Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Nov;36(2):421–428. doi: 10.1128/jvi.36.2.421-428.1980

Factors which disorganize microtubules or microfilaments increase the frequency of cell transformation by polyoma virus.

R Seif
PMCID: PMC353658  PMID: 6253667

Abstract

Griseofulvin, 12-O-tetradecanoyl phorbol-13-acetate, melittin, epidermal growth factor, vinblastine, cytochalasin B, podophyllotoxin, colcemid, and colchicine were unable to transform cells but could increase from 8- to 40-fold the frequency of cell transformation by polyoma virus. The 3T3-like cells were resting at confluence and were exposed to the drug only during the 1st week after viral infection. Griseofulvin, a tumor promoter, reduced or increased the frequency of transformation depending on the dose with which the infected cells were treated. The antitumor activity of tumor promoters is discussed.

Full text

PDF
421

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BARICH L. L., SCHWARZ J., BARICH D. Oral griseofulvin: a cocarcinogenic agent to methylcholanthrene induced cutaneous tumors. Cancer Res. 1962 Jan;22:53–55. [PubMed] [Google Scholar]
  2. Colburn N. H., Former B. F., Nelson K. A., Yuspa S. H. Tumour promoter induces anchorage independence irreversibly. Nature. 1979 Oct 18;281(5732):589–591. doi: 10.1038/281589a0. [DOI] [PubMed] [Google Scholar]
  3. Defendi V., Stoker M. G. General polyploid produced by cytochalasin B. Nat New Biol. 1973 Mar 7;242(114):24–26. doi: 10.1038/newbio242024a0. [DOI] [PubMed] [Google Scholar]
  4. Fiers W., Contreras R., Haegemann G., Rogiers R., Van de Voorde A., Van Heuverswyn H., Van Herreweghe J., Volckaert G., Ysebaert M. Complete nucleotide sequence of SV40 DNA. Nature. 1978 May 11;273(5658):113–120. doi: 10.1038/273113a0. [DOI] [PubMed] [Google Scholar]
  5. Friedmann T., Esty A., LaPorte P., Deininger P. The nucleotide sequence and genome organization of the polyoma early region: extensive nucleotide and amino acid homology with SV40. Cell. 1979 Jul;17(3):715–724. doi: 10.1016/0092-8674(79)90278-2. [DOI] [PubMed] [Google Scholar]
  6. Graessmann A., Graessmann M., Tjian R., Topp W. C. Simian virus 40 small-t protein is required for loss of actin cable networks in rat cells. J Virol. 1980 Mar;33(3):1182–1191. doi: 10.1128/jvi.33.3.1182-1191.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Hart I. R., Raz A., Fidler I. J. Effect of cytoskeleton-disrupting agents on the metastatic behavior of melanoma cells. J Natl Cancer Inst. 1980 Apr;64(4):891–900. [PubMed] [Google Scholar]
  8. Hirai K., Defendi V., Diamond L. Enhancement of simian virus 40 transformation and integration by 4-nitroquinoline 1-oxide. Cancer Res. 1974 Dec;34(12):3497–3500. [PubMed] [Google Scholar]
  9. Howard H. T., Howden M. E. Antitumor activity in Pimelia simplex. Cancer Chemother Rep. 1975 May-Jun;59(3):585–586. [PubMed] [Google Scholar]
  10. Huang C. C., Hou Y., Wang J. J. Effects of a new antitumor agent, epipodophyllotoxin, on growth and chromosomes in human hematopoietic cell lines. Cancer Res. 1973 Dec;33(12):3123–3129. [PubMed] [Google Scholar]
  11. JOHNSON I. S., ARMSTRONG J. G., GORMAN M., BURNETT J. P., Jr THE VINCA ALKALOIDS: A NEW CLASS OF ONCOLYTIC AGENTS. Cancer Res. 1963 Sep;23:1390–1427. [PubMed] [Google Scholar]
  12. Kinsella A. R., Radman M. Tumor promoter induces sister chromatid exchanges: relevance to mechanisms of carcinogenesis. Proc Natl Acad Sci U S A. 1978 Dec;75(12):6149–6153. doi: 10.1073/pnas.75.12.6149. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kupchan S. M., Baxter R. L. Mezerein: antileukemic principle isolated from Daphne mezereum L. Science. 1975 Feb 21;187(4177):652–653. doi: 10.1126/science.1114315. [DOI] [PubMed] [Google Scholar]
  14. Kupchan S. M., Uchida I., Branfman A. R., Dailey R. G., Jr, Fei B. Y. Antileukemic principles isolated from euphorbiaceae plants. Science. 1976 Feb 13;191(4227):571–572. doi: 10.1126/science.1251193. [DOI] [PubMed] [Google Scholar]
  15. Larizza L., Simoni G., Tredici F., De Carli L. Griseofulvin: a potential agent of chromosomal segregation in cultured cells. Mutat Res. 1974 Oct;25(1):123–130. doi: 10.1016/0027-5107(74)90224-3. [DOI] [PubMed] [Google Scholar]
  16. Loveday K. S., Latt S. A. The effect of a tumor promotor, 12-O-tetradecanoyl-phorbol-13-acetate (TPA), on sister-chromatid exchange formation in cultured Chinese hamster cells. Mutat Res. 1979 Aug;67(4):343–348. doi: 10.1016/0165-1218(79)90030-2. [DOI] [PubMed] [Google Scholar]
  17. McCann J., Choi E., Yamasaki E., Ames B. N. Detection of carcinogens as mutagens in the Salmonella/microsome test: assay of 300 chemicals. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5135–5139. doi: 10.1073/pnas.72.12.5135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Mondal S., Brankow D. W., Heidelberger C. Two-stage chemical oncogenesis in cultures of C3H/10T1/2 cells. Cancer Res. 1976 Jul;36(7 Pt 1):2254–2260. [PubMed] [Google Scholar]
  19. Mufson R. A., Laskin J. D., Fisher P. B., Weinstein I. B. Melittin shares certain cellular effects with phorbol ester tumour promoters. Nature. 1979 Jul 5;280(5717):72–74. doi: 10.1038/280072a0. [DOI] [PubMed] [Google Scholar]
  20. Nagasawa H., Little J. B. Effect of tumor promoters, protease inhibitors, and repair processes on x-ray-induced sister chromatid exchanges in mouse cells. Proc Natl Acad Sci U S A. 1979 Apr;76(4):1943–1947. doi: 10.1073/pnas.76.4.1943. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Olmsted J. B., Borisy G. G. Microtubules. Annu Rev Biochem. 1973;42:507–540. doi: 10.1146/annurev.bi.42.070173.002451. [DOI] [PubMed] [Google Scholar]
  22. Peraino C., Fry R. J., Staffeldt E. Reduction and enhancement by phenobarbital of hepatocarcinogenesis induced in the rat by 2-acetylaminofluorene. Cancer Res. 1971 Oct;31(10):1506–1512. [PubMed] [Google Scholar]
  23. Pollock E. J., Todaro G. J. Radiation enhancement of SV40 transformation in 3T3 and human cells. Nature. 1968 Aug 3;219(5153):520–521. doi: 10.1038/219520a0. [DOI] [PubMed] [Google Scholar]
  24. Rassoulzadegan M., Seif R., Cuzin F. Conditions leading to the establishment of the N (a gene dependent) and A (a gene independent) transformed states after polyoma virus infection of rat fibroblasts. J Virol. 1978 Nov;28(2):421–426. doi: 10.1128/jvi.28.2.421-426.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Reddy V. B., Thimmappaya B., Dhar R., Subramanian K. N., Zain B. S., Pan J., Ghosh P. K., Celma M. L., Weissman S. M. The genome of simian virus 40. Science. 1978 May 5;200(4341):494–502. doi: 10.1126/science.205947. [DOI] [PubMed] [Google Scholar]
  26. Rifkin D. B., Crowe R. M., Pollack R. Tumor promoters induce changes in the chick embryo fibroblast cytoskeleton. Cell. 1979 Oct;18(2):361–368. doi: 10.1016/0092-8674(79)90055-2. [DOI] [PubMed] [Google Scholar]
  27. Risser R., Pollack R. A nonselective analysis of SV40 transformation of mouse 3T3 cells. Virology. 1974 Jun;59(2):477–489. doi: 10.1016/0042-6822(74)90457-7. [DOI] [PubMed] [Google Scholar]
  28. Risser R., Pollack R. Factors affecting the frequency of transformation of rat embryo cells by simian virus 40. Virology. 1979 Jan 15;92(1):82–90. doi: 10.1016/0042-6822(79)90216-2. [DOI] [PubMed] [Google Scholar]
  29. Rose S. P., Stahn R., Passovoy D. S., Herschman H. Epidermal growth factor enhancement of skin tumor induction in mice. Experientia. 1976;32(7):913–915. doi: 10.1007/BF02003764. [DOI] [PubMed] [Google Scholar]
  30. STOKER M. EFFECT OF X-IRRADIATION ON SUSCEPTIBILITY OF CELLS TO TRANSFORMATION BY POLYOMA VIRUS. Nature. 1963 Nov 23;200:756–758. doi: 10.1038/200756a0. [DOI] [PubMed] [Google Scholar]
  31. Scher C. D., Nelson-Rees W. A. Direct isolation and characterization of "flat" SV40-transformed cells. Nat New Biol. 1971 Oct 27;233(43):263–265. doi: 10.1038/newbio233263a0. [DOI] [PubMed] [Google Scholar]
  32. Schlegel R., Benjamin T. L. Cellular alterations dependent upon the polyoma virus Hr-t function: separation of mitogenic from transforming capacities. Cell. 1978 Jul;14(3):587–599. doi: 10.1016/0092-8674(78)90244-1. [DOI] [PubMed] [Google Scholar]
  33. Segawa M., Nadamitsu S., Kondo K., Yoshizaki I. Chromosomal aberrations of Don lung cells of Chinese hamster after exposure to vinblastine in vitro. Mutat Res. 1979 Jan;66(1):99–102. doi: 10.1016/0165-1218(79)90012-0. [DOI] [PubMed] [Google Scholar]
  34. Seif R., Cuzin F. Temperature-sensitive growth regulation in one type of transformed rat cells induced by the tsa mutant of polyoma virus. J Virol. 1977 Dec;24(3):721–728. doi: 10.1128/jvi.24.3.721-728.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Seif R., Martin R. G. Growth state of the cell early after infection with simian virus 40 determines whether the maintenance of transformation will be A-gene dependent or independent. J Virol. 1979 Aug;31(2):350–359. doi: 10.1128/jvi.31.2.350-359.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. Seif R., Martin R. G. Simian virus 40 small t antigen is not required for the maintenance of transformation but may act as a promoter (cocarcinogen) during establishment of transformation in resting rat cells. J Virol. 1979 Dec;32(3):979–988. doi: 10.1128/jvi.32.3.979-988.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  37. Seif R. Polyoma virus middle t antigen: a tumor progression factor. J Virol. 1980 Aug;35(2):479–487. doi: 10.1128/jvi.35.2.479-487.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  38. Shin S. I., Freedman V. H., Risser R., Pollack R. Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. Proc Natl Acad Sci U S A. 1975 Nov;72(11):4435–4439. doi: 10.1073/pnas.72.11.4435. [DOI] [PMC free article] [PubMed] [Google Scholar]
  39. Smith H. S., Gelb L. D., Martin M. A. Detection and quantitation of simian virus 40 genetic material in abortively transformed BALB-3T3 clones (mice-diploid cells-virus equivalents). Proc Natl Acad Sci U S A. 1972 Jan;69(1):152–156. doi: 10.1073/pnas.69.1.152. [DOI] [PMC free article] [PubMed] [Google Scholar]
  40. Smith H. S., Scher C. D., Todaro G. J. Induction of cell division in medium lacking serum growth factor by SV40. Virology. 1971 May;44(2):359–370. doi: 10.1016/0042-6822(71)90267-4. [DOI] [PubMed] [Google Scholar]
  41. Soeda E., Arrand J. R., Griffin B. E. Polyoma virus. The early region and its T-antigens. Nucleic Acids Res. 1979 Oct 25;7(4):839–857. doi: 10.1093/nar/7.4.839. [DOI] [PMC free article] [PubMed] [Google Scholar]
  42. Stavrovskaya A. A., Kopnin B. P. Colcemid-induced polyploidy and aneuploidy in normal and tumour cells in vitro. Int J Cancer. 1975 Nov 15;16(5):730–737. doi: 10.1002/ijc.2910160505. [DOI] [PubMed] [Google Scholar]
  43. Stoker M. Abortive transformation by polyoma virus. Nature. 1968 Apr 20;218(5138):234–238. doi: 10.1038/218234a0. [DOI] [PubMed] [Google Scholar]
  44. TODARO G. J., GREEN H. ENHANCEMENT BY THYMIDINE ANALOGS OF SUSCEPTIBILITY OF CELLS TO TRANSFORMATION BY SV40. Virology. 1964 Nov;24:393–400. doi: 10.1016/0042-6822(64)90177-1. [DOI] [PubMed] [Google Scholar]
  45. Theile M., Scherneck S., Geissler E. Mutagenesis by simian virus 40. I. detection of mutations in Chinese hamster cell lines using different resistance markers. Mutat Res. 1976 Oct;37(1):111–123. doi: 10.1016/0027-5107(76)90059-2. [DOI] [PubMed] [Google Scholar]
  46. Theile M., Strauss M., Luebbe L., Scherneck S., Krause H., Geissler E. SV40-induced somatic mutations: possible relevance to viral transformation. Cold Spring Harb Symp Quant Biol. 1980;44(Pt 1):377–382. doi: 10.1101/sqb.1980.044.01.042. [DOI] [PubMed] [Google Scholar]
  47. Trosko J. E., Chang C. C., Yotti L. P., Chu E. H. Effect of phorbol myristate acetate on the recovery of spontaneous and ultraviolet light-induced 6-thioguanine and ouabain-resistant Chinese hamster cells. Cancer Res. 1977 Jan;37(1):188–193. [PubMed] [Google Scholar]
  48. VOGT M., DULBECCO R. Steps in the neoplastic transformation of hamster embryo cells by polyoma virus. Proc Natl Acad Sci U S A. 1963 Feb 15;49:171–179. doi: 10.1073/pnas.49.2.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  49. Vesselinovitch S. D., Mihailovich N. The inhibitory effect of griseofulvin on the "promotion" of skin carcinogenesis. Cancer Res. 1968 Dec;28(12):2463–2465. [PubMed] [Google Scholar]
  50. WOLMAN S. R., HIRSCHHORN K., TODARO G. J. EARLY CHROMOSOMAL CHANGES IN SV40-INFECTED HUMAN FIBROBLAST CULTURES. Cytogenetics. 1964;3:45–61. doi: 10.1159/000129797. [DOI] [PubMed] [Google Scholar]
  51. Weber K., Wehland J., Herzog W. Griseofulvin interacts with microtubules both in vivo and in vitro. J Mol Biol. 1976 Apr 25;102(4):817–829. doi: 10.1016/0022-2836(76)90293-x. [DOI] [PubMed] [Google Scholar]
  52. Wessells N. K., Spooner B. S., Ash J. F., Bradley M. O., Luduena M. A., Taylor E. L., Wrenn J. T., Yamada K. Microfilaments in cellular and developmental processes. Science. 1971 Jan 15;171(3967):135–143. doi: 10.1126/science.171.3967.135. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES