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. 1980 Nov;36(2):353–360. doi: 10.1128/jvi.36.2.353-360.1980

Intracellular forms of simian virus 40 nucleoprotein complexes. III. Study of histone modifications.

M Coca-Prados, G Vidali, M T Hsu
PMCID: PMC353651  PMID: 6253661

Abstract

The modification patterns of histones present in various forms of intracellular simian virus 40 nucleoprotein complexes were analyzed by acetic acid-urea-polyacrylamide gel electrophoresis. The results showed that different viral nucleoprotein complexes contain different histone patterns. Simian virus 40 chromatin, which contains the activities for the synthesis of viral RNA and DNA, exhibits a histone modification pattern similar to that of the host chromatin. However, virion assembly intermediates and mature virions contain highly modified histones. Pulse-chase experiments with [3H]lysine showed that the newly incorporated histones in the virion assembly intermediates were already highly modified. The majority of in vivo acetylation activity of histones occurred on the 70S simian virus 40 chromatin as analyzed by pulse-labeling with [3H]acetate. These results and our previous analysis of the virion assembly pathway suggest that three stages are involved in the packaging of simian virus 40 chromatin into the mature virion: (i) modification of histones, (ii) accumulation of capsid protein around the chromatin with highly modified histones, and (iii) organization of capsid proteins into salt-resistant shells. The role of histone modification in virion assembly is discussed.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chen Y. H., MacGregor J. P., Goldstein D. A., Hall M. R. Histone modifications in simian virus 40 and in nucleoprotein complexes containing supercoiled viral DNA. J Virol. 1979 Apr;30(1):218–224. doi: 10.1128/jvi.30.1.218-224.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chestier A., Yaniv M. Rapid turnover of acetyl groups in the four core histones of simian virus 40 minichromosomes. Proc Natl Acad Sci U S A. 1979 Jan;76(1):46–50. doi: 10.1073/pnas.76.1.46. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coca-Prados M., Hsu M. T. Intracellular forms of simian virus 40 nucleoprotein complexes. II. Biochemical and electron microscopic analysis of simian virus 40 virion assembly. J Virol. 1979 Jul;31(1):199–208. doi: 10.1128/jvi.31.1.199-208.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Crémisi C., Chestier A., Yaniv M. Preferential association of newly synthesized histones with replicating SV40 DNA. Cell. 1977 Dec;12(4):947–951. doi: 10.1016/0092-8674(77)90159-3. [DOI] [PubMed] [Google Scholar]
  5. Fareed G. C., Davoli D. Molecular biology of papovaviruses. Annu Rev Biochem. 1977;46:471–522. doi: 10.1146/annurev.bi.46.070177.002351. [DOI] [PubMed] [Google Scholar]
  6. Fernandez-Munoz R., Coca-Prados M., Hsu M. T. Intracellular forms of simian virus 40 nucleoprotein complexes. I. Methods of isolation and characterization in CV-1 cells. J Virol. 1979 Feb;29(2):612–623. doi: 10.1128/jvi.29.2.612-623.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Jackson V., Shires A., Tanphaichitr N., Chalkley R. Modifications to histones immediately after synthesis. J Mol Biol. 1976 Jun 25;104(2):471–483. doi: 10.1016/0022-2836(76)90282-5. [DOI] [PubMed] [Google Scholar]
  8. La Bella F., Vesco C. Late modifications of simian virus 40 chromatin during the lytic cycle occur in an immature form of virion. J Virol. 1980 Mar;33(3):1138–1150. doi: 10.1128/jvi.33.3.1138-1150.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. La Bella F., Vidali G., Vesco C. Histone acetylation in CV-1 cells infected with simian virus 40. Virology. 1979 Jul 30;96(2):564–575. doi: 10.1016/0042-6822(79)90112-0. [DOI] [PubMed] [Google Scholar]
  10. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  11. MacGregor J. P., Chen Y. H., Goldstein D. A., Hall M. R. Properties of the histones associated with simian virus 40 replicative form nucleoprotein complex. Biochem Biophys Res Commun. 1978 Aug 14;83(3):814–821. doi: 10.1016/0006-291x(78)91467-5. [DOI] [PubMed] [Google Scholar]
  12. PHILLIPS D. M. The presence of acetyl groups of histones. Biochem J. 1963 May;87:258–263. doi: 10.1042/bj0870258. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Panyim S., Chalkley R. High resolution acrylamide gel electrophoresis of histones. Arch Biochem Biophys. 1969 Mar;130(1):337–346. doi: 10.1016/0003-9861(69)90042-3. [DOI] [PubMed] [Google Scholar]
  14. Tan K. B. Histones: metabolism in simian virus 40-infected cells and incorporation into virions. Proc Natl Acad Sci U S A. 1977 Jul;74(7):2805–2809. doi: 10.1073/pnas.74.7.2805. [DOI] [PMC free article] [PubMed] [Google Scholar]

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