear double-stranded DNA. Adsorbed MVL3 virus showed a temperature-dependent clustering or capping on the mycoplasma cell membrane. During infection, a number of virus-cell membrane-related structures were observed, suggesting a general model in which MVL3-infected cells release progeny virions in membrane vesicles. These vesicles must then break down to release MVL3 particles.
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