Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Nov;36(2):337–352. doi: 10.1128/jvi.36.2.337-352.1980

Unique species of mRNA from adenovirus type 7 early region 1 in cells transformed by adenovirus type 7 DNA fragment.

K Yoshida, K Fujinaga
PMCID: PMC353650  PMID: 6253660

Abstract

Adenovirus type 7 (Ad7) early region 1 mRNA species transcribed in rat cell lines transformed by the HindIII-I . J fragment (the left 7.8% of the viral genome) and in human KB cells infected with Ad7 were mapped on the viral genome, using S1 nuclease gel and diazobenzyloxymethyl paper hybridization techniques. At the early stage of productive infection, two mRNA's (950 and 840 nucleotides long) with the common 5' and 3' ends but different internal splicings were mapped from region 1A (map units 1.4 to 4.3), and one mRNA (2,310 nucleotides long, with the internal splicing between map units 9.9 to 10.1) was mapped from region 1B (map units 4.6 to 11.4). At the late stage, these early spliced mRNA's were also found and at least three additional Ad7 mRNA's were identified: 700-nucleotide-long mRNA in region 1A; and 1,100- and nucleotide-long mRNA's in region 1B. In transformed rat cell lines, two early region 1A mRNA's (950 and 840 nucleotides long) were also transcribed. Surprisingly, in addition, several unique Ad7 mRNA's, not found in productivity infected cells, were identified in all of the transformed cell lines. Their molecular sizes and coding sequences varied in individual cell lines. However, these mRNA's had the 5' end-proximal portion in region 1B and the 3' end-proximal portion in region 1A, these portions being transcribed by extending from region 1B to 1A on viral DNA fragments joined in a tandem array in transformed cells.

Full text

PDF
337

Images in this article

340Image
on p.340
341Image
on p.341
344Image
on p.344
346Image
on p.346
348Image
on p.348

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Alwine J. C., Kemp D. J., Stark G. R. Method for detection of specific RNAs in agarose gels by transfer to diazobenzyloxymethyl-paper and hybridization with DNA probes. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5350–5354. doi: 10.1073/pnas.74.12.5350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berk A. J., Lee F., Harrison T., Williams J., Sharp P. A. Pre-early adenovirus 5 gene product regulates synthesis of early viral messenger RNAs. Cell. 1979 Aug;17(4):935–944. doi: 10.1016/0092-8674(79)90333-7. [DOI] [PubMed] [Google Scholar]
  3. Berk A. J., Sharp P. A. Structure of the adenovirus 2 early mRNAs. Cell. 1978 Jul;14(3):695–711. doi: 10.1016/0092-8674(78)90252-0. [DOI] [PubMed] [Google Scholar]
  4. Casey J., Davidson N. Rates of formation and thermal stabilities of RNA:DNA and DNA:DNA duplexes at high concentrations of formamide. Nucleic Acids Res. 1977;4(5):1539–1552. doi: 10.1093/nar/4.5.1539. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Chinnadural G., Fujinaga K., Rho M. H., van der Eb A. J., Green M. Transcription of the transforming region of adenovirus type 5 in a rat cell line transformed by a small restriction fragment. J Virol. 1978 Dec;28(3):1011–1014. doi: 10.1128/jvi.28.3.1011-1014.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chow L. T., Broker T. R., Lewis J. B. Complex splicing patterns of RNAs from the early regions of adenovirus-2. J Mol Biol. 1979 Oct 25;134(2):265–303. doi: 10.1016/0022-2836(79)90036-6. [DOI] [PubMed] [Google Scholar]
  7. Dijkema R., Dekker B. M., Van Ormondt H. The nucleotide sequence of the transforming BglII-H fragment of adenovirus type 7 DNA. Gene. 1980 Apr;9(1-2):141–156. doi: 10.1016/0378-1119(80)90172-9. [DOI] [PubMed] [Google Scholar]
  8. Dijkema R., Dekker B. M., van der Feltz M. J., van der Eb A. J. Transformation of primary rat kidney cells by DNA fragments of weakly oncogenic adenoviruses. J Virol. 1979 Dec;32(3):943–950. doi: 10.1128/jvi.32.3.943-950.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Flint J. The topography and transcription of the adenovirus genome. Cell. 1977 Feb;10(2):153–166. doi: 10.1016/0092-8674(77)90211-2. [DOI] [PubMed] [Google Scholar]
  10. Flint S. J., Berget S. M., Sharp P. A. Adenovirus transcription. III. Mapping of viral RNA sequences in cells productively infected by adenovirus type 5. Virology. 1976 Jul 15;72(2):443–455. doi: 10.1016/0042-6822(76)90173-2. [DOI] [PubMed] [Google Scholar]
  11. Jones N., Shenk T. An adenovirus type 5 early gene function regulates expression of other early viral genes. Proc Natl Acad Sci U S A. 1979 Aug;76(8):3665–3669. doi: 10.1073/pnas.76.8.3665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Mackey J. K., Brackmann K. H., Green M. R., Green M. Preparation and characterization of highly radioactive in vitro labeled adenovirus DNA and DNA restriction fragments. Biochemistry. 1977 Oct 4;16(20):4478–4483. doi: 10.1021/bi00639a023. [DOI] [PubMed] [Google Scholar]
  13. McDonell M. W., Simon M. N., Studier F. W. Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. J Mol Biol. 1977 Feb 15;110(1):119–146. doi: 10.1016/s0022-2836(77)80102-2. [DOI] [PubMed] [Google Scholar]
  14. Perricaudet M., Akusjärvi G., Virtanen A., Pettersson U. Structure of two spliced mRNAs from the transforming region of human subgroup C adenoviruses. Nature. 1979 Oct 25;281(5733):694–696. doi: 10.1038/281694a0. [DOI] [PubMed] [Google Scholar]
  15. Sawada Y., Ojima S., Shimojo H., Shiroki K., Fujinaga K. Transforming DNA sequences in rat cells transformed by DNA fragments of highly oncogenic human adenovirus type 12. J Virol. 1979 Nov;32(2):379–385. doi: 10.1128/jvi.32.2.379-385.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sekikawa K., Fujinaga K. Cleavage maps of human adenovirus type 7 DNA by restriction endonuclease HindIII and EcoRI. Virology. 1977 Oct 15;82(2):509–512. doi: 10.1016/0042-6822(77)90022-8. [DOI] [PubMed] [Google Scholar]
  17. Sekikawa K., Shiroki K., Shimojo H., Ojima S., Fujinaga K. Transformation of a rat cell line by an adenovirus 7 DNA fragment. Virology. 1978 Jul 1;88(1):1–7. doi: 10.1016/0042-6822(78)90103-4. [DOI] [PubMed] [Google Scholar]
  18. Shiroki K., Handa H., Shimojo H., Yano S., Ojima S., Fujinaga K. Establishment and characterization of rat cell lines transformed by restriction endonuclease fragments of adenovirus 12 DNA. Virology. 1977 Oct 15;82(2):462–471. doi: 10.1016/0042-6822(77)90019-8. [DOI] [PubMed] [Google Scholar]
  19. Shiroki K., Shimojo H., Sawada Y., Uemizu Y., Fujinaga K. Incomplete transformation of rat cells by a small fragment of adenovirus 12 DNA. Virology. 1979 May;95(1):127–136. doi: 10.1016/0042-6822(79)90407-0. [DOI] [PubMed] [Google Scholar]
  20. Spector D. J., McGrogan M., Raskas H. J. Regulation of the appearance of cytoplasmic RNAs from region 1 of the adenovirus 2 genome. J Mol Biol. 1978 Dec 15;126(3):395–414. doi: 10.1016/0022-2836(78)90048-7. [DOI] [PubMed] [Google Scholar]
  21. Takahashi H., Shimizu M., Saito H., Ikeda Y., Sugisaki H. A new site-specific endonuclease from Streptomyces lavendulae (SlaI). Gene. 1979 Jan;5(1):9–18. doi: 10.1016/0378-1119(79)90089-1. [DOI] [PubMed] [Google Scholar]
  22. Van Ormondt H., Maat J., De Waard A., Van der Eb A. J. The nucleotide sequence of the transforming HpaI-E fragment of adenovirus type 5 DNA. Gene. 1978 Dec;4(4):309–328. doi: 10.1016/0378-1119(78)90048-3. [DOI] [PubMed] [Google Scholar]
  23. Van der Eb A. J., Mulder C., Graham F. L., Houweling A. Transformation with specific fragments of adenovirus DNAs. I. Isolation of specific fragments with transforming activity of adenovirus 2 and 5 DNA. Gene. 1977;2(3-4):115–132. doi: 10.1016/0378-1119(77)90012-9. [DOI] [PubMed] [Google Scholar]
  24. Wellauer P. K., Dawid I. B. Secondary structure maps of RNA: processing of HeLa ribosomal RNA. Proc Natl Acad Sci U S A. 1973 Oct;70(10):2827–2831. doi: 10.1073/pnas.70.10.2827. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Wilson M. C., Fraser N. W., Darnell J. E., Jr Mapping of RNA initiation sites by high doses of uv irradiation: evidence for three independent promoters within the left 11% of the Ad-2 genome. Virology. 1979 Apr 15;94(1):175–184. doi: 10.1016/0042-6822(79)90447-1. [DOI] [PubMed] [Google Scholar]
  26. Wilson M. C., Sawicki S. G., Salditt-Georgieff M., Darnell J. E. Adenovirus type 2 mRNA in transformed cells: map positions and difference in transport time. J Virol. 1978 Jan;25(1):97–103. doi: 10.1128/jvi.25.1.97-103.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Yoshida K., Sekikawa K., Fujinaga K. Mappings of adenovirus type 7 cytoplasmic RNA species synthesized early in lytically infected cells and synthesized in transformed cells. J Virol. 1979 Oct;32(1):339–344. doi: 10.1128/jvi.32.1.339-344.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Zieve G., Penman S. Small RNA species of the HeLa cell: metabolism and subcellular localization. Cell. 1976 May;8(1):19–31. doi: 10.1016/0092-8674(76)90181-1. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES