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. 1980 Nov;36(2):465–469. doi: 10.1128/jvi.36.2.465-469.1980

Virion nucleic acid of Ebola virus.

R L Regnery, K M Johnson, M P Kiley
PMCID: PMC353663  PMID: 7431486

Abstract

The virion nucleic acid of Ebola virus consists of a single-stranded RNA with a molecular weight of approximately 4.0 x 10(6). The virion RNA did not bind to oligodeoxythymidylic acid-cellulose under conditions known to bind RNAs rich in polyadenylic acid and was not infectious under conditions which yielded infectious RNA from Sindbis virus, suggesting that Ebola virus virion nucleic acid is a negative-stranded RNA.

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Selected References

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  1. Aviv H., Leder P. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose. Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408–1412. doi: 10.1073/pnas.69.6.1408. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bellamy A. R., Shapiro L., August J. T., Joklik W. K. Studies on reovirus RNA. I. Characterization of reovirus genome RNA. J Mol Biol. 1967 Oct 14;29(1):1–17. doi: 10.1016/0022-2836(67)90177-5. [DOI] [PubMed] [Google Scholar]
  3. Bishop D. H., Claybrook J. R., Spiegelman S. Electrophoretic separation of viral nucleic acids on polyacrylamide gels. J Mol Biol. 1967 Jun 28;26(3):373–387. doi: 10.1016/0022-2836(67)90310-5. [DOI] [PubMed] [Google Scholar]
  4. Bowen E. T., Lloyd G., Harris W. J., Platt G. S., Baskerville A., Vella E. E. Viral haemorrhagic fever in southern Sudan and northern Zaire. Preliminary studies on the aetiological agent. Lancet. 1977 Mar 12;1(8011):571–573. doi: 10.1016/s0140-6736(77)92001-3. [DOI] [PubMed] [Google Scholar]
  5. Bowen E. T., Simpson D. I., Bright W. F., Zlotnik I., Howard D. M. Vervet monkey disease: studies on some physical and chemical properties of the causative agent. Br J Exp Pathol. 1969 Aug;50(4):400–407. [PMC free article] [PubMed] [Google Scholar]
  6. Eaton B. T., Donaghue T. P., Faulkner P. Presence of poly (A) in the polyribosome-associated RNA of Sindbis-infected BHK cells. Nat New Biol. 1972 Jul 26;238(82):109–111. doi: 10.1038/newbio238109a0. [DOI] [PubMed] [Google Scholar]
  7. Johnson K. M., Lange J. V., Webb P. A., Murphy F. A. Isolation and partial characterisation of a new virus causing acute haemorrhagic fever in Zaire. Lancet. 1977 Mar 12;1(8011):569–571. doi: 10.1016/s0140-6736(77)92000-1. [DOI] [PubMed] [Google Scholar]
  8. Kissling R. E., Robinson R. Q., Murphy F. A., Whitfield S. G. Agent of disease contracted from green monkeys. Science. 1968 May 24;160(3830):888–890. doi: 10.1126/science.160.3830.888. [DOI] [PubMed] [Google Scholar]
  9. MANDEL B. THE EXTRACTION OF RIBONUCLEIC ACID FROM POLIOVIRUS BY TREATMENT WITH SODIUM DODECYL SULFATE. Virology. 1964 Mar;22:360–367. doi: 10.1016/0042-6822(64)90026-1. [DOI] [PubMed] [Google Scholar]
  10. McMaster G. K., Carmichael G. G. Analysis of single- and double-stranded nucleic acids on polyacrylamide and agarose gels by using glyoxal and acridine orange. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835–4838. doi: 10.1073/pnas.74.11.4835. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Newman J. F., Brown F. Absence of poly (A) from the infective RNA of Nodamura virus. J Gen Virol. 1976 Jan;30(1):137–140. doi: 10.1099/0022-1317-30-1-137. [DOI] [PubMed] [Google Scholar]
  12. Obijeski J. F., Bishop D. H., Murphy F. A., Palmer E. L. Structural proteins of La Crosse virus. J Virol. 1976 Sep;19(3):985–997. doi: 10.1128/jvi.19.3.985-997.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Pattyn S., van der Groen G., Jacob W., Piot P., Courteille G. Isolation of Marburg-like virus from a case of haemorrhagic fever in Zaire. Lancet. 1977 Mar 12;1(8011):573–574. doi: 10.1016/s0140-6736(77)92002-5. [DOI] [PubMed] [Google Scholar]
  14. Repik P., Bishop D. H. Determination of the molecular weight of animal RNA viral genomes by nuclease digestions. I. Vesicular stomatitis virus and its defective T particle. J Virol. 1973 Nov;12(5):969–983. doi: 10.1128/jvi.12.5.969-983.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Semancik J. S. Detection of polyadenylic acid sequences in plant pathogenic RNAs. Virology. 1974 Nov;62(1):288–291. doi: 10.1016/0042-6822(74)90325-0. [DOI] [PubMed] [Google Scholar]
  16. Weissmann C., Billeter M. A., Goodman H. M., Hindley J., Weber H. Structure and function of phage RNA. Annu Rev Biochem. 1973;42:303–328. doi: 10.1146/annurev.bi.42.070173.001511. [DOI] [PubMed] [Google Scholar]
  17. Wengler G., Wengler G., Gross H. J. Studies on virus-specific nucleic acids synthesized in vertebrate and mosquito cells infected with flaviviruses. Virology. 1978 Sep;89(2):423–437. doi: 10.1016/0042-6822(78)90185-x. [DOI] [PubMed] [Google Scholar]

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