Skip to main content
NCBI home page
Journal of Virology logoLink to Journal of Virology
. 1980 Nov;36(2):519–525. doi: 10.1128/jvi.36.2.519-525.1980

Characterization of tau antigens isolated from uninfected and simian virus 40-infected monkey cells and papovavirus-transformed cells.

D T Simmons
PMCID: PMC353669  PMID: 6253675

Abstract

Tau antigens (also known as cellular or nonviral tumor antigens) were detected in uninfected and simian virus 40-infected monkey cells after immunoprecipitation with serum from hamsters bearing simian virus 40-induced tumours (anti-T serum). These two proteins (56,000 daltons) were digested to similarly sized peptides with various amounts of Staphylococcus aureus V8 protease. The Tau antigen isolated from infected monkey cells was closely related but was not identical to the corresponding protein from human cells transformed by simian virus 40, as determined by two-dimensional mapping of their methionine-labeled tryptic peptides. Hamster cells transformed by various primate papovaviruses (simian virus 40, BK virus, and JC virus) synthesized indistinguishable Tau antigens, as determined by two-dimensional peptide mapping. When tested by the same procedure, these proteins and the ones made in monkey and human cells were found to be related to the Tau antigens isolated from simian virus 40-transformed mouse and rat cells. Based on these results, an "evolutionary tree" was constructed to show the relationship among the methionine-containing tryptic peptides of all of these proteins.

Full text

PDF
519

Images in this article

520Image
on p.520
521Image
on p.521
522Image
on p.522
523Image
on p.523
523Image
on p.523

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Chamberlain J. P. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate. Anal Biochem. 1979 Sep 15;98(1):132–135. doi: 10.1016/0003-2697(79)90716-4. [DOI] [PubMed] [Google Scholar]
  2. Chang C., Simmons D. T., Martin M. A., Mora P. T. Identification and partial characterization of new antigens from simian virus 40-transformed mouse cells. J Virol. 1979 Aug;31(2):463–471. doi: 10.1128/jvi.31.2.463-471.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cleveland D. W., Fischer S. G., Kirschner M. W., Laemmli U. K. Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gel electrophoresis. J Biol Chem. 1977 Feb 10;252(3):1102–1106. [PubMed] [Google Scholar]
  4. Crawford L. V., Pim D. C., Lane D. P. An immunochemical investigation of SV40 T-antigens. 2. Quantitation of antigens and antibody activities. Virology. 1980 Jan 30;100(2):314–325. doi: 10.1016/0042-6822(80)90522-x. [DOI] [PubMed] [Google Scholar]
  5. DeLeo A. B., Jay G., Appella E., Dubois G. C., Law L. W., Old L. J. Detection of a transformation-related antigen in chemically induced sarcomas and other transformed cells of the mouse. Proc Natl Acad Sci U S A. 1979 May;76(5):2420–2424. doi: 10.1073/pnas.76.5.2420. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Edwards C. A., Khoury G., Martin R. G. Phosphorylation of T-antigen and control T-antigen expression in cells transformed by wild-type and tsA mutants of simian virus 40. J Virol. 1979 Feb;29(2):753–762. doi: 10.1128/jvi.29.2.753-762.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gaudray P., Rassoulzadegan M., Cuzin F. Expression of simian virus 40 early genes in transformed rat cells is correlated with maintenance of the transformed phenotype. Proc Natl Acad Sci U S A. 1978 Oct;75(10):4987–4991. doi: 10.1073/pnas.75.10.4987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Kress M., May E., Cassingena R., May P. Simian virus 40-transformed cells express new species of proteins precipitable by anti-simian virus 40 tumor serum. J Virol. 1979 Aug;31(2):472–483. doi: 10.1128/jvi.31.2.472-483.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lane D. P., Crawford L. V. T antigen is bound to a host protein in SV40-transformed cells. Nature. 1979 Mar 15;278(5701):261–263. doi: 10.1038/278261a0. [DOI] [PubMed] [Google Scholar]
  10. Linzer D. I., Levine A. J. Characterization of a 54K dalton cellular SV40 tumor antigen present in SV40-transformed cells and uninfected embryonal carcinoma cells. Cell. 1979 May;17(1):43–52. doi: 10.1016/0092-8674(79)90293-9. [DOI] [PubMed] [Google Scholar]
  11. Linzer D. I., Maltzman W., Levine A. J. The SV40 A gene product is required for the production of a 54,000 MW cellular tumor antigen. Virology. 1979 Oct 30;98(2):308–318. doi: 10.1016/0042-6822(79)90554-3. [DOI] [PubMed] [Google Scholar]
  12. McCormick F., Harlow E. Association of a murine 53,000-dalton phosphoprotein with simian virus 40 large-T antigen in transformed cells. J Virol. 1980 Apr;34(1):213–224. doi: 10.1128/jvi.34.1.213-224.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Melero J. A., Stitt D. T., Mangel W. F., Carroll R. B. Identification of new polypeptide species (48-55K) immunoprecipitable by antiserum to purified large T antigen and present in SV40-infected and -transformed cells. Virology. 1979 Mar;93(2):466–480. doi: 10.1016/0042-6822(79)90250-2. [DOI] [PubMed] [Google Scholar]
  14. Melero J. A., Tur S., Carroll R. B. Host nuclear proteins expressed in simian virus 40-transformed and -infected cells. Proc Natl Acad Sci U S A. 1980 Jan;77(1):97–101. doi: 10.1073/pnas.77.1.97. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Simmons D. T., Martin M. A. Common methionine-tryptic peptides near the amino-terminal end of primate papovavirus tumor antigens. Proc Natl Acad Sci U S A. 1978 Mar;75(3):1131–1135. doi: 10.1073/pnas.75.3.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Simmons D. T., Martin M. A., Mora P. T., Chang C. Relationship among Tau antigens isolated from various lines of simian virus 40-transformed cells. J Virol. 1980 Jun;34(3):650–657. doi: 10.1128/jvi.34.3.650-657.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Smith A. E., Smith R., Paucha E. Characterization of different tumor antigens present in cells transformed by simian virus 40. Cell. 1979 Oct;18(2):335–346. doi: 10.1016/0092-8674(79)90053-9. [DOI] [PubMed] [Google Scholar]
  18. Takemoto K. K., Martin M. A. Transformation of hamster kidney cells by BK papovavirus DNA. J Virol. 1975 Jan;17(1):247–253. doi: 10.1128/jvi.17.1.247-253.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Takemoto K. K., Mullarkey M. F. Human papovavirus, BK strain: biological studies including antigenic relationship to simian virus 40. J Virol. 1973 Sep;12(3):625–631. doi: 10.1128/jvi.12.3.625-631.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Todaro G. J., Green H., Swift M. R. Susceptibility of human diploid fibroblast strains to transformation by SV40 virus. Science. 1966 Sep 9;153(3741):1252–1254. doi: 10.1126/science.153.3741.1252. [DOI] [PubMed] [Google Scholar]
  21. Walker D. L., Padgett B. L., ZuRhein G. M., Albert A. E., Marsh R. F. Human papovavirus (JC): induction of brain tumors in hamsters. Science. 1973 Aug 17;181(4100):674–676. doi: 10.1126/science.181.4100.674. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Virology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES